Anti-Parasite Activity of Novel 3 , 5-Diiodophenethyl-benzamides

Novel iodotyramides with para-substituted benzoic acids were synthesized via electrophilic aromatic substitutions and amide coupling via N,N’-diisopropylcarbodiimide (DIC) in dimethylformamide (DMF). All derivatives were in vitro screened against U-937 macrophages and Plasmodium falciparum, Leishmania panamensis and Trypanosoma cruzi protozoan parasites. The hemolytic activity was evaluated on human red blood cells (RBC). Compounds N-(4-hydroxy-3,5-diiodophenethyl)-4-methylbenzamide and N-(3,5-diiodo4-methoxyphenethyl)-4-methoxybenzamide were the most active against L. panamensis with an effective concentration 50 (EC50) of 17.9 and 17.5 μg mL, respectively; while compounds N-(3,5-diiodo-4-methoxyphenethyl)-4-methylbenzamide and N-(3,5-diiodo-4-methoxyphenethyl)4-methoxybenzamide were the most active for T. cruzi with EC50 values of 23.75 and 6.19 μg mL, respectively. In contrast, all derivatives showed high activity against P. falciparum with EC50 < 25 μg mL, except compound N-(4-hydroxy-3,5-diiodophenethyl)-benzamide. No compound was hemolytic over RBC. This report represents the importance of novel iodotyramides as anti-parasites agents.


Introduction
Marine organisms represent a large source of primary and secondary bioactive metabolites.Halogenated alkaloids, especially those with iodine atoms, are a type of secondary metabolites with diverse biological activities.While iodinated halometabolites are hard to find in nature, chloro and bromo derivatives alkaloids are more common. 1Nevertheless, ten iodotyramine derivatives (3,5-diiodotyramines and tyrosine derivatives) with antitumoral activity were recently isolated from the aqueous extract of ascidian Didemnum rubeum. 2 Moreover, iodine derivative alkaloid 3,5-diiodo-4-methoxyphenetylamine and their respective urea analogs were isolated from the tunicates Lissoclinum patella and Didemnum ternatanum.Both derivatives were active against C. albicans strains and mouse lymphocytic leukemia L1210 cell line at concentrations of half maximal inhibitory concentration (IC 50 ) 20 μg mL -1 . 3Other active diiodotyramines isolated from marine organisms are turbotoxins A and B both isolated from gastropod Turbo marmorata.These diiodotyramines exhibited an acute toxicity against ddY mice, with an dosage required to kill 99% of the test population (LD 99 ) of 1.0 and 4.0 mg kg -1 , respectively. 4However, their synthetic analogs were 100fold less toxic when the quaternary ammonium group was changed by a tertiary ammonium group. 5Due to iodinated alkaloid metabolites, usually isolated as tyrosine and tyramine derivatives, diiodotyramides and diiodotyrosines show broad biological activities, but are not very common in nature, therefore, chemical synthesis of these compounds emerge as an opportunity to potentiate their biological activity through structural changes and develop new active agents that may overcome the problem of parasite resistance against current drugs.For this reason, the potential of synthetic compounds based on diiodotyramides aromatic esters, derivatives of marine natural products, was evaluated

Anti-Parasite Activity of Novel 3,5-Diiodophenethyl-benzamides
Manuel Pastrana Restrepo, a Verónica Surmay Surmay, a Elkin Galeano Jaramillo* ,a and Sara Robledo Restrepo b in this study for cytotoxicity, but also for anti-protozoan and hemolysis activities.

Chemistry
The aromatic iodo-tyramides syntheses were made in two stages (Scheme 1).The first is the synthesis of compounds C and D, and later coupling with different aromatic acids to afford the respective amides.
The protection of the amino group in the tyramine was done in basic media with Boc 2 C, 6 and the diiodination of the aromatic ring with N-iodosuccinimide (NIS), 7 getting a di-halogenated compound in positions 3 and 5, obtaining compound A with a 70% yield for two steps. 1 H nuclear magnetic resonance (NMR) analysis of compound A shows a singlet at 7.50 ppm for two protons, which corresponds to hydrogen at 2, which confirm the diiodination at the ortho position of the phenyl ring.The singlet at 1.44 ppm for nine protons corresponds to t-butyl group for the carbamate moiety.Compound B was produced by the reaction of A with methyl iodide in basic media, 8 with 80% yield. 1 H NMR spectra of compound B shows a singlet at 3.84 ppm for three protons, corresponding to O-methyl group in the aromatic ring.
Acid hydrolysis of compounds A and B 9 produce the primary amines in compounds C and D. The loss of the signal at 1.44 ppm in both compounds shows the hydrolysis of the carbamate moiety.
The amide syntheses were done by the coupling of C and D with different aromatic acids using N,N'-diisopropyl-carbodiimide (DIC) in dimethylformamide (DMF) catalyzed with 4-dimethylaminopyridine (DMAP), 10 in yields ranging 45-50%. 1 H NMR analysis of the amides shows the respective singlet for two protons in the downfield area corresponding to hydrogens at ortho position in the iodo-tyramine moiety.Besides, the signals corresponding to the aromatic protons in the aromatic acids scaffold appear in the 6-8 ppm region.p-Substituted aromatic acids show these signals as two doublets confirming the symmetry of the aromatic ring.The presence of methyl and O-methyl groups in the aromatic rings was confirmed by the singlets at 2.34 and 3.70 ppm.The 1 H-1 H correlation spectroscopy (COSY) analysis shows the respective couplings of protons in the aromatic acid and two methylene group in the iodotyramine chain.The molecular formula of the respective amides was confirmed by high-resolution mass spectra (HRMS) analysis.In this way it was possible to determine unambiguously the chemical structure of the new derivatives synthesized.

Biological activities
The effect of the eight synthesized amides on cell growth and viability on human U-937 macrophages was assessed.In addition, the anti-parasite activity on L. panamensis and T. cruzi intracellular amastigotes and total sexual forms of P. falciparum was tested, according to the ability of compounds to reduce the amount of parasite in culture.The hemolytic assay was done on human red blood cells (RBC) obtained from a blood bank.Results are summarized in Table 1.
The anti-parasite activities were measured by determining the effective concentration 50 (EC 50 ).The anti-leishmanial assay showed that compounds 2 and 7 were the most active with EC 50 of 17.9 and 17.5 μg mL -1 , respectively.Compounds 1, 3, 5, 6 and 8 had moderate activity with EC 50 values between 25 to 50 μg mL -1 .The compound with the highest selectivity index (SI) for L. panamnesis was compound 1 with a value of 6.97 μg mL -1 .It is the safest of the iodotyramides derivatives.Compound 1 was more than 8-fold less cytotoxic than amphotericin B. Anti-trypanosomal assays showed that compounds 6 and 7 were the most active with EC 50 of 23.75 and 6.19 μg mL -1 , respectively.Moreover, compound 7 was more active than benznidazole.In turn, compounds 2, 5 and 8 were moderately active with values ranging from 25 to 50 μg mL -1 .Compound 1 was the most selective against T. cruzi with an SI of 5.2.
Finally, antiplasmodial activity showed that all derivatives had a high activity with EC 50 values < 25 μg mL -1 , except compound 1.No compound was more active than chloroquine.Compound 8 had the highest SI with a value of 10.48.Compounds 4, 5, 6 and 7 were also selective with SI values > 8.The p-O-Me compounds were most active than their more polar analogs against L. panamnesis and T. cruzi.The core A, 3,5-diiodo-p-methoxybenzyl, is correlated with a higher antiparasitic activity against the three parasites tested.Those derivatives showed the best selectivity index against P. falciparum.No compound was hemolytic on human RBC.

Conclusions
In this work it is reported the complete synthesis of N-(3,5-diiodo-4-methoxyphenethyl)-benzamide, an iodotyramide isolated for the first time from ascidian Didemnun rubeum and seven correlated new tyramides with different substitution in para-position of the aromatic acid moiety.Antiparasitic activity against T. cruzi, L. panamensis and P. falciparum and also cytotoxicity and hemolysis assays were reported.All compounds had LC 50 < 100 μg mL -1 , except compound 1, making them potentially cytotoxic for U-937 macrophages.Compounds 2-8 had high activity against P. falciparum, where compound 6 was the most promissory compound.Anti-trypanosomal assays showed that compounds 6 and 7 had the higher activity, where 7 was the most active against T. cruzi.In turn, anti-leishmanial assays showed that compounds 2 and 7 were the most active.No compound produced hemolysis of RBC.Based on these in vitro biological activities, further in vivo studies are necessary to validate anti-parasite activity observed of compounds 2, 6 and 7.

General remarks
All laboratory reagents were analytical grade (Sigma-Aldrich ® ).Chromatography column and HPLC solvents were liquid chromatography grade (Merck ® ).Compound purifications were made by column chromatography using Merck ® silica gel 60 and mixtures of hexane and ethyl acetate.Purifications of biologically tested compounds were made by HPLC Agilent 1200 with DAD detector (254, 280 and 366 nm) and Eclipse XDB-C18 column using a mixture of MeOH and H 2 O 0.1% formic acid as the mobile phase. 1 H and 13 C NMR were recorded on Bruker Ascend III HD (600 and 125 MHz) with 5 mm cryoprobe TCI, using CDCl 3 and dimethyl sulfoxide (DMSO-d 6 ) as deuterated solvents.Signals were assigned using two-dimensional heteronuclear correlations (COSY (correlation spectroscopy) and HSQC (heteronuclear single quantum correlation)).HRMS were recorded using electrospray ionization (ESI-MS) in a UPLC-Q-Tof, reference Xevo-XS-Qtof, Waters.The drying and cone gas was nitrogen set to flow rates of 300 and 30 L h -1 , respectively.Methanol samples solutions (1 × 10 -5 mol L -1 ) were directly introduced into the ESI spectrometer at a flow rate of 10 μL min -1 .A capillary voltage 3.5 kV was used in the positive scan mode, and the cone voltage set to Uc = 10 V.

General procedure for the synthesis of aromatic tyramides (1-8)
Compound C or D (0.15 mmol), DIC (1.28 mmol, 200 μL), DMAP (0.5 mmol, 61 mg), aromatic acid (benzoic acid, 4-methyl benzoic acid, 4-methoxy benzoic acid and 4-nitrobenzoic acid) (0.5 mmol) and DMF (10 mL) were placed into a round bottom flask equipped with a magnetic stirring bar.The mixture was stirred at room temperature for 36 h.The crude reaction mixture was evaporated under reduced pressure and the residue was purified by column chromatography over silica gel eluting with hexane-ethyl acetate (4:1 to 1:1) to obtain the aromatic iodotyramides.

In vitro cytotoxicity
Cytotoxicity of the compounds was evaluated on human U-937 macrophages (ATCC CRL-1593.2) in the exponential growth phase and adjusted at 1 × 10 5 cells mL -1 in RPMI-1640 enriched with 10% fetal bovine serum (FBS).100 mL of cells were dispensed in each well of a 96-well microplate and 100 mL of 200, 50, 12.5 or 3.125 μg mL -1 concentration of each compound were added.Cells exposed to compounds or standard drugs were incubated for 72 h at 37 °C and 5% of CO 2 .Cytotoxic activity of each compound was determined according to the effect on the cell viability by the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) micro enzymatic method in which MTT is reduced to a purple product named formazan by mitochondrial enzyme succinate dehydrogenase.Thus, 10 μL well -1 were added to each well, and plates were incubated at 37 °C, 5% CO 2 for 3 h.The reaction was stopped by adding 100 μL well -1 of isopropanol with 50 and 10% of sodium dodecyl sulfate (SDS).The concentration of formazan was spectrophotometrically determined at 570 nm (Varioskan, Thermo) and the intensity of the color (absorbance) was registered as optical densities (O.D.).Cells exposed to control drugs chloroquine, amphotericin B, benznidazole, and doxorubicin were used as positive control for toxicity, while cell incubated in RPMI-1640 medium alone were used as negative control.Non-specific absorbance was corrected by subtracting absorbance (O.D.) of the blank.Determinations were done by triplicate in at least two independent experiments. 11

Hemolytic activity
The ability to induce hemolysis was specifically evaluated to compounds which showed antiplasmodial activity following the method of cytotoxicity by spectrophotometry on 96-well plates.Briefly, 500 μL of human red blood cells (huRBC), adjusted to 5% hematocrit in RPMI-1640 medium, were placed into each well of a 24-well plate and subsequently exposed to 200 μg mL -1 of compounds 1-8.Detection of free hemoglobin, after 48 h of incubation at 37 °C, was the evidence that the compound induced hemolysis.The concentration of free hemoglobin was spectrophotometrically read at 542 nm (Varioskan, Thermo) and O.D. were registered.Non-specific absorbance was corrected by subtracting absorbance of the blank.Determinations were done by triplicate in at least two independent experiments. 12 vitro anti-leishmanial activity Anti-leishmanial activity of iodotyramides was determined according to the ability of the compound to reduce infection by L. panamensis parasites. 13Briefly, U-937 human cells at a density of 3 × 10 5 cells mL -1 in RPMI 1640 and 0.1 μg mL -1 of phorbol-12-myristate-13-acetate (PMA) were dispensed on 24-wells microplate and then infected with stationary phase growing L. panamensis promastigotes (MHOM/CO/87/UA140-E-GFP strain) in a 15:1 parasites per cell ratio.Plates were incubated at 34 °C and 5% CO 2 for 3 h and then cells were washed twice with phosphate buffer solution (PBS) to eliminate not internalized parasites.Fresh RPMI-1640 was added to each well (1 mL) and plates were incubated again to complete infection.After 24 h of infection, the RPMI-1640 medium was replaced by fresh culture medium containing each compound at four serial dilutions (50, 12.5, 3.125 and 0.78 μg mL -1 ) and plates were incubated at 37 °C and 5% CO 2 for 72 h; then, cells were removed from the bottom plate with a 100 μL trypsin/ethylenediaminetetracetic acid (EDTA) solution.The cells were centrifuged at 1100 rpm during 10 min at 4 °C, the supernatant was discarded and cells were washed with 1 mL of cold PBS and centrifuged at 1100 rpm for 10 min at 4 °C.Cells were washed two times employing PBS, as previously, and after the last wash, the supernatant was discarded and cells were suspended in 500 μL of PBS.Cells were analyzed employing a flow cytometer (Cytomics FC 500MPL) reading at 488 nm (exciting) and 525 nm (emitting) over an argon laser and counting 10,000 events.Infected cells were determined according to the events for green fluorescence (parasites).Infected cells exposed to amphotericin B (standard antileishmanial drug) were used as control for anti-leishmanial activity (positive control), while infected cells incubated in absence of any compound or drug were used as control for infection (negative control).Nonspecific fluorescence was corrected by subtracting fluorescence of unstained cells.All determinations were performed by triplicate in at least two independent experiments. 11,13 vitro anti-trypanosomal activity To test the effectiveness of compounds, 25,000 U-937 human macrophages/100 µL RPMI-1640 enriched with 10% FBS and 10 ng PMA were placed in each well of 96-wells cell culture plate.Cells were then infected with epimastigotes (24 h of growing) of T. cruzi Tulahuen strain transfected with β-gal gene (donated by Dr F. S. Buckner, University of Washington) in 5:1, parasites:cell ratio.After infection, 100 µL of each compound at 50, 12.5, 3.125 or 0.78 μg mL -1 were added to each well and plates were incubated during 72 h at 37 ºC, 5% CO 2 .Finally, the effect of each compound and each concentration on the viability of intracellular parasites was determined by measuring β-gal activity by colorimetric method after adding 100 µM CPRG and 0.1% nonidet P-40.After 3 h of incubation at room temperature plates were read at 570 nm in a spectrophotometer (Varioskan, Thermo) and absorbance was recorded as O.D. Anti-trypanosomal activity of benznidazole was used as positive control, while RPMI-1640 medium was used as negative control.Non-specific absorbance was corrected by subtracting O.D. of the blank.Determinations were done by triplicate in at least two independent experiments. 14 vitro anti-plasmodial activity Asynchronized P. falciparum 3D7 strain cultures were adjusted to 0.5% parasitemia and 1% hematocrit in RPMI medium enriched with 3% lipid-rich bovine serum albumin-Albumax II.Then, 100 μL of parasite suspension was dispensed into each well of 96-wells cell culture plate and subsequently exposed to 100 μL of each compound at 100, 25, 6.25 or 1.56 μg mL -1 .Plates were incubated for 48 h at 37 °C in N 2 (90%), CO 2 (5%) and O 2 (5%) atmosphere.After incubation, parasites were harvested and subjected to three 20 min freeze-thaw cycles.Meanwhile, 100 μL of Malstat reagent (400 μL Triton X-100 in 80 mL deionized water, 4 g L -1 lactate, 1.32 g Tris buffer and 0.022 g acetylpyridine adenine dinucleotide in 200 mL deionized water; pH 9.0) and 25 mL of NBT/PES solution (0.16 g nitroblue tetrazolium salt and 0.08 g phenazine ethosulphate in 100 mL deionized water) were added to each well of a second 96-well plate.After freeze-thaw cycles, culture in each of the wells of the first plate was resuspended by pipetting and 15 μL of each well was taken and added to the corresponding well of the second plate (containing Malstat and NBT/PES reagents).After an hour of incubation in the dark, color development of the LDH (lactate dehydrogenase) reaction was read in a spectrofluorometer (Varioskan, Thermo) at 650 nm.The intensity of color in each experimental condition was registered as fluorescent units (F.U.).Non-specific fluorescence was corrected by subtracting F.U. of the blank.Determinations were done by triplicate in at least two independent experiments.Chloroquine (CQ) was used as positive control and culture medium was used as negative control. 15

Statistical analysis
Cytotoxicity of iodotyramides was tested in comparison to doxorubicin, amphotericin B, benznidazole, chloroquine and RPMI-1640 culture medium.The results were expressed as LC 50 that corresponds to the concentration necessary to eliminate 50% of cells and calculated by Probit analysis.Cell growth inhibition was calculated by equation 1: (1) The degree of toxicity was graded according to the LC 50 value using the following scale: high cytotoxicity: LC 50 < 100 μg mL -1 ; moderate cytotoxicity: LC 50 100-200 μg mL -1 range and potentially non-cytotoxicity: LC 50 > 200 μg mL -1 .
Anti-leishmanial activity was determined according to the reduction of the median fluorescence intensity (MFI) of infected cells obtained for each experimental condition by the cytometer.The parasite amount was calculated by equation 2, where the MFI in infected cells in the control well corresponds to 100% of infection.
(2) Anti-plasmodial activity of each evaluated compound was evidenced by the reduction of growing parasites calculated according to equation 3: (3) Anti-trypanosomal activity was calculated by reduction of infection (viable parasites) in each tested concentration.For this, reduction of the number of parasites was calculated with equation 4: (4) Percentages of reduction of parasites data were used to calculate the EC 50 using the linear regression model Probit. 16nti-leishmanial, anti-plasmodial and antitrypanosomal activities were graded as high, moderate or low according to the EC 50 values, as follow: high activity when the EC 50 < 25 μg mL -1 ; moderate activity EC 50 ranging between 25-50 μg mL -1 and low activity when the EC 50 > 50 μg mL -1 .SI was calculated by dividing the cytotoxic activity and the anti-leishmanial, anti-trypanosomal and antiplasmodial activity using the following formula: SI = LC 50 /EC 50 .