08Jan 2019

EVALUATION OF ANTIBIOFILM ACTIVITY OF THE LATEX EXTRACTED FROM HIMATANTHUS DRASTICUS (MART.) PLUMEL (JANAGUBA).

  • Laboratorio de Biologia Molecular, Departamento de Bioquimica, Universidade Federal de Pernambuco, Pernambuco, Brasil.
  • Laboratorio de Microbiologia e Imunologia Aplicada, Universidade Federal de Pernambuco, Pernambuco, Brasil.
  • Laboratorio de Microbiologia de Alimentos, Centro Academico de Vitoria, Universidade Federal de Pernambuco, Pernambuco, Brasil.
  • Departamento de Antibi?ticos, Universidade Federal de Pernambuco, Pernambuco, Brasil.
  • Nucleo de Bioprospeccao da Caatinga, Instituto Nacional do Semiarido, Paraiba, Brasil.
  • Laboratorio de Nanotecnologia, Biotecnologia e Cultura de Celulas (NANOBIOCEL), Centro Academico de Vitoria, Universidade Federal de Pernambuco, Pernambuco, Brasil.
  • Espaco Farmacia Viva, Centro Academico de Vitoria, Universidade Federal de Pernambuco, Pernambuco, Brasil.
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The secondary metabolites present in plants often have antibiofilmactivity, this property attributed to plant compounds is of great importance, given the formation of biofilms in biomaterials, which further boosts the search for natural substances capable of minimizing this type of formation. Himatanthusdrasticus(Mart.) Plumel is a tree species popularly known as janaguba, this is found in areas of caatinga, cerrado, rupestrian fields and rainforest. Community empirical knowledge attributes to this plant species therapeutic action against tumors, hemorrhoids, respiratory tract infections, digestive tract and urogenital tract. Considering the reduced number of scientific findings on the biological activities of H. drasticus latex, the objective of the present study was to verify the antibiofilm activity of its latex. The latex was extracted from H. drasticus specimens, where a small area of the vegetal bark (5x30cm) was removed, later the latex was dissolved in water and lyophilized. For the microbiological assays, the ATCC standards of Pseudomonas aeruginosa and Staphylococcus aureus were cultured in BHI broth for 24 hours at 37 ?C. The assay was performed with flat-bottomed 96 well plates, and the latex extracted from H. drasticus and bacterial suspensions were added to the BHI broth. Negative Control (NC) was used as broth BHI and Positive Control (PC), the PA01 strain of P. aeruginosa as this strain is indicated as a positive control for biofilm assays. The plates were then incubated at 37 ?C for 24 hours. After this period, the absorbance was read in an ELISA reader with wavelength of 570 nm, then the samples were classified. The antibiofilm activity of the latex extracted from H. drasticus was classified as non-adherent on the species P. aeruginosaand S. aureus. Thus, the study revealed that H. drasticus latex has a potential antibiofilmaction, however, additional studies should be carried out in order to evaluate other aspects associated with the biological activities performed by this plant species.

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[Danielle Feijo de Moura, Carlos Roberto Weber Sobrinho, Dayane de Melo Barros, Jose Luciano Brainer de Farias Filho, Alexandre Gomes da Silva, Tamiris Alves Rocha, Sheila Taise Fernandes e Silva, Marllyn Marques da Silva, Rene Duarte Martins, Marcia Vanusa da Silva. (2019); EVALUATION OF ANTIBIOFILM ACTIVITY OF THE LATEX EXTRACTED FROM HIMATANTHUS DRASTICUS (MART.) PLUMEL (JANAGUBA). Int. J. of Adv. Res. 7 (Jan). 446-450] (ISSN 2320-5407). www.journalijar.com

Danielle Feijo de Moura
Mestre em Saude Humana e Meio Ambiente – Centro Academico de Vitória, Universidade Federal de Pernambuco, CAV/UFPE – Pernambuco.

DOI:

Article DOI: 10.21474/IJAR01/8348      
DOI URL: http://dx.doi.org/10.21474/IJAR01/8348

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