04Jan 2018

EVALUATION OF TOXIC EFFECTS OF CHEMOTHERAPEUTIC DRUGS ON NORMAL JAW BONES USING OSTEOPONTIN: AN EXPERIMENTAL STUDY.

  • Assistant Lecturer of Oral Pathology, Faculty of Dentistry, October 6 University, Egypt.
  • Lecturer of Oral Pathology, Faculty of Dentistry, October 6 University, Egypt.
  • Assistant Lecturer of Oral Pathology, Faculty of Dentistry, October 6 University, Egypt.
  • Lecturer of Oral Pathology, Faculty of Dentistry, BaniSuef University, Egypt.
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Background: Chemotherapy does not differentiate between rapidly dividing cancer cells and normal cells that divide at higher rates as bone forming cells. Methotrexate and 5-fluorouracil are chemotherapeutic drugs with high potentiality to damage bone forming cells by blocking their DNA synthesis which leads to their suppression and in turn impaired bone formation mainly by dysregulation of Runx2 and osterix associated with osteoblastic cell differentiation. Aim: Compare the toxic effects of methotrexate and 5-flurouracil on normal bone cells. Material and methods: A total of 30 rats were divided into three groups. Group I was control group, Group II was given a single 20 mg/kg intraperitoneal dose of MTX and Group III was given a single 150 mg/kg intraperitoneal dose of 5-FU. Four days later, the rats were euthanized and their mandibles were dissected, immediately fixed in 10% formalin, processed and prepared for histopathological and immunohistochemical examinations using osteopontin. Statistical analysis was performed using ANOVA test where P values < 0.05 were statistically significant. Results: Variable degenerative effects on the bone forming cells were observed where such effects were higher in 5-FU group compared to the MTX group. Positive osteopontin expression and higher number of bone formative cells were detected in the control group followed by MTX group then the 5-FU group. Low osteopontin expression was correlated with decreased number of bone cells and subsequent decreased jaw bone density and formation. Conclusion: 5-FU is more cytotoxic to the normal bone forming cells than MTX.

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[Amina Fouad Farag, Nadia Fathy Hassabou, Yasmine Alaa El-Din and Ahmed Nabil Fahmi. (2018); EVALUATION OF TOXIC EFFECTS OF CHEMOTHERAPEUTIC DRUGS ON NORMAL JAW BONES USING OSTEOPONTIN: AN EXPERIMENTAL STUDY. Int. J. of Adv. Res. 6 (Jan). 430-436] (ISSN 2320-5407). www.journalijar.com

Amina Fouad Farag
Assistant Lecturer, Oral Pathology Department, Faculty of Dentistry, October 6 University, Egypt.

DOI:

Article DOI: 10.21474/IJAR01/6237      
DOI URL: http://dx.doi.org/10.21474/IJAR01/6237

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