In vitro Properties of Methanol Extract and Sodium Alginate of Sargassum polycystum C. Agardh Brown Seaweed Collected from Malaysia

The edible brown seaweed, Sargassum polycystum C. Agardh was harvested from the coastal region of Malaysia. In this study, analysis of the nutrition and metal content in the methanol extract showed positive for sodium, potassium, magnesium, vitamins A and E and arsenic contamination. The brine shrimp lethality assay (BSLA) revealed the extract to be non-toxic with LC50 value of 15.60 mg mL−1 (LC50 > 1). The antioxidant and antibacterial activities of the methanol extract were measured through various bioassays. The structural and physicochemical characterisation of the NaAlg, analysed through the 1H-NMR analysis revealed the M:G ratio of NaAlg at 0.733 with mannuronic (M) and guluronic (G) fractions at FM = 0.423 and FG = 0.577, respectively. The degraded NaAlg through methods of ultraviolet irradiation and sonication showed an increment in the in vivo antioxidant activities at intervals of 15 min, 30 min, 60 min, 90 min and 120 min. The Fourier transform infrared spectroscopy (FTIR) spectra of polysaccharides taken before and after UV irradiation showed breakage of covalent bonds and thus, increase in the intensity of both O–H and C–O stretching vibrations. Therefore, the increment in antioxidant activities observed in the treated samples were related to changes seen in their molecular structures.


INTRODUCTION
The brown seaweed, Sargassum polycystum is a member of the Phaeophyceae group that dominates the benthic reefs of tropical and temperate region (Hwang et al. 2006). There are over 400 species of Sargassum distributed in the warm and temperate waters of Indo-western Pacific region, where Malaysia accommodates about 21 species of the Sargassum genus (Noiraksar & Ajisaka 2008;Wong & Phang 2004). The intertidal and subtidal Sargassum beds in the marine ecosystem are essential in providing food, habitat and nursery grounds for marine organisms while it remains equally important for people who harvest Sargassum for food, alginates, feed and also bioactive compounds (Redmond et al. 2014).
Traditionally, seaweeds are regarded as a health food in Asian countries and were consumed as a staple item of diet, thus opening opportunities in the development of various seaweed food products, such as noodles, tea, wine and soup (Rupérez 2002). Generally, the mineral content (8% to 40%) and elements found in seaweeds were reported to be higher than that of land plants and animal products (Ito & Hori 1989;Ortega-Calvo et al. 1993). Nevertheless, the nutritional contents of seaweed are dependent on the type of seaweed, mineralization methods, environmental factors and the origin of seaweeds (Rupérez 2002). The complex compounds identified in the seaweeds with varying structures and activities from terrestrial plants could be the results of their response to ecological pressure and effective defence mechanism in order to adapt to the harsh environmental conditions in the marine ecosystem (Pérez et al. 2016). Approximately 80 of the 400 known Sargassum species were screened and analysed for their bioactive metabolites. Pharmacological properties and novel compounds revealed that the major contributors for the therapeutic effects of Sargassum is derived from phlorotannins, fucoidans and monoterpenoids. Sodium alginate is generally the main cell wall components of seaweeds with structural functions. Polyunsaturated fatty acids, carotenoids, and other phenolic are among other bioactive compounds that has been identified in brown seaweeds (Pérez et al. 2016).
In view to the current demand of seaweeds and the few studies on edible S. polycystum from Malaysia, this paper provides an overview of the biochemical, mineral contents and bioactivities of the methanol and sodium alginate extracts. Thus, the information obtained from this current study would add economic value to marine seaweeds of this part of the world.

Sample Collection and Extraction
The S. polycystum sample was collected from Teluk Kemang, Port Dickson, Malaysia and rinsed with 0.1% sodium chloride. The sample was pulverized and macerated in methanol (1:10; w.v −1 ) (EMSURE, USA) followed by agitation at 200 rpm in room temperature for 12 h to 16 h. The solvent was evaporated to dryness using a vacuum rotary evaporator.

Phytochemical Analysis
The presence of terpenoids, cardiac glycosides, flavonoids, saponins, alkaloids and tannins in the crude methanol extract were analysed qualitatively based on the methods of Harbone (1973) and Trease and Evans (1983).

Antioxidant Assays
The DPPH, FRAP and ABTS assays were performed according to methods described by Rajauria et al. (2010), Benzie and Strain (1996) and Boonchum et al. (2011), respectively. Briefly, 600 µL of 0.16 mM DPPH • solution was added to 400 µL of extract at different concentrations (10.00 to0.31 mg mL −1 ) and incubated in the dark at 37°C for 30 min and the absorbance of the mixture was read at 540 nm.
The ABTS assay was performed by preparing stock solutions of 14 mM ABTS •+ and 4.9 mM potassium persulfate. The working solution was prepared fresh at every run and it consists of equal volumes of the respective stock solution. The mixture was allowed to react in the dark for 12 h to 16 h at room temperature and the absorbance was adjusted to 0.70 ± 0.02 nm at 734 nm. In the cuvette, 700 µL extracts were allowed to react with 300 µL of the ABTS •+ solution for 10 min in the dark at 37°C and absorbance was measured at OD 734 nm.
The extract concentration value was plotted vs. % of inhibition of free radicals and the EC 50 value was obtained by linear regression. The capacity of the extract to scavenge the DPPH and ABTS radicals were calculated using the following equation: The ability of the sample to reduce ferric ion (Fe 3+ ) to ferrous ion (Fe 2+ ) was measured through the FRAP assay. The stock solutions included, 300 mM acetate buffer, pH 3.6; 10 mM TPTZ (2, 4, 6-tripyridyl-s-triazine) in 40 mM HCl and 20 mM FeCl 3 ·6H 2 O. The fresh working solution prepared by mixing the solution in the ratio 10:1:1 (v:v:v) of stock chemicals and preheated at 37°C before use. Briefly, 600 µL of the FRAP reagent was mixed with 400 µL of the samples (10.00 to 0.31 mg mL −1 ) and incubated in the dark for 30 min at room temperature. Equal volumes of sample and FRAP reagent was allowed to react in the dark and measured at OD 593 nm. The ability of the extract to reduce Fe 3+ to Fe 2+ was expressed as mM Fe(II) g 1 using a calibration curve of FeSO 4 (0.1 to1.0 mM).

Total Phenolic Content (TPC)
The TPC was analysed according to Devi et al. (2008) with some modification using Folin-Ciocalteu's method with Gallic acid as a standard. An aliquot of 100 µL samples (10.00 to 0.31 mg mL −1 ) was mixed with 400 µL of 2% Na 2 CO 3 and incubated for 2 min at room temperature. Five hundred microliter of 50% Folin-Ciocalteau's phenol reagent was added into the mixture and allowed to stand in the dark for 30 min at room temperature. After incubation, the absorbance was measured at 720 nm. The TPC was expressed as mg Gallic acid equivalent, GAE g −1 extract.

Thin Layer Chromatography
The profile of pigments present in the methanol extract was accessed with the silica-TLC plate following the methods reported by Sathya et al. (2017) with modifications. The pre-coated TLC plates (20 cm × 20 cm); 0.25 mm thickness silica gel 60 F 254 aluminium plates (Merck) were spotted with the crude extract and subjected to the following mobile phase: hexane:ethyl acetate (3:1; v.v −1 ). The blue spot visualised by spraying the plates with a mixture of 1% potassium ferricyanide and 1% ferric chloride and yellow spot with exposure to DPPH • reagent is indicative of their antioxidant properties.
Based on the methods by Rajauria and Abu-Ghannam (2013), the antioxidant activities of fractions separated on the TLC can be quantified with the antioxidant assays. Firstly, the coloured bands on TLC were scrapped and dissolved separately in methanol and centrifuged at 10,000 rpm for 15 min. Subsequently, the supernatant was collected and evaporated to dryness before subjecting to FRAP and DPPH antioxidant assays.

Analysis of Ash Content
The analysis of the ash content follows the method described by Pearson (1976). The air-dried crude sample was placed in a silica crucible and kept in a muffle furnace and ignited gradually by increasing the temperature up to 500°C to 600°C. The ash was cooled in a desiccator for 30 min and weighed. The total ash content was expressed as g 100 mL −1 of the sample.

Determination of Minerals and Metal Elements
The presence of minerals (calcium, sodium, magnesium and potassium) and metal (arsenic, lead, mercury and cadmium) elements in the dry ash samples were determined according to the modified method as described in the U.S. Environmental Protection Agency (EPA) Revision 2, 1996 andPharmacopoeia (2013), respectively. The concentration of the minerals was determined using inductively coupled plasma mass spectrometry (ICP-MS) and metal elements with inductively coupled plasma optical emission spectroscopy (ICP-OES).

Fat-soluble Vitamins
The XDB-C18 column was used (5 μm, 4.6 × 150 mm), the solvent was 90% acetonitrile and 10% methanol, and UV detection was recorded at 324 nm for vitamin A and the fluorescence for vitamin E was detected at Ex λ = 325 and Em λ = 480. Both vitamin A (retinyl palmitate) and E (α-tocopherol) were identified in the extract by comparing their retention times with those of authentic standards. The concentrations of the fat-soluble vitamins were calculated from the integrated areas of the sample and the corresponding standards.

Antibacterial Assays
The bacteria strains were purchased from the American Type Culture Collection (ATCC) USA; Escherichia coli (ATCC 25922), E. coli (ATCC 35218), Bacillus subtilis (ATCC 6633), Bacillus cereus (ATCC 11778), Staphylococcus aureus (ATCC 6538), S. aureus (ATCC 6854), Listeria monocytogenes (ATCC 7646) and Pseudomonas aeruginosa (ATCC 27853). The S. aureus, B. subtilis, and E. coli were cultured on Muller-Hinton Agar (MHA) and P. aeruginosa and L. monocytogenes on nutrient agar (NA) under aerobic condition for 12 h to18 h at 37°C. The minimal inhibitory concentration (MIC) values were determined using a standard susceptible agar dilution method according to the Clinical Laboratory Standards Institute (CLSI 2006) guidelines. The density of the inocula required for the assay was adjusted to 0.5 McFarland standard (1 × 10 7 colony-forming units mL −1 ). The sample was dissolved and diluted with DMSO to obtain a range of concentrations (10.00 to 0.31 mg mL −1 ). Then, 50 µL of bacterial suspension was transferred to each well containing 50 µL of extracts and incubated at 37°C for 12 h to18 h. The MIC was defined as the lowest concentration of the compounds to inhibit the growth of microorganisms detected following the addition of 20 µL of 0.20 µg mL −1 iodonitrotetrazolium chloride (INT) indicator.
The lowest concentration of the extract that showed no visible growth after subculture was described as minimum bactericidal concentration (MBC).
MBC's was determined by transferring 10 µL of each medium with no visually detectable bacterial growth in fresh media. The negative control used was DMSO, while the positive control was ampicillin and tetracycline.

Brine Shrimp Lethality Assay (BSLA)
The dry cysts (Artemia nauplii) were hatched in artificial sea water (3.8%) in a container attached to an air pump with regular air flow. The hatching chamber was constantly illuminated and incubated for 36 h at room temperature (22°C to 29°C) to obtain the active nauplii. Then, 10 to15 nauplii were transferred into the 16-well plates, separately containing methanol extracts at different concentrations (10.00 to 0.63 mg mL −1 ). After 24 h of incubation, the numbers of living nauplii were examined and counted. The lethal concentration required to kill 50% of the nauplii (LC 50 ) with 95% confidence intervals was calculated by Finney's table. The median lethal concentration (LC 50 ) for the test samples has been obtained by a plot of the percentage of the shrimps killed against the logarithm of the sample concentration and the toxicity classification was based on the study by Carballo et al. (2002).

Extraction of Sodium Alginate
The NaAlg was extracted from S. polycystum following the method by Fertah et al. (2017) with minor modifications. The powdered seaweed was immersed in 0.2M hydrochloric acid at room temperature for 24 h. Subsequently, the residues were rinsed with distilled water and agitation for 5 h with 2% sodium carbonate. The extracts were filtered and later precipitated with ethanol. The yield of the crude NaAlg samples was dried overnight at 60°C and expressed as a percentage (%) of dry weight (DW).

Degradation of NaAlg
To prepare the degraded NaAlg, the polymer samples were subjected to UVirradiation and sonication. The ultraviolet-C (UV-C) irradiation was performed in the UV cabinet at 254 nm monochromatic output. The 1% NaAlg solution was placed in a petri dish and exposed to the UV lamp at time intervals of 15 min, 30 min, 60 min, 90 min and 120 min with constant mixing at every 15 min intervals.
The polysaccharide was subjected to ultrasonic waves (Elma Elmasonic S30H) at 28 kHz frequency, 1 cm above the transducer. Ten millilitres NaAlg samples were treated for 15 min, 30 min, 60 min, 90 min and 120 min at 37°C water bath with stirring at regular intervals.

Chemical Characterisation
Infrared spectra of the polysaccharide samples analysed with a Fourier transform infrared spectroscopy (FTIR) spectrophotometer (Perkin-Elmer Spectrum TM 1000) equipped with attenuated total reflection accessory (ATR) containing a diamond/ZeSe crystal. The NaAlg was dried in the oven at 60°C before the analysis. The ATR-FTIR spectra were recorded in the 650 to 4000 cm −1 range using 16 scans and resolution of 4 cm −1 .
The NaAlg samples were subjected to partial hydrolysis with acid reflux following the methods described by Jensen et al. (2015). The NaAlg solution (0.1%) was prepared with Milli-Q water. The pH of the solution was adjusted to pH 5.6 using HCl (1 and 0.1M) and reflux at 100°C for 1 h. After cooling at room temperature, the pH was adjusted to pH 3.8 and reflux for an additional 30 min. Then, the sample was cooled in ice to stop the hydrolysis, neutralised to pH 7 using NaOH (1 and 0.1UV-CM) and freeze-dried. The 1 H NMR spectra were acquired on 0.1% w/v solutions of NaAlg in D 2 O with a Fourier-transform Bruker 250 BioSpin supplied with an inverse multinuclear gradient probehead with z-shielded gradient coils, and with a silicon graphics workstation, at different temperatures.
Following Grasdalen et al. (1979) method, the composition and the block structure of alginate can be quantitatively derived from the following relationships: The mole fraction of M (F M ) was derived from the normalisation condition: The relations between the mole fractions and the doublet frequencies are given by:

Statistical Analysis
Statistical analyses performed by IBM ® SPSS 21.0 statistical package (Chicago, IL, USA). The data were tested for normality (Shapiro-Wilk's test) and subjected to the test of homogeneity (Levene's test). The data presented as mean ± standard deviation (SD) and the differences compared by analysis of variance (one-way ANOVA). A posthoc test (Turkey's test) analysis performed for any significant differences found between the groups (p-value < 0.05).

Yield of Extract and Phytochemical Analysis
The yield of the methanol extract was 0.57% of DW with the extract tested positive for the presence of tannins, flavonoids, terpenoids and cardiac glycosides.

Antibacterial Activities
The antibacterial activities of the crude methanol extract of the S. polycystum was determined against ten bacterial strains which is reported in Table 1. The extract was most effective against Gram positive bacteria; S. aureus, B. cereus and B. substilis with MIC in the range of 0.31 ± 0.07 to 10.00 ± 0.00 mg mL −1 and MBC in the range of 0.63 ± 0.15 to 10.00 ± 0.00 mg mL −1 . The extract effectively inhibited E. coli and P. aeruginosa, however, showed no bactericidal activities towards those Gram negative strains.

Free Radical Scavenging Ability and TPC
Based on the results obtained from Table 1, the S. polycystum methanol extract was effective in scavenging the DPPH and ABTS radicals with EC 50 values of 2.753 and 0.061 mg mL −1 , respectively. The ABTS radical scavenging activities (55.56% to 88.01%) were higher compared to the DPPH (23.49% to 77.57%). In the FRAP assay, the reducing capacity of the extract was evaluated. Antioxidant activity was expressed as equivalents of mM Fe (II) g −1 extract calculated through the Fe 2 SO 4 linear regression curve with the FRAP value reported for 10 mg mL −1 extract at 1.527 ± 0.057 mM Fe (II) g −1 . Based on the Folin Ciocalteau method, the TPC in the methanol extract (10.00 to 0.31 mg mL −1 ) was calculated in the range of 0.269 ± 0.032 to 85.026 ± 1.078 as mg GAE g −1 .

Pigments and Carotenoids
Methanol extract was subjected to TLC fractionation in triplicates (n = 3) through the silica chromatography plate. The retention factor (R f ) for the corresponding fractions was detected and shown in Table 2. The chromatographic profile of the crude extract resulted in 12 distinctive bands. The TLC profiling showed a strong yellow-orange band (F2: R f = 0.056) that corresponded to the fucoxanthin (standard). Among other pigments reported in brown seaweed were chlorophyll a and chlorophyll c, β-carotene and other xanthophylls. Thus, the orange, yellow, and green bands spotted in the TLC would correspond to some of these pigments. The TLC showed a yellowish-green band  Moreover, the antioxidant activity of the separated compounds on the TLC plate was accessed qualitatively by treatment with FeCl 3 -FeCN 3 and DPPH solution (Table 2). Treatment with FeCl 3 -FeCN 3 showed one distinctive blue spot at F2 (R f = 0.056) which indicated that fucoxanthin has the highest ferric reducing ability. Moreover, F2 showed the highest FRAP value at 53.210 ± 6.870 mg Fe(II) g −1 extract. The analysis of the remaining bands (F3 to F12) exhibited lower ferric reducing ability at values ranging from 32.352 ± 2.087 to 45.330 ± 0.963 mg Fe(II) g −1 extract. In the analysis of TLC with DPPH assay, all the bands appeared yellow after treatment. When quantified, the fractions showed radical scavenging activity with values in the range of 2.889 ± 1.192 to 7.695 ± 0.399 mg AAE g −1 extract.

Toxicity Assay
After 24 h of exposure, the results of the BSLA with varying concentrations of S. polycystum methanol extracts were described in Table 1. The toxicity of the extract was highest at 10 mg mL −1 with 90% mortality of nauplii and 0.63 mg mL −1 was the lowest concentration with no mortality. The LC 50 value of the methanol extract was calculated at 15.60 mg mL −1 and was therefore considered not toxic on the basis that the LC 50 > 1 mg mL −1 . Table 3 shows the total ash alongside some minerals and metal elements in S. polycystum methanol extract. Ash content was reported at 0.06 mg L −1 and among the minerals tested, Na and K were in highest concentration at 9.87 and 5.52 mg 100 mL −1 , respectively. Even though the heavy metal contaminant As (arsenic) was present in the extract at 0.17 mg L −1 , nevertheless, metals such as Pb, Hg and Cd were below the detectable limits ( < 0.01 mg L −1 ). The identification and quantification of vitamins were performed by reversed-phase HPLC. The peak of vitamin A and vitamin E were identified by comparing their retention time with the standards; retinyl palmitate (3.16 min) and tocopherol (13.03 min). The concentration of the vitamins were calculated as 1.21 mg L −1 and 1.79 mg L −1 , respectively. Sodium, Na (mg 100 mL −1 ) 9.87

Yield and Phytochemicals in NaAlg
Ten grams of brown seaweed, S. polycystum, yielded 22.51% NaAlg. The phytochemicals detected in the crude NaAlg were saponins and flavonoids. Tannins, terpenoids, cardiac glycosides and phenolics were absent in the extract.

FTIR and NMR Profile of NaAlg
The spectrum for NaAlg extracted from S. polycystum was in agreement with the finding by Fertah et al. (2017) and Fenoradosoa et al. (2010). The ATR-FTIR spectrum of NaAlg extracted from S. polycystum with functional groups that corresponded to the absorption bands in 4000 to 650 cm −1 range is depicted in Fig. 1. The broad peak that appeared at 3374.12 cm −1 in the range of 3,000 to 3600 cm −1 was assigned to stretching  The 1 H NMR spectroscopy is suitable for characterising both the composition and the distribution sequence of the two uronate residues in alginate samples (Yuan & Macquarrie 2015). In Fig. 2, the 1 H NMR spectra of NaAlg sample showed specific peaks of 5.16 , 4.56, and 4.39 ppm assigned to H1-G (peak A), H1-M + H5-GM (peak B), and H5-GG (peak C), respectively. The

Antioxidant Activities of Degraded NaAlg
The antioxidant activities of the treated polysaccharides via UV-irradiation and sonication were reported in Table 4. The S. polycystum NaAlg showed higher antioxidant activities compared to the commercial samples and showed an increment in activities after treatment for 15 min to 120 min. Overall, the sonicated samples showed an increase in ABTS radical scavenging activity at (0.73% to 11.71%) compared to the UV irradiated sample (2.43% to 9.19%) with increasing exposure time. Nevertheless, the UV irradiated NaAlg showed a higher increment at 15 mins compared to the sonicated sample. The sonicated samples also showed an increment in reducing ability by 28.31% to 61.72% compared to the UV irradiated samples at 39.12% to 50.92%. Comparatively, DPPH • scavenging activity of UV irradiated S. polycystum showed a better increment in activity compared to sonicated samples with 16.37% to 60.99% and 22.19% to 49.16%, respectively.

FTIR Profile
The FTIR was used to access the changes in the functional groups of the treated NaAlg samples by sonication and UV-irradiation before and after treatment. In Fig. 3, the UV irradiated NaAlg spectrum for S. polycystum showed a noticeable increase in intensity at band 3436 cm −1 and 1603 cm −1 , assigned to the stretching vibrations of hydroxyl and carboxylate groups, respectively. However, the band at 1032 cm −1 ascribed to C-O-C groups that showed a decrease in intensity over treatment time. The spectrum profile of the sonicated samples showed no obvious changes at an increasing degradation time, and thus, may indicate that structural changes could have been induced through a different mechanism (Fig. not shown).

DISCUSSION
In the present study, the S. polycystum was isolated from the coastal region, Port Dickson, Malaysia and the crude methanol and sodium alginate extracts were studied on its nutritional content and bioactivities. Among the eight strains investigated, the antibacterial profile of the methanol extract revealed that the Gram positive strains were more susceptible than the Gram-negative strains, E. coli (ATCC 25922; ATCC 35218) and P. aeruginosa (ATCC 27853) that exhibited MIC but no MBC activities. A similar study by Kausalya and Rao (2015) reported that 100 mg mL −1 of the S. polycystum methanol extract was effective in inhibiting several Gram negative strains among which is E. coli.
Even though the extract was not effective against the Gram positive, L. monocytogenes, nevertheless they showed inhibitory and bactericidal activities against the other six strains (Table 1). A review by Jaswir (2014) on the antibacterial activity of several Sargassum species from Malaysia revealed that the methanol extracts of S. binderi and S. plagyophillum inhibited both B. subtilis and S. aureus whereas S. flavellum was only effective against B. subtilis. However, the methanol extract of the Sargassum species studied showed no inhibition against P. aeruginosa and E. coli. Therefore, given the effectiveness of S. polycystum extract against the selected strain among the other Sargassum genus thus, further steps of elucidating the potential compounds and their biochemical pathway are worth exploring. The comprehensive analysis of the antioxidant activities of 10 mg mL −1 of the extract showed high DPPH and ABTS radical scavenging activity at 77.57% and 88.01% and EC 50 values of 2.753 and 0.061 mg mL −1 , respectively ( Table 1). As has been reported in several studies, the antioxidant activities in the Sargassum genus has been well documented. Among the methanol extract of the brown seaweed studied by Matanjun et al. (2009), S. polycystum (FRAP= 366.69 μM mg −1 dry extract; TPC = 45.16 mg PGE g −1 dry extract) exhibited the highest activities compared to Dictyota dichotoma and Padina sp. extracts. The Pearson's correlation analysis showed that there was a positive correlation between TPC with reducing power (p < 0.05; R = 0.940), and DPPH radical scavenging activity (R = 0.859). However, no significant correlation between TPC and ABTS (p > 0.05; R = 0.757) were observed. The strong correlation between FRAP and TPC suggests that polyphenols such as phlorotannins have the ability to reduce (Fe 3+ -TPTZ) to (Fe 2+ -TPTZ) and thus, have the ability to donate electrons to reduce lipid peroxidation, so that they can act as primary and secondary antioxidants (Matanjun et al. 2009;Devi et al. 2011). In addition to that, the qualitative TLC analysis revealed F2 spot (fucoxanthin) as the most apparent compound when treated with FeCl 3 -FeCN 3 and the FRAP value quantified was 53.210 ± 6.870 mg Fe(II) g −1 extract. Therefore, the results suggest that polyphenolic compounds and fucoxanthin may have contributed to the antioxidant activity observed in FRAP assay.
The mineral content of the crude methanol extract of S. polycystum is shown in Table 3. Generally, a lower ash content was measured at 0.06 mg L −1 and thus, explaining the lower content of elements detected in the extract (Na, K and Mg) with the Na:K ratio of 1.79. Even though the Na:K content in this study was slightly higher than recommended by the World Health Organization (WHO) with an optimal Na:K ratio of ~1, nevertheless, the ratio reported in this study was relatively lower than those reported in processed meats, white bread/rolls, and savoury sauces and condiments, with Na:K ratios of 7.8, 6.0, and 5.4, respectively (O'Halloran et al. 2016). Besides that, the two trace elements identified was Mg (6.15 mg L −1 ) and heavy metal, As (0.17 mg L −1 ). The level of the As is within the allowed limits by Food and Drug Administration, 2018 for cosmetic (< 10 mg L −1 ), however slightly higher for consumption (< 0.1 mg L −1 ). Besides that, the toxicity study conducted through the BSLA revealed that the methanol extract was non-toxic with LC 50 of 15.60 mg mL −1 .
Further analysis of the NaAlg extract was conducted (Table 4). Comparatively, in this study, higher NaAlg (20.00%, DW) yield was reported compared to S. wightii (21.71%) and S. myriocystum (20.10%) (Subramanian et al. (2015). Subsequently, the antioxidant study revealed that NaAlg from S. polycystum reported higher antioxidant activities (DPPH = 7.61 ± 2.74 µM TE.g −1 ; FRAP = 34.68 ± 0.23 µM TE.g −1 , ABTS = 34.08 ± 0.10 µM TE.g −1 ) compared to commercial NaAlg (DPPH = 0.57 ± 2.23 µM TE.g −1 ; FRAP = 4.28 ± 1.03µM TE.g −1 , ABTS = 8.60 ± 0.10 µM TE.g −1 ). This could be due to the secondary metabolites impurities such saponins and flavonoids detected through the phytochemical analysis. Marimuthu et al. (2012) have also reported similar impurities in the NaAlg extract of S. wightii. The compounds have a high tolerance level towards various factors such as heat and UV rays, and therefore could be an advantage in various industrial applications as they possess several biological activities such as antimicrobial, antiviral, antioxidant and anticoagulant (Jeyaraman et al. 2013;Cox et al. 2011). Thus, it is worth exploring the synergistic effects of NaAlg and the impurities present as a powerful antioxidant tool. The analysis of NaAlg through the FTIR analysis could be used as a method to identify the presence of NaAlg. Fig. 1 shows the characteristic peaks of NaAlg in the range of 950 to 750 cm −1 with the peak (945.5 cm −1 ) indicating the C-O is stretching vibration of uronic acid and peak (810.67 cm −1 ) assigned to mannuronic acid residues (Fertah et al. 2017). The 1 H-NMR analysis of the extracted NaAlg shown in Fig. 2 indicated an M:G ratio of 0.733 which was similar to other Sargassum species that shows values in the range of 0.94 and 1.07 (Fenoradosoa et al. 2010). However, the similar author reported NaAlg from S. polycystum with a lower M:G (0.21) compared to the NaAlg in this study. It has been previously reported that such discrepancy was evident for S. fluitans and S. oligocystum that showed variation in the M:G ratio depending on the extraction procedures and the origin of the seaweed with values ranging from 0.52 to 0.57 and 0.49 to 0.62, respectively (Torres et al. 2007).
Further increment in the NaAlg samples was performed via UVirradiation and sonication methods. Increasing antioxidant activities (DPPH, FRAP and ABTS) of the treated polysaccharides with increasing treatment time of 15 min to 120 min is shown in Table 4. The FTIR profile, in Fig. 3 shows changes in the absorption band at 3,437 cm −1 (OH groups) where the intensity broadens with irradiation time. Simultaneously, the decreasing peak intensity of 1,603 cm −1 (C-O) and 1,032 cm −1 (C-O-C) was perceived consistent with the random scission of the glycosidic bonds of the NaAlg backbone and the formation of new functional groups, carbonyl and carboxyl groups that enhances the controls the antioxidant behaviour of polysaccharide-type polymers (Choi et al. 2009;Nagasawa et al. 2000). The spectrum analysis of the sonicated NaAlg detected no obvious changes to the functional groups. Wasikiewicz et al. (2005) reported similar findings and suggested that changes in the molecular weight of NaAlg from 2.16 × 10 6 Da to 2.92 × 10 5 Da via sonication undergoes different mechanism than ultraviolet and gamma degradation.

CONCLUSIONS
Overall, the methanol extract and NaAlg from brown seaweeds are commonly extracted and explored by industries for various applications, thus, an overview of the bioactivities and physicochemical analysis of the extracts from S. polycystum could pave future studies on this species from Malaysia. In this study, the crude methanol extract showed the presence of beneficial elements such as Na, K, Mg, vitamin A and vitamin E. In addition to that, due to its low toxicity, high antioxidant activities and antibacterial activities against food pathogen, the extract could be further developed as dietary supplements, food products or cosmetics. Besides that, the effects of UV-irradiation and sonication could be employed as effective methods of improving the antioxidant activities of NaAlg. Generally, at 120 min, the treatment of polysaccharides under sonication showed a higher increment in antioxidant activities compared to UV-irradiation. However, considering the time required for degradation to take effect, the ultraviolet seems to be the better method compared to ultrasonic degradation. However, limitations such as low UV light penetration and its application to a limited depth of the solution may restrict its commercial applicability. Nevertheless, this method deemed more convenient, easy and cost-effective process for the industry. Besides that degraded polysaccharides are more soluble and less viscous and thus, will not alter the texture and the appearance of the products. Thus, at present, our group is exploring in vivo, their cytotoxic activity upon potential application and commercialisation.