Phytochemical Analysis, Multi-Element Composition and Biological Activities of Extracts and Lupenone from Albizia inundata (Mart.) Barneby & J.W.Grimes

In this study, antimicrobial activity of extracts and lupenone from A. inundata (Fabaceae, Mimosoideae), were tested. In addition, the multi-element composition of the leaves, bark and stems (heartwood) of A. inundata was evaluated using inductively coupled plasma optical emission spectrometry (ICP OES). Lupenone was isolated in the petroleum ether extract of leaves, for the first time. Biological assays were conducted to evaluate the antimicrobial (antibacterial, antifungal and antiparasitic) of extracts, their fractions and lupenone. The results showed antimicrobial and antileishmanial activity of these compounds, which may be useful for obtaining a chemical composition and biological activity database on Albizia species. For multi-element composition, we found the mean concentrations (minimum-maximum) of Al, Ca, Cr, Cu, Fe, K, Mg, Mn, Ni, P, V and Zn in the samples ranged between 0.06 and 265.41 μg g. Concentration ranges of Cu, Fe, Mn and Zn are large, which makes these samples as additional sources of these macronutrients.


Introduction
The genus Albizia belongs to the Fabaceae family, subfamily Mimosoideae, includes about 140 species, mainly woody trees and shrubs native to tropical Asia, also found in South Africa and South America. [1][2][3][4] The genus Albizia includes several species including the native congeners A. hassleri, A. niopoides, A. inundata, A. pedicellaris, A. polycephala and A. polyphylla, distributed throughout Brazil, recommended for the reforestation of areas degraded by have characteristics of rapid botanical growth. Some species of this genus have been reported to produce a wide aerial parts of Albizia inundata, and their bioactivity against human colon cells (HCT116) and melanoma cells (B16F10 and SKMEL28). 14,15 In literature, there are no reports of antibacterial, antifungal, antitrypanocidal and antileishmanial activities for A. inundata.
Chemical aspects and biological activity of A. inundata have been poorly explored, despite the potential of this plant as a source of bioactive compounds. In vitro cellular cytotoxicity assays have as main parameter to provide information on the safety of the various drugs or compounds, being the first step to evaluate the biocompatibility of any substance for the biomedical use. 16 Determination of inorganic components in medicinal plants is of great interest because chemical elements can be catalysts of biochemical reactions in plants and to influence in their therapeutic action on the human health. 17,18 Most multi-element analysis, in biological samples, require pretreatment of the samples prior to quantification by analytical techniques, such as, inductively coupled plasma optical emission spectrometry (ICP OES); inductively coupled plasma mass spectrometry (ICP-MS); atomic absorption spectrometry with graphite furnace (GF AAS), flame (FAAS) and hydride generation (HG AAS). [19][20][21][22][23] ICP OES is a sensitive multi-element technique, used in the analysis of biological samples due to its advantages, which consists of the quantification of elements through the emission of electromagnetic radiation, generated from the excitation of their atoms or ions. 24 In the literature, there are no reports of macro and micronutrient evaluation in species of the genus Albizia, which may contribute to the knowledge of their inorganic constituents.
In this study, lupenone was isolated in the petroleum ether extract of leaves from Albizia inundata (Mart.) Barneby & J.W.Grimes, for the first time, by chromatographic techniques and identified by one-dimensional nuclear magnetic resonance ( 1 H and 13 C NMR). For evaluation of antimicrobial activities (antibacterial, antifungal and antiparasitic) of extracts and lupenone from A. inundata, biological assays and ultrastructural analysis were conducted. In addition, ICP OES was used for multi-element analysis in aerial parts of A. inundata. This work is relevant to expand the studies about chemical and biological potential of this species and to evaluate the multi-element composition of the leaves, bark and stems (heartwood) of A. inundata.

Plant materials and preparation of plant extracts
The aerial parts of Albizia inundata (Mart.) Barneby  Dried aerial parts, leaves (574.80 g); stems (405.28 g) and stem bark (1004.70 g) of A. inundata were ground and cut (stem/core) into small pieces, with a knife mill (Marconi-MA680; Macro Vertical Rotor Mill with fixed and mobile knives, Piracicaba, Brazil) and subjected to the successive extraction by the maceration process with petroleum ether, hexane and methanol (Vetec and Synth, analytical grade, São Paulo, Brazil), with three successive exchanges for each solvent, every 72 h. After, the solvents were removed using a rotary evaporator under reduced pressure (IKA ® RV 10 digital, IKA® HB 10 Basic, Rio de Janeiro, Brazil), at a temperature of 30-55 °C, to obtain the respective crude methanolic extracts: leaves (EML), stem bark (SMSB), stem (SEM), and petroleum ether of the leaves (EPEL), with 12.0, 3.30, 2.09 and 3.14% yield, respectively.

General experimental procedures
Initially, the extracts were subjected to silica gel 60 chromatography (70-230 mesh, Merck and Vetec, São Paulo, Brazil) and comparative thin layer chromatography (TLC) techniques using aluminum chromate plates coated with silica gel, 0.20 mm, with fluorescent indicator UV254/365 (Macherey-Nagel ® ; Merck, São Paulo, Brazil), as well as preparative thin layer chromatography (CCDP) on glass plates of size 20 × 20 cm, prepared with silica gel 60 (PF254-366, Merck, São Paulo, Brazil). All the chemical reagents used were purchased from Merck (Darmstadt, Germany) and Sigma Chemical Co. (St. Louis, MO, USA) and were of analytical grade. The water was purified using the Milli-Q purification system from Millipore (Bedford, MA, USA).
The petroleum ether extract from the leaves (initial mass 18.03 g) of A. inundata was selected for continuation of the fractionation studies. The NMR spectra were obtained in Agilent DD2 (Santa Clara, CA, USA) spectrometers operating at 500 MHz ( 1 H) and, 75 and 125 MHz ( 13 C). The samples were solubilized in deuterated chloroform (CDCl 3 ), having the internal reference standard (d = 0 ppm), tetramethylsilane (TMS) for 1 H NMR and CDCl 3 for 13 C NMR. Chemical shifts were expressed in ppm values (d) given in relation to TMS. The coupling constants (J) were measured in hertz (Hz).

Antibiotic and antifungal tests
Broth microdilution method was used for determination of the minimum inhibitory concentration (MIC) according to the technical standards of the Clinical and Laboratory Standards Institute, with modifications. 25,26 This broth microdilution assay was performed under aseptic conditions using a sterile 96 well plate. Stock solutions of the extracts in petroleum ether, fraction EPEL10, and methanol from the leaves, stems and stem bark were prepared in the dimethyl sulfoxide (DMSO) in the concentration of 2000 μg mL -1 (lupenone of 400 μg mL -1 ). The final concentrations of the extracts and fraction EPEL10 were tested in the range of 500 to 3.9 μg mL -1 and the lupenone in the 100 to 0.78 μg mL -1 . The positive controls used were, respectively: benzylpenicillin (1 mg mL -1 ) for Gram-positive bacteria, geramycin (200 μg mL -1 ) for Gramnegative bacteria and ciclopirox olamine (400 μg mL -1 ) for fungus. The inoculum preparation was performed based on turbidity (0.5 on the McFarland scale, approximately 1.5 × 10 8 colony-forming unit (CFU) mL -1 ). The fungi were incubated for 72 h at 26 °C and bacteria in 24 h at 36 °C. The MIC was determined as the lowest concentration capable of visually inhibiting the growth of the microorganisms. The experiment was performed in triplicate. The assay to obtain the minimal microbicidal concentrations (MBC, for bacteria and MFC, for fungus) were conducted according to Souza-Neta and co-workers, 26 without modifications. Subsequently, 20 μL per well of AlamarBlue (Invitrogen, Carlsbad, CA, USA) was added for 2 h. The readings were performed using a spectrophotometer at 570 and 600 nm wavelengths. The percent inhibition of the axenic culture was determined based on the untreated control. The inhibitory concentration for 50% (IC 50 ) was calculated based on the percent inhibition of parasite growth, related to negative controls, and accessed through concentration logarithm values followed by nonlinear regression curve fit.

In vitro macrophage infection and treatment
To evaluate the effect of the lupenone in the intracellular amastigote form, J774 macrophages (mouse macrophage-like cell line) were cultured in 24-well plates (2 × 10 5 per well) and infected with stationary-phase promastigotes of L. amazonensis at a ratio of 10:1, for 4 h. Infected macrophages were treated with different concentrations of lupenone for 24 h, in triplicate. Amphotericin B was used as a positive control. The cells were fixed in methanol and stained by Giemsa (Sigma-Aldrich, St. Louis, MO, USA). The percentage of infected macrophages and the number of amastigotes by macrophages were determined by counting 100 cells per slides, as previously described. 27 Ultrastructural analysis J774 macrophages infected with L. amazonensis were treated with lupenone for 24 h at 37 °C and 5% CO 2 . After incubation, the parasites were fixed for 1 h at room temperature with 2% formaldehyde and 2.5% glutaraldehyde (Electron Microscopy Sciences, Hatfield, PA, USA) in sodium cacodylate buffer (0.1 M, pH 7.2). After fixation, the parasites were washed 4 times with 0.1 mM sodium cacodylate buffer, pH 7.2, and post-fixed with a 1% solution of osmium tetroxide (Sigma Chemical Company, St. Louis, MO, USA). Cells were subsequently dehydrated in increasing concentrations of acetone (30,50,70, 90 and 100%) for 10 min at each step and embedded in Polybed resin (PolyScience family, Warrington, PA, USA). Ultrafine sections prepared using a Leica UC7 ultramicrotome, were collected, contrasted with uranyl acetate and lead citrate and observed under a JEOL TEM-1320 (JEOL, Tokyo, Japan) transmission electron microscope.

Trypanocidal activity
Trypomastigotes collected from the supernatants of LLC-MK2 cells were dispensed into 96-well plates at a cell density of 4 × 10 5 cells per well. Screening was performed with all the compounds in a single concentration of 25 μg mL -1 . The plate was incubated for 24 h at 37 ºC and 5% of CO 2 . Aliquots of each well were collected and the number of viable parasites, based on parasite motility, was assessed in a Neubauer chamber. The percentage of inhibition was calculated in relation to untreated cultures. This experiment was repeated once, and benznidazole was used as the positive control.

Cytotoxicity evaluation
To determine the cytotoxicity of the extracts and lupenone, J774 cells were seeded into 96-well plates at a cell density of 1 × 10 4 cells per well in Dulbecco's modified Eagle medium (DMEM; Life Technologies, GIBCO-BRL, Gaithersburg, MD, USA) supplemented with 10% FBS (Gibco Invitogen Co., Tastrup, Denmark), and 50 μg mL -1 of gentamycin (Novafarma, Anápolis, GO, Brazil) and incubated for 24 h at 37° C and 5% CO 2 . After that time, each test inhibitors were added at least in five concentrations, in triplicate, and incubated for 72 h. Twenty μL per well of AlamarBlue (Invitrogen, Carlsbad, CA, USA) were added to the plates during 4 h. Colorimetric readings were performed at 570 and 600 nm using a spectrophotometer. CC 50 values (cytotoxicity concentration at 50%) were calculated using data-points gathered from two independent experiments.

Preparation and multi-element analysis
The acid digestion of A. inundata samples-leaves, bark and stems (heartwood)-was performed using a commercial high-pressure laboratory microwave oven (Milestone Ethos 1600 Microwave Labstation, Sorisole, Italy) operating at a frequency of 2450 Hz, with an energy output of 900 W. This microwave digestion system was equipped with ten 100-mL tetrafluoromethoxy vessels and a ceramic vessel jacket. The maximum operating temperature and pressure were 180 °C, 1000 W and 35 bar, respectively, in 30 min. The method used to determine the mineral composition of A. inundata samples was quick and simple, according to Santos Jr. et al.: 28 approximately 0.25 g of each sample was inserted directly into a microwave-closed vessel and 7 mL of 65% (m m -1 ) HNO 3 were added for 12 h in a predigestion at room temperature (25 °C). Then, 0.5 mL of 30% (m m -1 ) H 2 O 2 were added to each vessel and subjected to digestion. After cooling, the samples were then diluted to 10 mL. Metal contents of the final solution were determined by ICP OES.
An inductively coupled plasma optical emission spectrometer (ICP OES, VISTA PRO, Varian-Mulgrave, Australia) with axial viewing and a solid state detector was used for the simultaneous determination of the analytes of interest (Al, As, Ba, Ca, Cd, Co, Cr, Cu, Fe, Hg, K, Mg, Mn, Mo, Ni, P, Pb, Se, V and Zn). The optical system was calibrated using a multi-element stock solution for ICP OES, whereas a 5.0 mg L -1 Mn standard solution was used for optical alignment. The spectral lines were selected according to the absence of spectral interference and appropriate sensitivity for determining elements at high and low concentrations, by studying the emission lines of the elements to be investigated. The lines that exhibited low interference, high analytical signal and background ratios were selected. The optimized parameters of ICP OES were: radio frequency power (RF) generator power (1300 W); plasma gas rate (1.5 mL min -1 ); auxiliary gas rate (1.5 mL min -1 ); nebulizer gas rate (0.7 mL min -1 ); sample uptake rate (0.8 mL min -1 ); injector tube diameter

Validation studies
The method was validated by performance parameters: stability, linearity, precision, accuracy, limit of detection, limit of quantification and matrix effect. 29 Samples and multi-element solution stability were evaluated for 24 h at room temperature and kept at 37 ºC for 2 h in 0.01 and 0.1 mol L -1 HCl by checking changes in the analytical signal after analyzed by ICP OES. Linearity was evaluated by linear regression of analytical curves (1.0 to 200.0 μg mL -1 for macro-and 1.0 to 50.0 μg mL -1 for microelements). Precision was evaluated through relative standard deviation (RSD) from the obtained data by analyzing solutions of macro-and microelements with a working concentration of 5 and 25 μg mL -1 , in six replicates.
The accuracy of the measurements was assessed using apple leaves certified reference material 1515 (CRM/NIST 1515) from the National Institute of Standards and Technology (Gaithersburg, MD, USA). In addition, a recovery study was conducted by adding known amounts (5 μg) of each mineral in samples, for evaluation of accuracy. Limits of detection (LOD) and quantification (LOQ) were calculated using the concentration equivalent to three times the standard deviation (3σ) of the signal (n = 10) of the analytical blank solution, and the LOQ was calculated using 10σ criterion (n = 10), respectively. The matrix effect was evaluated by comparing the slopes of curves obtained by external calibration with standard solutions in acid medium (2 mol L -1 HNO 3 ), from solutions of the digested samples by ICP OES.

Statistical analysis
The IC 50 of leishmania growth and CC 50 were calculated based in a nonlinear regression (curve fit) and the statistical analyses were made by one-way analysis of variance (ANOVA) and Newman-Keuls multiple comparison test using Prism 5.02 GraphPad Software. 30 The critical level of significance was established for p < 0.05. All experiments were done at least twice.

Results and Discussion
Identification structural of the lupenone The structural identification of lupenone in the subfractions F3.24.4, F3.28.3 and F3.29.3 was performed analyzing their 1 H and 13 C NMR data (Supplementary Information (SI) section) and comparing it with those available in the literature. [31][32][33][34][35] The 1 H NMR spectrum of the compound ( Figure S1a, SI section) showed characteristic triterpenoid signals between d H 0.67-1.92, in addition to two broad singlets at d H 4.58 and 4.70, typical for double bond reference to geminal olefinic hydrogens in C-29 and it can be concluded that it is a lupane skeleton. 31 Further, the spectrum indicated the presence of seven methyl singlets at d H 0.76, 0.81, 0.89, 0.94, 0.95, 1.03 and 1.69 ( Figure S1b, SI section). These signals are in accordance with those of the literature for lupenone. 32 The 13 C NMR spectrum displayed the signal at d C 218.2 ( Figure S2a, SI section) characteristic of carbonyl, and the signals in d C 109.4 and 150.9 for olefinic carbons. 33 It was also possible to identify chemical shifts of the following primary carbons: d C 26.6; 21.0; 15.8; 16.0; 14.5; 18.0 and 19.7, representing the carbons C23, C24, C25, C26, C27, C28 and C30, respectively ( Figure S2b, SI section). After analyzing these spectral data and comparing them with the literature (Table 1) it was possible to identify that fractions F3.24.4, F3.28.3 and F3.29.3 are constituted by lupenone (Figure 1). 34,35 Although lupenone has already been found in other species of Albizia (A. versicolor, A. schimperana and Albizia gummifera) 36 and A. lebbeck, 37 its isolation in A. inundata is being reported for the first time, in this study.
The 1 H NMR spectra of the methanol extracts of the stem bark and leaves ( Figures S3 and S4, SI section) showed signals for protons of the structural skeleton of derivatives triterpenes and steroids, among other metabolites, at d H 3.06-3.51 (carbinolic protons); at d H 4.98-5.56 (olefinic protons); d H 1.98-0.76 and d H 1.07-3.5 (methyl and methylenic protons, respectively). Other signals at d H 4.40-5.50 suggest presence of the anomeric protons. In addition to these, aromatic protons at d H 6.87-8.0, mainly the 1 H NMR spectrum of the stem bark extract, which was most active, indicate the presence of the classes of the aromatic secondary metabolites ( Figure S4, SI section).
A. inundata is still an unexplored species. In the literature, only Zhang et al. 14 identified pentacyclic saponins isolated from the methanolic extract of A. inundata bark. Chemical investigations related to other Albizia species have led to the isolation of lignans, flavonoids, alkaloids, saponins, diterpenes, and others. Some species have been reported producing a wide range of triterpenoid saponins by Liu et al. 4 Cao et al. 6 isolated three new oleanane triterpenoid saponins from the root of A. gummifera. Ikeda et al. 8 isolated and elucidated the structures of three new saponins, julibrosidas from A. julibrissin. In the first report of saponin esters in dicotyledonous plants, Krief et al. 7 isolated three new oleanane triterpenoid saponins, called A-C grandibracteosides, from the methanol extract from leaves of A. grandibracteata, a species widely consumed by primates in the Kibale/Uganda National Park.

Cytotoxicity assessment
In the Albizia genus, plants are known for their traditional medicinal uses for the treatment of mental disorders, insomnia, tumors, among other diseases. 38,39 The plant extract and lupenone were evaluated against mammalian cells to determine the cytotoxicity. The petroleum ether extracts did not demonstrate cytotoxicity (CC 50 values > 100 μg mL -1 ). Lupenone exhibited CC 50 = 31.95 μg mL -1 , being several times less cytotoxic in comparison with gentian violet (CC 50 = 1.16 ± 0.7 μg mL -1 ), the reference drug in this assay. In contrast, for the methanolic extracts of the bark, stem and leaves were obtained CC 50 values of 10.26 (± 0.6), 12.52 (± 0.7) and 1.567 (± 0.9) μg mL -1 , respectively. These results show potential cytotoxic of these compounds. In a previous report 14 was demonstrated that two triterpenic saponins isolated from A. inundata presented activity against tumor cells, with IC 50 of 1.0-3.8 μg mL -1 . These data suggest a possible antitumor activity of the methanolic extracts and lupenone isolated from A. inundata.
In this study, antitumor activity of extracts and lupenone was not evaluated. In literature, Zhang et al. 14 carried out studies on the pharmacological potential of A. inundata against tumor melanoma cells (B16F10 and SKMEL28), from two triterpenic saponins isolated from the methanolic extract from the aerial parts, obtaining the IC 50 value of 1.0-3.8 μg mL -1 , with satisfactory results regarding the other substances analyzed in the research against the same tumor cell lines. In addition, Wei et al. 15 evaluated the cytotoxic activity of oleanane triterpenoid saponins against human colon cells (HCT116). Therefore, there are perspectives  Melek et al. 10 performed a phytochemical and biological study of the A. procera species and highlighted cytotoxicity against HEPG2 cells with IC 50 values of 9.3 and 10 μg mL -1 . Lacroix et al. 11 reported cytotoxicity against human tumor cells from squamous cell carcinoma of the mouth (KB), obtaining IC 50 value of 21 μg mL -1 , from extracts from the leaves of A. grandibracteata.

Antibacterial and antifungal activities
The results for antibacterial and antifungal activities of A. inundata (petroleum ether extract from leaves; extracts in methanol from the leaves, stems and stem bark and of lupenone, isolated of the petroleum ether extract from leaves) was evaluated against three Gram positive, three Gram negative strains and Candida albicans. Minimum bactericidal concentration (CBM) and minimum fungicidal concentration (CFM) are presented in Table 2.
Among the samples tested, the methanolic extract of the stem bark (SMSB) was the most active against all the microorganisms tested, with MICs values in the range of 3.9-125 μg mL -1 . Although this extract had low bacteriostatic selectivity, its bactericidal effect on Gram-negative bacteria S. choleraesuis and P. aeruginosa was elevated with values of MMCs of 62.5 and 125 μg mL -1 , respectively. These antibacterial effect of the extracts can probably be inferred by the presence of triterpenic saponins, among other metabolites, according to a preliminary phytochemical study of aerial parts of a specimen of A. inundata. 15 The antimicrobial assay demonstrated that the methanol extract from the leaves (EML) showed activity against S. choleraesuis, B. subtilis, M. luteus and C. albicans with MICs of 250, 125, 15.63 and 250 μg mL -1 , respectively.
The petroleum ether extract from the leaves showed no antibacterial effect, however, lupenone, which was isolated from this extract, showed selective bacteriostatic effect for M. luteus with MIC of 50 μg mL -1 . The synergism of the compound mixture masked the antimicrobial effect of this extract. Lupenone has been known to have anti-inflammatory, anti-diabetic, antitumor and, antiviral activity, 40 but the bacteriostatic effect on M. luteus has not been reported.
lupenone was several times less cytotoxic in comparison with amphotericin B (Table 3). The extracts did not show antileishmanial activity. In literature, there are no reports on antileishmanial activities of A. inundata and there are few data on the effects of terpenes against different species of leishmania. [41][42][43] Monoterpenes isolated from essential oils and aromatic plants presented IC 50 for promastigotes higher than those found in the present work (48-65 μM). 44 α-Amyrin, 11-oxo-α-amyrone and 11-oxo-α-amyrin, triterpenes obtained from Cola nitida, presented an IC 50 values against promastigotes of L. amazonensis of 19.42 and 11.6 μg mL -1 , respectively. The lipophilicity of triterpenes might facilitate penetration through lipid bilayer of cellular membranes, increasing the toxicity against leishmania species. 45 To evaluate the effects of lupenone against amastigotes, J774 macrophages were infected with L. amazonensis promastigotes and treated at different concentrations (5, 10 and 20 μM) of the compound for 24 h. Although there was no significant reduction in the percentage of infected macrophages at all concentrations tested, treatment with lupenone significantly reduced the number of amastigotes per macrophage at a concentration of 20 μM (Figure 2). Lupenone presented an IC 50 value of 20.6 μM against amastigotes. The lupenone was more effective than lupeol, isolated from aerial parts of Vernonia scorpioides, which showed an IC 50 of 40.73 μg mL -1 against amastigotes of L. amazonensis. 46 In another study, α-bisabolol terpene showed an IC 50 of 10.70 μg mL -1 against amastigotes of L. amazonensis, with higher IC 50 values than lupenone. 47 Lupenone and extracts were tested in initial screening against Trypanosoma cruzi trypomastigotes, and no activity was observed (data not shown).
To evaluate the effects of lupenone on the parasite structures, J774 macrophages were infected with L. amazonensis and treated with lupenone at 20 μM. After 24 h of treatment, ultrastructural changes were evaluated by the transmission electron microscopy. Untreated parasites showed normal morphology, without alterations (Figures 3a  and 3b). The presence of lipid inclusions was observed in the cytoplasm of amastigotes treated with lupenone, suggesting an accumulation of abnormal lipids and precursors in the parasite (Figures 3c and 3d). 47 Interestingly, similar alterations were found in promastigotes of L. amazonensis treated with an essential oil from Vanillosmopsis arborea and (-)α-bisabolol, an alcohol sesquiterpene. 48,49 The appearance of lipids inclusions indicates that lupenone might affect the lipid biosynthesis pathways, a promise target in the leishmania drug discovery field. 50,51   were determined after 24 h of treatment. Amphotericin B (Amph B; 5 μM) was used as a reference drug. *P < 0.05 compared to C+ group; ** P < 0.01 compared to C+ group; *** P < 0.001 compared to C+ group, ns: not significant.

Multi-element analysis
The method was validated for stability and, samples and multi-element solutions were stable within 24 h. Linearity was evaluated and a good linearity was obtained for all observed lines, with determination coefficients (R 2 ) in the range from 0.9994 to 0.9998. Precision was assessed and the relative standard deviation (RSD) values were lower than 10%, indicating the high precision of this method.
A recovery test of the analytical procedure was carried out for the elements under study in leaves of A. inundata by spiking (5 μg of each analyte) the analyzed samples with aliquots of metal standards and then reanalyzing the samples. This sample was chosen for this test due to its available mass. The obtained recovery values, in the range of 90-107%, showed that there are no signs of systematic errors due to operation effects of the added analyte, such as losses during the process. The results showed good agreement with the reference values of the CRM/NIST 1515-apple leaves ( Table 4). The obtained recovery values, in the range of 90 to 110%, showed good accuracy of the method. It was possible to verify similarities in the amount of macro and microelements when comparing the values obtained in the CRM 1515 with the leaves sample of A. inundata, mainly for the elements Al, K, Mg and V. It is suggested that this similarity can be attributed to the same part of the plant, of both materials. When analyzing the bark and the stem it was possible to establish similarities for the micronutrients Cr, K, P and Zn, present in the studied plant.
The LODs and LOQs obtained for Al, As, Ba, Ca, Cd, Co, Cr, Cu, Fe, Hg, K, Mg, Mn, Mo, Ni, P, Pb, Se, V and Zn determined by ICP OES are shown in Table 4. The residual acidity of the digests was < 3.0 mol L -1 . The slopes of the calibration curves for each element do not show significant variations, at 95% confidence. These results showed that the matrix effect is not significant for the measures in ICP OES under the selected operating conditions.
The content (μg g -1 or mg g -1 ) of 19 elements (Al, As, Ba, Ca, Cd, Co, Cr, Cu, Fe, Hg, K, Mg, Mn, Mo, Ni, P , Pb, V and Zn) in the areas (leaf, bark and stem/heartwood) of the Albizia inundata plant was determined. As, Ba, Cd, Co, Hg, Mo e Pb, in all the investigated samples, were found to be below the LOD value of ICP OES.
No data were found in the literature for Albizia species, regarding elemental composition. The results indicated that the aerial parts of A. inundata contain amounts of Al, Ca, Cr, Cu, Fe, K, Mg, Mn, Ni, P, V and Zn ( Table 4). Concentrations of Al, Cr, Cu, Fe and Mn varied widely in the samples studied. The results obtained for the macro (K and P) and micronutrients (Al, Cr, Cu, Fe and Zn), present in the aerial parts of A. inundata, showed that leaves presented the highest values of these elements, when compared to the other parts of the plant. These variations of concentration may have occurred due to variations in the distribution of these elements in the biological cycle in the plant, their incorporation into the aerial structures of A. inundata, changes in soil and other geoclimatic conditions, among other variables. 52 Especially for Cr, it is suggested that some type of soil or sample contamination may have occurred during collection, transport, packaging and/or preparation. Elci et al. 53 proposed a selective extraction of chromium(VI) using a leaching procedure with sodium carbonate from some plant leaves, soil and sediment samples by graphite furnace atomic absorption spectrometry (GF AAS), after acid digestion with mixture of HClO 4 + HNO 3 + H 2 O 2 of plants (tomato and fig leaves). The authors quantified a high Cr VI and total chromium content in growing tomato and fig leaves, in soil of land close to the leather tanning industry region. Altundag et al., 54 in Sakarya, Turkey, studied the influence of selected soil and plant properties and their potential impacts on human health in urban environments by two artificial digestion models (stomach and intestinal). Soil and plants samples were dissolved by an acid mixture of HNO 3 (65%)/HCl (37%). The authors concluded that trace element levels transmitted from soil and plants (parsley and lettuce) to humans were low, in range of 0 and 68% for Al and Cd, respectively.
Establishing the multi-element composition of plants with therapeutic potential is necessary to contribute to the health organs because the plants may be used as sources of nutrients to animal and human health. In literature, studies have determined multi-element in plants and others biological samples by spectroscopic techniques. Marques and Nóbrega 55 developed a fast and simple flow-batch extraction procedure for screening of macro and micronutrients in dried plant leaves by ICP OES. Barin et al. 56 Table 4. Concentrations of Ca, K, Mg, P, Al, Cr, Cu, Fe, Mn, Ni, V and Zn in leaves, bark and stem (heartwood) from A. inundata and CRM/NIST 1515-apple leaves, after digestion in microwave oven. Values obtained for LOD and LOQ, by digestion in a microwave oven (mean ± standard deviation, n = 3, 95% confidence level) 286.0 ± 9.0 1.5 ± 0.