15Aug 2017

A STUDY OF ISOLATION AND IDENTIFICATION OF CAMPYLOBACTER SPECIES, LISTERIA MONOCYTOGENES E. COLI O157:H7 AND SALMONELLA SPECIES FROM RAW CHICKEN CARCASSES AND BEEF MEAT BY MULTIPLEX POLYMER CHAIN REACTION IN BAGHDAD CITY.

  • Medical Technical Institution /Al-Mansur.
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The study designed to determine the distribution of a major important food pathogens including Campylobacter spp, Listeria monocytogenes, E. coli O157:H7 and Salmonella spp from raw chicken carcasses and beef meat by using multiplex PCR. A total of 100 raw chicken carcasses and beef meat samples were collected from different markets and butcher's shops in Al-Karkh side of Baghdad city and analyzed for the presence of these types of bacteria and their susceptibilities to some antibiotics was investigated ,the results showed that the prevalence of C.jejuni was (31%) , C. coli (12%), L. monocytogenes (8%), E. coli O157:H7 (42 %) and Salmonella spp (7%) from the total samples .The result of the susceptibility test showed that C.jejuni isolates were susceptible to Ciprofloxacin, Gentamycin, Streptomycin and chloramphenicol, (87,87,84,80)% respectively and both Erythromycin,Neomycin (77%) , C. coli isolates were susceptible to Ciprofloxacin (84%) and (75%) for each Gentamycin, Streptomycin ,chloramphenicol and Neomycin ,while L. monocytogenes isolates were susceptible to the most used antibiotics as following Amikacin, Erythromycin, Oxytetracycline, Nalidixic acid ,Cephalothin, Gentamycin, Ampicillin and Streptomycin (100%). E. coli O157:H7 isolates were susceptible to Nalidixic acid and Gentamycin (98%,96%%) and Salmonella spp isolates were susceptible to Gentamycin ,Cephalothin and (86%,71%) .

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[Zena K. Khalil. (2017); A STUDY OF ISOLATION AND IDENTIFICATION OF CAMPYLOBACTER SPECIES, LISTERIA MONOCYTOGENES E. COLI O157:H7 AND SALMONELLA SPECIES FROM RAW CHICKEN CARCASSES AND BEEF MEAT BY MULTIPLEX POLYMER CHAIN REACTION IN BAGHDAD CITY. Int. J. of Adv. Res. 5 (Aug). 900-907] (ISSN 2320-5407). www.journalijar.com

Zena K.Khalil
Medical Technical Institution /Al-Mansur

DOI:

Article DOI: 10.21474/IJAR01/5134      
DOI URL: http://dx.doi.org/10.21474/IJAR01/5134

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