Today, plasma is extensively utilized in biomedicine. Given its widespread use, the presence of unknown variables, and potential hazards like electric shock and burns, this research explores the electrical, optical, and chemical aspects of plasma generated through floating electrode dielectric barrier discharge (FE-DBD). The aim is to ensure the device's electrical safety and evaluate its chemical and thermal properties. Additionally, this study investigates the parameters of the produced plasma. It provides an immunometric assessment and characterization of discharge plasma from the floating electrode dielectric barrier, facilitating device optimization and advancing our understanding of plasma characteristics for tailored biomedical applications.