Synthetic Niobium Oxyhydroxide as a Bifunctional Catalyst for Production of Ethers and Allyl Alcohol from Waste Glycerol

A synthetic niobia was modified by treatment with hydrogen peroxide to be used as a catalyst for converting glycerol into petrochemical compounds. The catalytic properties of niobium oxyhydroxide were obtained by generating acidic and oxygenated groups (peroxo groups) on the solid surface. Compounds like ethers and allyl alcohol were obtained. A very active H2O2-modified catalyst showed high activity for the oxidation and dehydration of 91% of glycerol conversion using H2O2 and 46% of allyl alcohol selectivity in volatile phase. The product was analyzed by gas chromatography-mass spectrometry (GC-MS), thermogravimetry-mass spectrometry (TGA-MS) and nuclear magnetic ressonance (NMR). These results strongly suggest that the reactions involve acid and oxidizing species generated after the reaction of niobium with H2O2. H and C multidimensional NMR spectroscopy confirmed the results obtained with GC-MS and showed the production of several compounds after niobia treatment.


Introduction
Brazil is a country that stands out for its great capacity to produce renewable energy, starting with the Proálcool program for the use of ethanol as a fuel alternative to gasoline. 1 The country currently has a national program for the gradual inclusion of biodiesel in diesel fuel 2 (2% at the beginning of the program, 5% from 2010 and 7% nowadays). 3Biodiesel appears as a promising energy source that would cause less environmental impact, 4 especially by reducing CO 2 emissions. 5he most appropriate way to yield such diesel fuel is by converting the triacylglyceride structure of vegetable oils or animal fats into fatty acid mono-alkyl esters standardized as biodiesel when meeting the requirements of ASTM D 6751 and Euro norm EN 14214. 6The production of biodiesel by transesterification of triacylglycerides yields glycerol as a byproduct.The large amounts thus produced have turned glycerol from a specialty chemical into a commodity.Unfortunately, the production of this fuel has certain limitations, such as the generation of large amounts of glycerol as a byproduct.Although glycerol is widely used as a raw material in various industrial applications, the material is nevertheless produced in much larger amounts than needed. 6However, to be used as a renewable raw material to replace petrochemicals in the production of important or promising chemical intermediates like epichlorohydrine (ECH), propylene glycol (PG), acrylic acid or 1,3-propanediol, 7,8 the forecasted by-product volumes would be largely insufficient.Currently, the Brazilian market consumes approximately 40,000 tons year -1 of glycerol, however, the production with the introduction of B2 (2% of biodiesel in regular diesel) in 2008 was 80,000 tons year -1 of glycerol. 9In 2014, Brazil produced 390,000 tons of crude glycerin from biodiesel. 10This increase shows that the viability of the biodiesel program is directly related to the ability to produce economically feasible materials from the residual glycerol.
2][13][14] Thus, US patent 5,387,720 15 showed that glycerol undergoes dehydration in liquid or vapor phase using supported acid catalysts.The production of fuel Vol. 28, No. 11, 2017   additives has also been studied, 16 but the drastic conditions and the difficult separation of the homogeneous catalyst from the reaction medium make the process expensive.Some studies on glycerol conversion have involved heterogeneous catalysis, mainly using materials with acidic properties such as zeolites, aluminosilicates and niobium oxide. 17Other interesting works have studied the conversion of glycerol into polyglycerols and polyglycerol esters that could be used for the production of surfactants, lubricants, cosmetics or food additives. 12][27][28] A niobium oxyhydroxide was synthesized and modified by hydrogen peroxide treatment to generate a bifunctional catalyst.The catalysts were tested for the simultaneous dehydration/oxidation of glycerol to obtain compounds that could be employed as bio-additives for fuels.

Synthesis and characterizations
Niobium oxyhydroxide was prepared by treating NH 4 [NbO(C 2 O 4 ) 2 (H 2 O)]•(H 2 O) n with NaOH (50 mL, 1 mol L -1 ), followed by heating at 60 °C for 72 h.This material was used as a precursor (pure Nb 2 O 5 ) for the preparation of treated niobia (Nb 2 O 5 /H 2 O 2 ), obtained by treating pure niobia (300 mg) with hydrogen peroxide (30% v v -1 , 4 mL) in water (80 mL) for 30 min.The yellow solid was then filtered, washed with distilled water and dried at 60 °C for 12 h.
Fourier transform infrared (FTIR) measurements of the catalyst were carried out with a Shimadzu Prestige 21 spectrophotometer equipped with an attenuated total reflectance (ATR) accessory.The spectra of the samples were obtained in the region from 4000 to 240 cm -1 using a CsI crystal and 150 signal-averaged scans.The textural properties were investigated by nitrogen physisorption at 77 K to determine the specific surface area and total pore volume using a Quantachrome model Autosorb-1.The samples were previously outgassed at 200 °C and the specific surface area was obtained using the Brunauer-Emmett-Teller (BET) method.The experimental setup thermogravimetry-mass spectrometry (TGA-MS) consists of a thermogravimetric analyzer (STA 409 PC/PG, Netzsch Instruments, Germany) coupled with a quadrupole mass spectrometer (QMG 422, Pfeiffer Vacuum Technology, Germany).In the thermogravimetric analyzer, high purity nitrogen (99.9995%) at a flow rate of 60 mL min -1 was used as the carrier gas to provide an inert atmosphere and to remove the gases.About 8 mg of sample (particle size small than 60 μm) were pyrolyzed in TGA from room temperature to 900 °C with a heating rate of 20 °C min -1 .The released gases were purged immediately to the mass spectrometer to obtain the evolution curves.The transfer line and gas cell were pre-heated to 200 °C in order to avoid cold spots and to prevent the condensation of semi-volatile products.The mass spectrometer was operated in electron ionization mode with 70 eV of electron energy.The mass range m/z 10-300 was scanned with scan rate of 5 scans s -1 .

Catalytic tests
Glycerol conversion was performed using H 2 O 2 (30% v v -1 ) as the oxidizing agent.The catalytic tests were performed at 250 °C, using 20 mL concentrated glycerol and 50 mg catalyst in the presence of H 2 O 2 (1.0 mL) in a batch reactor for 1, 3 and 6 h.Two distinct liquid phases were collected from the reaction.The first one (the heavier phase) was collected directly from the reactor after a given time and reaction conditions, called the condensed phase (CP sample).The other liquid phase (rich in low molecular mass compounds) was collected through a condenser attached to the reactor, called the volatile phase (VP) (Figure 1).The products of the reaction were analyzed by gas chromatography-mass spectrometry (GC-MS) (Agilent Instruments, Santa Clara, CA, USA).
The glycerol conversion was determined by the ratio of the glycerol peak integration value and the total ion content (TIC), obtained before and after the reaction with the catalyst.The GC-MS parameters for the analysis of the condensed phase were as follows: injector temperature at 250 °C, injection volume of 0.2 mL, flow rate of 1.4 mL min -1 and a HP-5 (5% polimetilfenilsiloxane) column.A heating curve of 80 °C for 5 min, followed by 3 °C min -1 until 250 °C, was used.In order to achieve a better separation of the compounds, a different method was developed for the analysis of the volatile phase.
The oven temperature was held at 50 °C for 5 min and then increased to 250 °C at 3 °C min -1 .The carrier gas (He) was used at a flow rate of 1.2 mL min -1 .A series of alkanes (C 8 -C 22 ) was analyzed using the same method to establish retention indices in order to tentatively identify the compounds by comparison with the literature (either from the National Institute of Standards and Technology (NIST) 2008 library or from other literature sources).When using the NIST library, only probabilities greater than 90% were considered.
The NMR (nuclear magnetic ressonance) samples of the aliquots were prepared in methanol-d 4 .These aliquots were collected after 3 and 6 h (CP-3h and CP-6h, respectively) for the condensed phase and after 6 h for the volatile phase (VP-6h).All the NMR spectra were recorded at 300 K on a Bruker Avance DRX 400 MHz spectrometer operating at 9.4 T. A 5 mm inverse detection gradient probe was used for the acquisition of the 1 H, 1 H- 13 C HSQC (heteronuclear single-quantum correlation) and HMBC (heteronuclear multiple bond correlation) spectra, while a 13 C-1 H direct detection 5 mm dual probe was employed for the acquisition of the 1 H-decoupled 13 C and DEPT-135 (distortionless enhancement by polarization transfer) spectra.The products of the reaction are shown in Figure 2.

Catalytic tests
The analyses of the condensed fraction showed glycerol conversion rates of 18, 57 and 91% after 1, 3 and 6 h, respectively.The catalytic activity exhibited by the materials in this work was comparable with others reported in the literature.Sánchez et al. 29 obtained a glycerol conversion rate of 78% in gas phase with GHSV (gas hourly space velocity) of 1190 h -1 at 340 °C using γ-alumina/Fe catalyst and allyl alcohol yield was 4.3%.Different catalysts with alkali metals, including Na, K, Rb, and Cs, were supported on ZrO 2 -FeO x and all of them gave impressively higher allyl alcohol yields and suppressed glycerol dehydration due to the reduced catalyst acidic property. 30ith achieved K-supported ZrO 2 -FeO x (K/ZrO 2 -FeO X ), the allyl alcohol yield remarkably increased up to 27 mol% C at the K content of 3-5 mol%.
The reaction containing only H 2 O 2 (without the catalyst) showed low activity, exhibiting a conversion of approximately 11%.In the absence of hydrogen peroxide (catalyst only), the conversion rate was 45.9% (at 6 h of reaction), showing the importance of the oxidizing agent to regenerate the catalyst in situ.After 6 h, almost all of the glycerol was converted into new products, mainly diglycerol and ethers in the condensed phase and allyl alcohol in the volatile phase.This work presents, in volatile phase, the selectivity of allyl alcohol (46% at 6 h of reaction) and (CP) ether compounds (selectivity 25%) from glycerol using a niobium oxyhydroxide compound directly as a catalyst without an active phase such as a noble metal.
To identify the molecules formed in the condensed and volatile phases, the samples were analyzed by GC-MS (Figures 3 and 4, respectively).The details in Figure 3 show the difference in the glycerol conversion and the formation of ethers.After 3 h, ethers as di-and triglycerols are preferably formed.These compounds appear close to 28 min in the GC-MS trace and were confirmed by Kovats retention index.The triglycerols were obtained in low amounts as shown in the chromatography analyses.This difference may be due to the difficulty of volatilizing these molecules.
The compounds with retention times between 16 and 19 min (the major compounds) were determined to be 6-carbon cyclic ethers.The mechanism for the formation of these cyclic ethers was shown by Barrault et al. 31 Another way to generate these cyclic compounds is by the dimerization of 3-hydroxypropionaldehyde proposed by Suprun et al. 32 and Oliveira et al. 33 using electrospray analysis, and presented a predominance of di-and triglycerols in the condensed phase from the conversion of glycerol with catalyst based on nioubium compounds.
For the first time, the formation of ketones and allyl alcohol from glycerol is reported along with its identification using NMR to corroborate the analysis by chromatography.Along with allyl alcohol, acetone and butanedione were also obtained, the latter being 10% of the total area in the volatile phase analysis.In fact, the results show that the reaction with H 2 O 2 -modified niobium oxyhydroxide was more effective and more selective than the reaction using unmodified niobium oxyhydroxide (data not shown) because a higher conversion was obtained.We attributed this phenomenon to the surface oxidizing species (i.e., peroxy groups formed with the H 2 O 2 treatment of the catalyst).Peroxo and acidic groups are shown to be the active species in the conversion of glycerol promoting the formation of ethers and ketones. 23,34The proposed structures of the compounds obtained in the condensed phase are displayed in Figure 5.
Chieregato et al. 35 showed that W/V/Nb catalytic systems produce in minor amounts of acetic acid, allyl alcohol, acetone and hydroxyacetone from glycerol reactions.In another study, the authors report the formation of cyclic ethers from glycerol reactions using acid catalysts, 36 however, there is the possibility of occuring a oxidative cleavage of glycerol and triggering the formation of by-products that usually is leaded under strictly reducing conditions.The mechanism of allyl   alcohol formation is well reported via hydrogen transfer through the potential donor in the reaction medium. 37he conversion of glycerol to allyl alcohol and cyclic ethers from the reaction of the mixture of niobium and vanadium oxides with hydrogen peroxide has been described. 28Oliveira et al. 28 showed a glycerol oxidative cleavage under oxidizing conditions producing formic acid, which is able to mediate the formation of both allyl alcohol and cyclic ethers.For this, the formic acid acts as intermediate product reacting with unreacted glycerol yielding 4-hydroxymethyl-1,3-dioxolane.The formic acid may react immediately when is produced because it is not observed in the GC-MS and neither 1 H NMR analyses.
The 13 C NMR spectra obtained from the samples CP-3h, CP-6h and VP-6h are presented in Figure 6.In these spectra, relatively intense signals were observed at 63.97 and 73.53 ppm, which correspond to the 13 C resonances of unreacted glycerol.Nevertheless, many other resonances were observed in the three spectra, suggesting that glycerol was converted into many other species.By comparing the spectrum of CP-6h to that of CP-3h, the glycerol signal intensities were observed to decrease compared to other signals, indicating that higher degrees of conversion require longer reaction times.A significantly higher number of NMR signals observed in the 13 C NMR spectrum of CP-6h confirmed the formation of several compounds and a much greater consumption of glycerol.When the 13 C NMR spectra obtained from the volatile and condensed phase samples were compared to each other (CP-6h and VP-6h), many different resonance signals indicated that these two phases were composed of distinct compounds.It suggests that a detailed analysis of these samples is required to determine their compositions.
Among these substances, many useful high-value chemicals were expected to be found.The analyses of the NMR spectra unequivocally confirmed all of the compounds identified by GC-MS in both the condensed and volatile phases.Other than acetone, butanone, 2,3-butadione, propyl alcohol and allyl alcohol, other compounds such as acrolein and dimethyldioxane were identified in the NMR spectra of the volatile phase (see the SI section).These assignments were performed by the simultaneous analysis of 13 C, DEPT-135, edited 1 H- 13 C HSQC and HMBC spectra.Expansions of the 1 H- 13 C HSQC and HMBC contour plots with the correlations of the acrolein highlighted resonances are presented in Figures 7 and 8.
In the 1 H- 13 C HSQC spectrum, the negative phase signals represent correlations between the diastereotopichydrogens and the respective carbons of the methylene group, whereas the positive phase signals represent the correlation between the hydrogen and the carbon of acrolein.In the 1 H-13 C HMBC contour plot, the resonances of the olefinic groups were connected to each other, as well as to the carbon and hydrogen of the formyl group, which possessed signals at 198.9 and 9.47 ppm, respectively.
As mentioned above, the high number of constituents observed in both the volatile and condensed phases hampered the detailed analysis of these spectra.Therefore, we partially fractionated these products and then analyzed them with NMR to determine the identities of the possible compounds with potential industrial applications.
A glycerol standard (Figure 9a) and the products formed after 6 h (Figure 9b) were studied by TGA-MS.In the TGA-MS analysis of the glycerol standard, only ions at m/z 18 and 28 were observed at high intensity.These ions may be due to water or carbon monoxide, which could be created by the degradation of glycerol.
From the mass spectrometry data of allyl alcohol obtained in the volatile fraction, ions at m/z 31, 39 and 57 were observed, as typically observed for this compound.Interestingly, these ions do not appear in the glycerol standard or in the products, indicating that allyl alcohol does not come from pyrolysis of glycerol or its products, but as a result of the catalytic process.
Figure 10 presents ion fragments of products from glycerol.These ion fragments are similar to the ones reported by Dass 39

Characterization of the materials
The physical characteristics of the niobia catalysts The FTIR spectra of the materials are shown in Figure 11.The absorption bands at approximately ca. 3412 and ca.3140 cm -1 are assigned to the O-H stretching frequencies in different regions of the catalyst structure (i.e., the surface and bulk structures, respectively).
To obtain more detailed information about the hydroxyl groups involved in the formation of peroxy groups, a deconvolution approach using a Gaussian adjustment was performed on the spectra of Figure 12.
Due to band overlapping, this procedure improved the resolution of each individual band.The obtained curve fit (Figure 12) showed the presence of five components, but only the two main, with higher frequencies, were investigated.The index value (I s ) calculated by the ratio of the band areas at 3412 and 3140 cm -1 was used to express the relative quantity of the different hydroxyl groups.
After reacting niobium oxyhydroxide with hydrogen peroxide, the I s value decreased from 0.49 (pure Nb 2 O 5 ) to 0.43 (Nb 2 O 5 /H 2 O 2 ), indicating a decrease in the amount of surface hydroxyl groups in relation to the bulk hydroxyl groups.As observed in the FTIR spectra of niobium oxyhydroxide after H 2 O 2 -treatment (Figures 12a and 12b, respectively), the band at 3140 cm -1 becomes more intense than the others present in this region compared to the spectrum for niobium oxyhydroxide before H 2 O 2 -treatment.This result suggests that the surface hydroxyl groups are preferentially involved in the formation of peroxo groups.In addition, the formation of peroxo groups is evidenced in the FTIR spectrum of Nb 2 O 5 /H 2 O 2 by the appearance of shoulders in the band at ca. 537 cm -1 , assigned to the O-O asymmetrical stretching bonded to the metal (Nb-O 2 ), and at 868 cm -1 associated with the stretching vibration of the peroxo group (O-O), as highlighted in Figure 11.

Conclusions
In this work, new potentially useful organic compounds were obtained from waste glycerol that may be used as fuel additives or in other possible industrial applications.These compounds were formed by the reaction of waste glycerol with peroxo groups generated on the surface.These modified catalysts showed great potential for the conversion of glycerol using H 2 O 2 as an oxidant.analyses were also used to identify products from the conversion of glycerol, and these results corroborate the chromatograph analyses, which showed the formation of mainly ethers in the condensed phase and ketones and allyl alcohol in the volatile fraction.The results presented here    Results obtained from TGA-MS analyses showed a different profile of compounds compared to standard glycerol, confirming the activity of the catalyst.NMR show that useful petrochemical products can be obtained using synthetic niobium oxyhydroxide as a catalyst by modifying the metal surface to generate both oxidizing and acidic groups.

Figure 2 .
Figure 2. Pictures of the volatile and liquid phase products.

Figure 3 .
Figure 3. Chromatogram of the condensed phase obtained after 3 and 6 h of reaction (50 mg of niobium oxyhydroxide, 1 mL de H 2 O 2 and 250 o C).

Figure 4 .
Figure 4. Profile of volatile products obtained after 6 h of reaction (50 mg of niobium oxyhydroxide, 1 mL de H 2 O 2 and 250 o C).

Figure 5 .
Figure 5. Proposed structures of compounds obtained from the reaction.
Many resonances were observed between 58 and 85 ppm, indicating the presence of many ether compounds in both the volatile and condensed phases (C-O resonances).The presence of these signals along with resonances between 171 and 176 ppm (-C(O)O-resonances) in the CP-6h 13 C NMR spectrum indicates the conversion of glycerol into esters in the condensed phase.Interestingly, in the VP-6h 13 C NMR spectrum, many signals of nonhydrogenated carbons (confirmed by DEPT-135 spectrum, see the Supplementary Information (SI) section) were observed between 198 and 217 ppm, hereby indicating the presence of ketones in the volatile phase.These observations demonstrate that the two phases are composed of different classes of carbonyl compounds.Whereas the condensed phase is mainly composed of esters, ketones are most common in the volatile phase.It is also worth mentioning that many resonances were seen between 100 and 165 ppm, demonstrating the presence of unsaturated compounds (either olefins or aromatic compounds) in the volatile phase.In particular, the intense signals observed at 115.8 and 138.1 ppm confirm a high allyl alcohol yield.
in mass spectrometry studies.In turn, the metastable ions of protonated glycerol fragment principally via the loss of a water molecule producing extensive fragmentation from m/z 75.The ions of m/z 45, 44 and 43 involve the losses of elements of CH 2 O, CH 3 O and CH 4 O from the ion of m/z 75.The glycerol protonated ion undergoes a loss of a CH 3 OH molecule to yield m/z 61, that can loss of a water molecule to yield ion m/z 43.

Figure 7 .
Figure 7. Partial 1 H-13 C HSQC contour plot (400 MHz, CD 3 OD-d 4 ) of VP-6h at 300 K.The positive phase is presented in black, and the negative phase in turquoise.The acrolein correlations are assigned.

Figure 9 .
Figure 9. Thermogravimetric analysis coupled with mass spectrometry of the (a) glycerol standard and (b) products obtained after 6 h.