We developed a Japanese version of the Trypophobia Questionnaire (TQ-J) that measures proneness to disgust for a cluster of objects. Its validity was examined using discomfort ratings for trypophobic, neutral, and unpleasant images, and assessment of trait anxiety. The TQ-J showed a one-factor structure, and sufficient internal consistency and test–retest reliability. Its strong correlations with discomfort ratings for trypophobic images indicated optimal convergent validity. It did not correlate with ratings for neutral images, and only weakly correlated with ratings for unpleasant ones and trait anxiety; these findings indicated sufficient discriminant validity. Our results confirmed the reliability and validity of the TQ-J.