20May 2017

PREPARATION AND CHARACTERIZATION OF SHRIMP DERIVED CHITOSAN AND EVALUATION OF ITS EFFICIENCY AS BEE VENOM DELIVERY FOR CANCER TREATMENT.

  • Faculty of Science, Al-Azhar University (Girls branch).
  • The Holding Company for Production of Vaccines, Sera and Drugs (VACSERA).
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Chitosan is an amino polysaccharide prepared by processing shrimp skeleton wastes which involves partial deacetylation of chitin. Many biochemists have found that chitosan as biocompatible, biodegradable and non toxic compound which made wide applicability in conventional pharmaceutics as a potential formulation excipient. The nanoparticles were loaded with venom of Apismelliferabees and cytotoxicity against colon cancer (CaCO2) was evaluated. The crude chitin was collected from exoskeleton of Penaeuskerathurus specimens and was processed to obtain chitosan(CS). Chitosan nanoparticles (CS NPs) were prepared by the ionotropic gelation method of chitosan cations with sodium tripolyphosphate (TPP) anions.The encapsulation efficiency and loading capacity of the bee venom on the prepared chitosan nanoparticles were evaluated. The cytotoxicity of bee venom (BV) alone,chitosan nanoparticles andbee venom loaded chitosan nanoparticles againstCaCo2 cells was conducted using MTT staining assay.Data recorded revealed thatthe combination of bee venom (BV) with chitosan nanoparticles enhanced the inhibitory effect on colon cancer cells more than treatment with BV alone and chitosan nanoparticles without venom.

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[Fatma. A. Taher, Walaa. A. Moselhy, Aly. F. Mohamed, Samia. E. El-Didamony, Karima. M. Metwalley and Zayed. A.B. (2017); PREPARATION AND CHARACTERIZATION OF SHRIMP DERIVED CHITOSAN AND EVALUATION OF ITS EFFICIENCY AS BEE VENOM DELIVERY FOR CANCER TREATMENT. Int. J. of Adv. Res. 5 (May). 370-388] (ISSN 2320-5407). www.journalijar.com

Aly, F. Mohamed
The Holding Company for Production of Vaccines, Sera and Drugs

DOI:

Article DOI: 10.21474/IJAR01/4122      
DOI URL: http://dx.doi.org/10.21474/IJAR01/4122

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