25Mar 2017

DIFFERENTIAL ACTIVITY OF FOUR SELECTED ENZYMES IN THE PISTILS AND POLLEN GRAINS OF TWO VARIETIES OF HAMELIA PATENS JACQ. (RUBIACEAE) FOLLOWING COMPATIBLE AND INCOMPATIBLE POLLINATION.

  • Department of Botany, University of Kerala, Kariyavattom, Thiruvananthapuram-695581.
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Hamelia patens Jacq.commonly called fire bush, is a fast growing, semi woody and evergreen perennial shrub of the family Rubiaceae. It is a native of central and southern Florida. It is chiefly grown for the showy bunch of beautiful flowers. In tropical America, local people make use of the extract of its leaves and stem, for curing diseases such as skin rashes, sores, insect sting and various fungal diseases of the skin because of its antibacterial and antifungal properties. In India this plant is particularly cultivated on a wider scale in home gardens as an ornamental plant. In H. patens, sexual reproduction is difficult due to self- incompatibility, while crossing between morphologically different plants of two accessions gives viable seeds. In the present study two varieties of H. patens, H. patens variety patens and H. patens variety glabrawas chosen to study the activity of four enzymes, namely, peroxidase, acid phosphatase, polyphenol oxidase, esterase and total protein. Three developmental stages were identified for both the plants. Controlled pollinationswere carried out in the two plants. Stigma of two varieties were pollinated with its own pollen and also pollen from other variety. Crossed stigma showed higher activity of all the enzymes than self- pollinated and stigma from flowers before anthesis. The aim of the study was to understand if pollination with self and crossed pollen grains has any influence on the activity ofthe four selected enzymes in post pollinated pistils. The enhanced activity of these enzymes indicates their role mainly in the defence mechanism than anything to do with stigma receptivity. The results have been discussed in the light ofthe available literature.

  1. M N., Sathya, C., &Prabakaran, R. (2013).Invitro Antifungal potentials of Hamelia patens Jacq (Rubiaceae) Aqueous extracts of leaves, Flower and fruits. Biosciences Biotechnology Research Asia, vol 10(2), 699-704.
  2. Ahmad, A., Pandurangan, A., Singh, N., &Ananad, P.(2012). A minireview on chemistryand biology of Hamelia patens (Rubiaceae).Pharmacog J, 4, 1–4.
  3. Aquino, R., Ciavatta, M.L., De Simone, F., &Pizza, C.(1990). A flavone glycoside fromHameliapatens.Phytochemistry, 29, 2359–2360.
  4. Bashan, Y., Okon, Y., &Henis, Y. (1987). Peroxidase,Polyphenol Oxidase and Phenol in Relation to Resistance AgainstPseudomonasSyrigaepvTomatoin Tomato Plants. J.Bot, 65, 366-372.
  5. Bradford, M.M. (1976).A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding.Biochemistry, 72, 248-254.
  6. Bredemeijer, G M M. (1982). Mechanism of Peroxidase Isoenzyme Induction in Pollinated NicotianaAlataTheor. Appl. Genet. 62, 305-309.
  7. Bredemeijer, G M M. (1984). The Role of Pereoxidasesin Pistil-pollen Interactions. Appl. Genet. 68, 193-206
  8. Bredemeyer, G MM. (1975).The effect of peroxidase on pollen germination and pollen tube growth in vitro. Cell Incompatibility Newslett,5, 34–39.
  9. Bredemeyer, G MM. (1979).The distribution of peroxidase isozymes, chlorogenic acid oxidase and glucose-6-phosphate dehydrogenase in transmitting tissue and cortex of NicotianaalataActa Bot. Neerl, 28,198–203.
  10. Bredemeyer, G MM., &Blaas, J A. (1975).Possible role of a stylar peroxidase gradient in the rejection of incompatible growing pollen tubes.Acta Bot. Neerl, 24, 37–48.
  11. Brien, M O., Kapfer, C., Major, G.,Laurin, M., Bertrand, C., Kondo, K., Kowyama, Y. &Matton, D.P. (2002). Molecularanalysis of the stylar expressed Solanumchacoensesmall asparagine-rich protein family related to theHT modifier of gametophytic self-incompatibility in Nicotiana. J, 32, 985–996.
  12. Camporese, A., Balick, M J., Arvigo, R., Esposito, R G., Morsellino, N., De Simone, F.,&Tubaro, A. (2003). Screening of anti-bacterial activity of medicinal plants fromBelize (Central America). J. Ethnopharmacol, 87, 103–107.
  13. Castro, A J A., Ramos, S B., Morales, AH., Morales, J R Z., Chavez, M M G.,& Alvarez, C C. (2015). RevistaBrasileira de Farmacognosia, 25, 170-176.
  14. Cavanagh, F. (1963). Analytical Microbiology, Academic Press Inc, New York,
  15. Cheong, Y H., Chang, H S., Gupta, R., Wang, X., Zhu, T., & Luan, S. (2002). Transcriptional Profiling Reveals Novel Interactions between Wounding, Pathogen, Abiotic Stress, and Hormonal Responses in Arabidopsis.PlantPhysiol, 129,661-677.
  16. Ching, T M., Lin, T., & Metzger, R J. (1987). Purification and Properties of Acid Phosphatase from Plump and ShriveledSeeds of Triticale.PlantPhysiol, 84, 789-795.
  17. Dafni, A. &MotteMaues, M. (1998). A Rapid and Simple Procedure to Determine Stigma Receptivity. Plant Reprod.11, 177-180.
  18. Delannoy, E., Jalloul, A., Assigbetse, K., Marmey, P., Geiger, J.P., Lherminier, J., Daniel, J.F., Martinez, C. & Nicole, M. (2003). Activity of Class III Peroxidases in the Defense ofCotton to Bacterial Blight. Plant Microbe Interactions,16, 1030-1038.
  19. Dhaliwal, A S.,& Malik, C P.,(1985). Enzyme changes in the stigma style tissue of Brassica campestris Toriafollowing compatible and incompatible pollinations. In Recent advances in pollen research (T. M. Varghese(ed.)), Allied Publisher Pvt Ltd, New Delhi, 15–23.
  20. Do, H.M., Hong, J.K., Jung, H.W., Kim, S.H., Ham, J.H., &Hwang, B.K. (2003). Expression of Peroxidase-like Genes, H2O2 Production, and Peroxidase Activity during the
  21. Duke, J. (2007). Dr. Duke\'s Phytochemical and Ethnobotanical Databases,19(2007).
  22. Dupuis, I., & Dumas, C. (1990). Biochemical Markers of Female Receptivity in Maize (Zea mays ) Assessed Using inVitroFertilization.Plant Sci. 70, 11-20.
  23. Galen, C.,& Plowright, R.C. (1987). Testing Accuracy of using Peroxidase Activity to Indicate Stigma Receptivity. J. Bot,65, 107-111.
  24. Heslop-Harrison, J. (1975). Incompatibility and Pollen-stigma Interaction. Rev. Plant. Physiol,26, 403-425.
  25. Heslop-Harrison, Y., &Shivanna, K R. (1977). The Receptive Surface of the Angiosperm Stigma. Bot, 41, 1233-1258.
  26. Hiscock, S J., Bown, D., Gurr, SJ.,& Dickinson, H G. (2002).Serine Esterases are Required for Pollen Tube Penetration of the Stigma in Brassica. Plant Reprod. 15, 65-74, 2002.
  27. Hoopen, R.,Harbord.,Maes,R.M.,Nanninga, N., &Robbins, T P. (1998). The self-incompatibility (S) locus inPetunia hybrid is located on chromosome III in a region, syntenic for the Solanaceae. J, 16, 729–734.
  28. Hypersensitive Response to XanthomonasCampestrisVesicatoriainCapsicum Annuum.Molec.Plant MicrobeInteractions.16, 196-205.
  29. Ibrahim, M B.(1997). Antimicrobial effects of extract leaf, stem and root bark of Anogeissusleiocarpus on Staphylococusaureaus, Streptococcus pyrogenes, Escherichia coli and Proteus vulgaris. J. Pharma,Devpt. 2, 20-30.
  30. Irobi, O N., Young, M. & Anderson, W.A. (1996). Antimicrobial activity of Annatto (Bixaorellana) extract. International Journal of Pharmacy, 4, 87-90.
  31. Jorgensen, E., &Vejleby, K A.,(1953). New polyphenol oxidase test. Plant, 6, 533-537.
  32. Kohn, J R., &Waser, N M. (1985). The Effect ofDelphiniumNelsoniion Seed Set in IpomopsisAggregata, a Competitor for Hummingbird Pollination. J. Bot,72,1144-1148.
  33. Lachoria, L., Jain, P.C. & Agarwal, S.C. (1999). Activity of some plant extracts against dermatophytes. Hindustan Antibiot. Bull, 41, 17-21.
  34. Lavithis, M., &Bhalla, P L., (1995). Esterases in Pollen and Stigma of Brassica. Plant Reprod. 8, 289-298.
  35. Linskens, H F., Havez,R.,Linder, R.,Salden,M., Randoux,A., Laniez,D., &Coustant,D. (1969).Etude des glycanne-hydrolases au cours de la croissance du pollen chez Petunia hybrid auto incompatibillity.R. Acad.Sci. (Paris), 1857–1885.
  36. Liogier, H A, (1999). Plantasmedicinales de Puerto Rico y del Caribe.Iberoamericana de Ediciones, Inc. San Juan, PR., 566.
  37. Louis, CF., Raj, RSN.,&Radhamany,PM.(2012).Identification and characterisation of incompatibility factors in Hamelia patens(Rubiaceae).J HorticSci&Biotech, 87,531-538.
  38. Louis, CF.,&Radhamany, P.M.(2009). Pollen-pistil interactions in Hamelia patens (Rubiaceae). J Indian Bot Soc,88, 92-97.
  39. Lowry, O H., Roserbrough, N J., Farr, A L.,& Randall, R (1951). Protein measurement with folin-phenol reagent.J. Biol. Chem., 193, 256–275.
  40. Malik,C. P.,& Singh, M B. (1980). Plant enzymology and histoenzymology, Kalyani Publishers., 431.
  41. Mayer, A M., &Harel, E H. (1979). Polyphenol Oxidase in Plants.Phytochemistry,18, 193-215.
  42. McInnis, S M., Emery, D C., Desikan, R P., Hancock, J T., &Hiscock, S J. (2006). “The Role of Stigma Peroxidases in Flowering Plants: Insights from further Characterization of a
  43. Neog, B., &Singh, I D. (2003).Study of floral biology of some cultivated tea (Camellia ) growing in the upper Assam region of the northeast India. J. Pl. Crops, 1, 50-52.
  44. Nettancourt, D. (1977). Incompatibility in angiosperms, Springer-Verlag, 230.
  45. Paniagua-Vega, D., Cerda-García-Rojas, C.M., Ponce-Noyola, T.,& Ramos-Valdivia, A.C. (2012). A new monoterpenoidoxindole alkaloid from Hameliapatensmicropropagated plantlets. Prod. Commun, 7, 1441–1444.
  46. Reyes-Chilpa, R., Rivera, J., Oropeza, M., Mendoza, P., Amekraz, B., Jankowski, C.,&Campos, M., (2004). Methanol extracts of Hamelia patens containing oxindolealkaloids relax KCl-induced contraction in rat myometrium. Biol. Pharm. Bull, 27, 1617–1620.
  47. Salud, P G., Miguel A., Zavala, S., Rosario Vargas, S., Edgar Hernandez, Z., Rosa, M.,& Perez, G. (1996).Antidiarrhocal activity of Hamelia patens Mehanol extract in rats and mice. Phytotherapy Research, 10, 686-688.
  48. Sandhya, S., SaiKumar, P., Vinod, K. R., David Banji., & Kumar, K. (2011). Plants as potent antidiabetic and wound healing agents - A review.Hygeia J. D. Med, 3, 11-19.
  49. Sapana, K., Parul, S., Tribhuwan, S., &Rekha, V. (2012). Anthelmintic and antimicrobial activity of Hamelia patens (Rubiaceae).International Journal of Natural Products Research,1, 54-56.
  50. Sarvesh, A., &Reddy, T P. (1988). Peroxidase, polyphenol oxidase, acid phosphatase and alkaline inorganic pyrophosphataseactivities during leaf senescence in varieties of castor (Ricinuscommunis).Indian J. ExplBiol.26, 133–136.
  51. Sawhney, S., Basra, A S. &Kohli, R K. (1981). Enzyme activity and electrophoretic patternofisoenzymes of peroxidase, esterase and alkaline and acid phosphatase in relation to flowering in Amaranthusviridis - Quantitative SD Plant.Biol. Plant, 23, 335-341.
  52. Shrisha, D L., Raveesha, K A., &Nagabhushan.(2011). Bioprospecting of selected medicinal plants for antibacterial activity against some pathogenic bacteria.Journal of Medicinal Plants Research, 5, 4087-4093.
  53. Siegel, S M., &Galston, A W. (1955). Peroxide genesis in plant tissues and its relation to indole acetic acid distribution. Biochem. Biophys, 54, 102.
  54. Stigma-specific Peroxidase (SSP) from SenecioSqualidus(Asteraceae).” Exp. Bot. 57, 1835-1846.
  55. Stonier, T.,Stasinos, S. and Murthy Reddy K.B.S.(1979): The masking peroxidase-catalysed oxidation of IAA inVigna. Phytochemistry.,18, 25–28.
  56. Stpiczynska, M. (2003). Stigma Receptivity during the Life Span of PlatantheraChloranthaCusterRchb) Flowers.Acta Biol. Cracovia Series Bot. 45, 37-41.
  57. Taylor, P., Arsenak, M., Abad, M. J., Fernandez, A., Milano, B., Gonto, R., Ruiz, M. C., Fraile, S., Taylor, S., Estrada, O., &Michelangeli, F. (2013).Screening of Venezuelan medicinal plant extracts for cytostatic and cytotoxic activity against Tumor cell lines. Phytotherapy Research, 27, 530-539.
  58. Tyagi, M., Kayastha, A M., & Sinha, B. (2000). The Role of Peroxidase and Polyphenol Oxidase Isozymes in Wheat Resistance to Biol. Plant,43, 559-562.

[Renjumol. R and Radhamany P. M. (2017); DIFFERENTIAL ACTIVITY OF FOUR SELECTED ENZYMES IN THE PISTILS AND POLLEN GRAINS OF TWO VARIETIES OF HAMELIA PATENS JACQ. (RUBIACEAE) FOLLOWING COMPATIBLE AND INCOMPATIBLE POLLINATION. Int. J. of Adv. Res. 5 (Mar). 636-644] (ISSN 2320-5407). www.journalijar.com

Renjumol. R
Department of Botany, University of Kerala, Kariyavattom, Thiruvananthapuram-695581

DOI:

Article DOI: 10.21474/IJAR01/3554      
DOI URL: http://dx.doi.org/10.21474/IJAR01/3554

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