pvSPS4 is Crucial for the Root Sugar Balance and Proper Tolerance Response in Salt-Tolerant Common Bean Genotype Under Salt Stress


 Salinity is a stress factor that decreases global agricultural yield. Crops such as rice, tomato, potato, and legumes are susceptible to salt, thus this problem requires urgent attention. Stress-related carbohydrate metabolism is a dynamic network of genetic and metabolic pathways. Disruption in the regulation of soluble sugar content can have negative effects on source-sink partitioning. As source-sink partitioning is connected to the supply, and distribution of energy in the plant, it is critical for stress tolerance. Sucrose phosphate synthase (SPS) enzymes operate in a key regulatory step in sucrose synthesis. Therefore, they have a significant role in sugar metabolism including the regulation of source-sink sucrose transportation. This study focused on the function of the SPS homolog -pvSPS4- in the roots of legume crop common bean under salinity stress. We previously showed that pvSPS4 expression is root-specific and is upregulated under salt stress in a salt tolerant common bean genotype. This upregulation was accompanied by an accumulation of sugars in the roots. In the current study, using the same genotype, we generated composite common bean plants with wild-type shoot and pvSPS4 knock-down roots. Composite plants exhibited increased sensitivity to salt stress compared to control and mock plants. pvSPS4 knock-down disturbed the root glucose/sucrose ratio and ionic balance which resulted in a reduction in photosynthetic pigments together with osmoregulation and antioxidant capability. Our results imply that pvSPS4 is an important gene for carbohydrate balance regulation as a salt-stress response in the common bean root tissues.


Introduction
Soil salinity is one of the world's most widespread and detrimental agricultural problems. Projections show that more than 20% of farmlands are affected by over-salinization globally (Munns and Tester 2008). This percentage is expected to increase annually due to improper irrigation, disproportionate evaporation, or insu cient precipitation and reach 50% by 2050 (Jamil et al. 2011). Major crops such as maize, potato, rice, tomato, and legumes are susceptible to salinity-related yield loss, thus this issue requires urgent attention (Muchate et al. 2016).
Stress-associated sugar metabolism is a dynamic process that is interconnected with numerous other metabolic and genetic pathways. Variations in CO 2 xation can cause uctuations in soluble carbohydrate content and interrupt the source-to-sink carbon partitioning. Regulation of such impairments requires the expressional adjustment of many genes and proteins (Roitsch 1999; Gupta and Kaur 2005). As source-sink partitioning is directly connected to energy and resource allocation throughout the plant, it is a crucial factor in stress tolerance (Ho 1988; Krapp and Stitt 1995). Additionally, soluble sugars act as primary messengers and take a role in the regulation of growth and development-related genes, thus disruption of carbon partitioning Common bean (Phaseolus vulgaris L.) is a salt-susceptible major legume crop that can suffer around 20% loss in yield even at low salinity levels (Chinnusamy et al. 2005). Since it represents nearly half of global legume production, such a drop in yield can lead to problems with the nutrition of a high percentage of the human population (Broughton et al. 2003).
Our previous omics study compared two common bean genotypes to understand the divergence in their salt-tolerance responses. This revealed a major distinction in carbohydrate metabolism between these genotypes: The tolerant genotype Ispir accumulated greater levels of soluble carbohydrates in the roots compared to the susceptible genotype (TR43477). Together with this accumulation, a sucrose phosphate synthase (SPS) gene displayed a sharp upregulation in expression in the tolerant genotype that is absent in the susceptible genotype (Niron et al. 2020).
SPS enzymes act in the rst step of the sucrose synthesis pathway with the transfer of a glycosyl group from an activated sugar donor such as uridine diphosphate glucose to an acceptor fructose-6'-phosphate to generate sucrose-6'-phosphate (Chua et al. 2008). As a rate-limiting step, this reaction constitutes a crucial regulatory phase for the synthesis of sucrose (Stitt et al. 1988; Huber and Huber 1996). Therefore, SPS enzymes have a key role in carbohydrate metabolism with the adjustment of partitioning among starch production and soluble sugar accumulation in several physiological systems (Geigenberger et al. 1999;Lunn and MacRae 2003;Baxter et al. 2003; Chen et al. 2005). Furthermore, they are tightly connected with the level of source-to-sink sucrose translocation (Huber and Huber 1992) and since nearly 80% of xed carbon is exported to sink tissues (Kalt-Torres and Huber 1987), SPS regulation of partitioning is critical for control growth and development (Gifford et al. 1984).
There are multiple SPS homologs in plant species, and their transcriptional regulation depends on developmental stage, tissue type, and environmental stimuli (Reimholz et al. 1997;Lunn 2003;Lut yya et al. 2007). SPS activity may display an increase or decrease in response to salinity stress depending on the genotype or species (Amirjani 2010;Peng et al. 2016). Similarly, SPS activity was demonstrated to increase in drought-stressed wheat (Fresneau et al. 2007), and rice leaves (Xu et al. 2015), yet a recent study of soybean under drought stress showed increasing and decreasing patterns of expression depending on developmental stage, tissue type, and SPS homolog (Du et al. 2020). Another recent study on cold-stressed maize plants also demonstrated the genotype effect on SPS regulation (Bilska-Kos et al. 2020). The range of variability in SPS expression and protein activity indicates a comprehensive regulatory function for this enzyme family, thus further attention is necessary to enhance our knowledge of the role of SPS enzymes not only in plant metabolism but also in stress tolerance.
There is a large body of research on the role of SPS genes in photosynthetic tissues, hence much of the knowledge of its function and regulation is related to these tissues (Huber and Huber 1996;Lunn and MacRae 2003;Baxter et al. 2003). On the other hand, the function of SPS enzymes in non-photosynthetic tissues, where sucrose is imported and degraded, is not yet clear. SPS may have two roles in non-photosynthetic tissues: It may take part in sucrose re-synthesis subsequent to import through apoplastic cleavage or it may be directly involved in the sugar regulatory cycle with the organization of sucrose and/or starch balance (Geigenberger et al. 1999).
In this study, we have generated composite common bean plants with Ispir genotype (salt-tolerant), through Agrobacterium rhizogenes mediated transformation, that have SPS4 (pvSPS4) knock-down roots and wild-type shoots to understand the role of a root-speci c, salt stress-responsive SPS enzyme in salt stress tolerance. Root pvSPS4 knock-down disturbed the glucose/sucrose ratio in root tissues which caused ionic imbalance and reduced carbon xation. Knock-down plants displayed reduced growth, higher cell death, and decreased osmoprotection and antioxidant capacity. Here we report that pvSPS4, through the regulation of sugar balance, acts as a crucial salt-stress response actor in the root tissues of common bean.

Methods
Bacterial strain, plasmid, and vector construction Part of the human ferritin (EC:1.16.3.1) gene was selected to be cloned as a mock control, to invoke the RNAi system in common bean roots in a nonselective manner. 280bp and 268bp fragments for common bean pvSPS4 (Phvul.005G002600) and human ferritin genes respectively were ampli ed (Table 1) to be inserted into the pK7GWIWG2_II-RedRoot RNAi Gateway vector. The vector consists of an intron-linker between insertions to produce an intron-containing hairpin RNA (ihpRNA) for the use of host RNAi machinery together with a dsRED reporter linked to a 35S promoter. Obtained expression vectors were transformed to A. rhizogenes K599 strain.
Composite plant production, plant growth, stress treatment Three separate lines of composite plants with wild-type shoots and transgenic hairy-roots were created for comparative analysis: Blank, mock and pvSPS4 knock-down. Blank plants had hairy roots without any construct; mock plants (HumFer thereafter) had hairy roots with human ferritin-ihpRNA construct and plants with pvSPS4-ihpRNA construct carrying hairy-roots (SucPho thereafter). Five independent (from separately transformed individual common bean plants) composite plants were generated and grown as biological replicates for each line and condition.
Production of transgenic hairy-roots on common bean (Ispir genotype) plantlets was performed as described by Estrada-  3 ) and grown for about 5 days until the emergence of cotyledons. At this point, the pods were soaked with full-strength B&D (supplemented with 8mM KNO 3 ) and hairy-root transformation for the selected genes was performed. For the growth of hairy roots, the pods were transferred to transparent containers that ensured a humidity level above 95%. The leaves were sprayed with B&D (supplemented with 8mM NO 3 ) every two to three days to keep the humidity levels high.
After 18-21 days post-transformation, primary roots were removed by excision approximately one cm below the hairy root emergence site. Plants with transgenic hairy roots were transferred to bigger (12cm diameter) pots with B&D (supplemented 8mM KNO 3 ) saturated vermiculite, the leaves were sprayed, and the plants were grown in transparent containers for another week to facilitate root growth, before removing the lid of the containers. Plants were irrigated with half-strength B&D (supplemented with 2mM KNO 3 ) and grown for another week. After this point, control plants were grown in optimal conditions. Salt treatment was performed in line with our previous study to obtain compatible results: The plants were subjected to gradual NaCl treatment starting with 50mM rst day, increased to 100mM on the second day, and set to 125mM on the third day. In total, the plants were grown under 125mM NaCl for three days before they were sacri ced for tissue sample collection.
The employed methodology for composite plant generation introduced stress factors before salt application: Excision of primary root and high levels of humidity. As a result, most of the cotyledons fell off or in a necrotic phase before the start of salt treatment, therefore they were not used as samples in this study. Five plants were grown as biological replicates for each line and condition.

Measurement of gene expression and protein levels
Total RNA extractions for RT-qPCR analysis were performed with 0.1g homogenized root tissue, using Tri Reagent© (Sigma, US) according to the manufacturer's instructions. Quanti cation and quality of the RNA samples was determined both spectrophotometrically (Barbas et al. 2007) and using 1% denaturing agarose gel electrophoresis. RT-qPCR was carried out with primers for pvSPS4 and its two close homologs, pvSPS1 and pvSPS3 ( Table 1) with 10ng of cDNA from the hairy roots. Three biological replicates with experimental triplicates were used for analysis. PikoReal 96 Real-time PCR system (Thermo Fisher Scienti c, DE) was utilized for the experiment. Actin-11 (GenBank: CV529679.1) was used as the reference gene. Relative

Physiological parameters
Leaf area quanti cation was performed manually with ImageJ software (Schneider et al., 2012) using three leaves from each plant. Fresh, turgor, and dry weight measurements were performed for ve 1.5cm diameter leaf discs and relative water contents (RWC) were calculated using the formula given below: (Barrs and Weatherley 1962). Leaf discs were incubated in ddH 2 O for 4h for the determination of turgor weights. Dry weight measurements were taken following overnight incubation in 80°C oven.
Electrolyte leakage assay was performed with ve 1.0cm diameter leaf sections. Increase in the electrical conductivity of the water was measured with a conductivity meter at 2nd, 4th, and 8th hours after sectioning (Hatsugai and Katagiri 2018).

Molecular parameters
Leaf and root proline contents were determined using the acidic ninhydrin colorimetric assay (Bates et al. 1973). Proline concentrations were calculated as described in Abrahám et al. (2010) with 0.1g homogenized tissue sample. The absorbance (A) was measured at 520nm using toluene as reference. A standard curve with A 520 values of proline standard (5-500μg/ml) was used to calculate the concentration of the samples.
Leaf chlorophyll content was determined following extraction of leaf tissue (0.1g) in methanol as described by Warren (2008). A 652 , A 665, and A 470 were measured using methanol as reference.
Crude protein extraction for antioxidant enzyme activity measurements was performed with 0.1g ground leaf tissue. Fresh Tissues were homogenized in 1ml of ice-cold 100mM PBS (pH 7.8 with 0.1mM EDTA) and the supernatant was collected after 20min centrifugation at 13.000g, 4°C. The concentration of the crude protein extract was spectrophotometrically calculated using the Warburg-Christian formula (Simonian and Smith 2001).
Catalase (CAT) activity was determined as described by Chen and Zhang (2016) by measuring the change in OD 240 in 50μl crude extract every 10sec for 2min. Ascorbate peroxidase (APX) activity was determined as described by Elavarthi and Martin (2010) by measuring the change in OD 290 in 90μl crude extract due to ascorbate oxidation of in the reaction every 15sec for 3min . The basal level of ascorbate oxidation was measured using PBS (pH 7.0) instead of crude extract.

Ion contents
Ground root and leaf tissue samples were oven-dried at 80°C. Tissue samples (0.1g) were mixed with 10ml HNO 3 and 5ml H 2 O 2 .
The digestion was achieved by heating the samples 10min at 100°C, then 15min at 150°C, and nally 15min at 180°C. Solution volume was adjusted to 25ml with dH 2 O. Sodium, calcium, potassium, and magnesium ion concentrations were measured by inductively coupled atomic emission spectrometry (Agilent 700 Series ICP-OES, Agilent, US).

Statistical analysis and correlation
Statistical analyses were performed with Graphpad Prism version 9.1.2 for Windows (GraphPad Software, San Diego, California USA, "www.graphpad.com"). RT-qPCR for pvSPS4 knock-down in different lines was analyzed with One-Way Anova (Brown-Forsythe and Welch test) and multiple comparisons were performed with Dunnet's T3. Data of RT-qPCR for SPS homologs and WB were analyzed with parametric t-test due to the relative nature of measurements. Analysis of other physiological, molecular and ion content measurements were performed with 2-Way-Anova. Multiple comparisons for control vs treatment conditions were implemented with Sidak's test and displayed with black outlines. Control vs control and treatment vs treatment comparisons between lines were done with Tukey's test: Control comparisons were displayed with blue outlines and treatment comparisons were displayed with ruby outlines (* -p<0.05, ** -p<0.01, *** -p<0.005, **** -p<0.001). 2-Way-Anova summary results were given in Table S1, S2 and S3. Correlations of the responses of the lines to salt stress was assessed with Spearman's Rank Order correlation.

Results
Knock-down of root pvSPS4 does not induce high expression of other SPS homologs The main methodology of this study, A.rhizogenes mediated transformation, has a highly variable e ciency that depends on the species of interest and the approach that is employed ( RT-qPCR analysis con rmed the knock-down of pvSPS4 gene under treatment conditions in SucPho roots while the Blank and HumFer plants roots have displayed an upregulation pattern for pvSPS4 under salt stress as expected (Fig. 1e) Under control conditions, pvSPS4 expression did not alter for either HumFer or SucPho roots (Fig S1). WB analysis demonstrated stable control and treatment pvSPS4 levels in Blank and SucPho roots. The knock-down caused a 39.24% decrease (p < 0.01) in pvSPS4 protein levels for the SucPho roots under salt treatment conditions. No signi cant difference was observed for pvSPS4 levels in control conditions among lines (Fig. 1f).
All organisms are able to endure genetic modi cations and disruptions and prolong their tness through genetic robustness. This capability can be due to genetic redundancy which is the compensation for the inadequacy of one gene by an alternative with analogous function (Tautz 1992). Although there are nine SPS homologs in the P.vulgaris genome, only two of them, Phvul.003G170100 (pvSPS1) and Phvul.006G031700 (pvSPS3), possess high amino acid sequence similarity (54% and 57% respectively) with pvSPS4. The transcriptome analysis in our previous study has demonstrated that pvSPS4 and its homolog pvSPS1 have root-speci c expression patterns with high transcription rates. However, pvSPS1 presented stable expression under control conditions and salinity stress, unlike pvSPS4. The other SPS homolog, pvSPS3, had very low transcript levels in wild-type root tissues, but a high level of expression in leaves. pvSPS3 also displayed a upregulation in the leaves upon salt stress which indicates a leaf-speci c regulation for this gene (Niron et al. 2020).
The effect of pvSPS4 knock-down on the mentioned SPS homologs, in the hairy roots, was rst analyzed with RT-qPCR to investigate their potential for compensation of knock-down by an upregulation. Knock-down of pvSPS4 did not induce differential expression of pvSPS1 upon salt treatment. In response to salinity, pvSPS3 demonstrated a signi cant downregulation (Fig. 2a). Since pvSPS1 is the root-speci c homolog, we measured pvSPS1 protein levels with Elisa. The pvSPS1 protein displayed a small but signi cant increase (approx. 1.3-fold) in SucPho roots in control, but there was no further increase in treatment conditions (Fig. 2b). Also, SucPho roots displayed higher pvSPS1 protein levels under salt stress compared to HumFer but not Blank roots. Anova results for ELISA (Table S1) demonstrated that the increase of pvSPS1 amount in SucPho roots was signi cantly related to knock-down but not salt treatment. pvSPS4 knock-down changes the root glucose/sucrose ratio in both control and salt-stress conditions As SPS genes are associated with the regulation of carbohydrate metabolism and its partitioning, we investigated the effect of pvSPS4 knock-down on root systems glucose and sucrose contents (Fig. 3). The only signi cant change in glucose levels was a doubling of glucose levels in SucPho roots which decreased to control levels under salt stress. There was no effect on sucrose levels (Fig. 3a). Anova results demonstrated that knock-down, salt treatment and their interaction had signi cant effects on the divergence in glucose levels (Table S1).
The divergence in glucose amount has re ected on the glucose/sucrose ratio in SucPho roots as it displayed a signi cant increase in the control and a signi cant decrease under salt stress compared to Blank and HumFer roots indicated an imbalance of carbohydrate partitioning (Fig. 3b).
Changes in physiological parameters resulting from pvSPS4 knock-down To understand the effects of pvSPS4 knock-down related root-sugar imbalance on carbon xation and plant physiology, we examined the chlorophyll content (Fig. 4) surface area, water content, and membrane integrity of leaves (Fig. 5).
There was no signi cant difference between the control levels of chl a, or chl b among the composite lines. Upon salt treatment, only SucPho plants showed a signi cant content reduction in both pigments, but much higher level in chl b. In treatment conditions SucPho chlorophyll levels were also signi cantly lower than Blank but not HumFer plants ( Fig. 4a-b). Anova results showed that while both pigments were signi cantly affected by knock-down and salt treatment, but only chl b was affected by the interaction of both (Table S1).
The Blank, HumFer, and SucPho lines presented similar phenotypes in control conditions, with a slight growth reduction observed in SucPho plants (Fig. 5a). A larger impact on growth was seen under salt stress: While the Blank plants appeared relatively healthy, SucPho plants were wilted and displayed signs of tissue necrosis. Performance of HumFer plants under salt stress, on the other hand, appeared to be in between the Blank and SucPho plants with observable indications of stress (Fig. 5a).
The reduced growth of SucPho plants was apparent with a 45% reduction in leaf area as well while the loss was only minor for Blank and HumFer plants (Fig. 5b-c). Moreover, SucPho plants have demonstrated a major, 11%, loss in leaf RWC under saline conditions while this loss was about 4% for both Blank and HumFer plants. (Fig. 5d). The effect of knock-down was not signi cant alone according to Anova results, yet the interaction of it with salt treatment was a signi cant player for the observed decrease (Table S2).
The leaf area and RWC losses under salt stress in the case of pvSPS4 knock-down suggested high cellular damage for leaf tissues of SucPho plants. Indeed, salt stressed SucPho leaf sections lost signi cantly more electrolytes on the 4th hour of the sectioning compared to Blank and HumFer leaf sections. On the 8th hour, the only signi cant difference was observed between Blank and SucPho (Fig. 5e). On the contrary, pvSPS4 knock-down did not produce a high electrolyte leakage in control conditions ( Fig S2).
Sugar imbalance had negative effects on the ionic balance of the knock-down plants Next, we have examined the effects of pvSPS4 knock-down related sugar imbalance on ion uptake, transport, and sodium accumulation by measuring, K + , Ca +2 , Mg +2 , and Na + contents of the root (Fig. 6) and leaf tissues (Fig. 7). pvSPS4 knock-down had a major effect on both uptake and transport of the K + : Blank and HumFer root K + levels acted in concert with previous results for Ispir genotype (Niron et. al. 2020) and dropped signi cantly under salt stress while SucPho root K + levels signi cantly increased (Fig. 6a) as observed in a salt susceptible genotype (Niron et. al. 2020). Moreover, the control K + content in the roots was signi cantly higher for Blank and HumFer than SucPho, but it was signi cantly lower in treatment conditions and interaction of knock-down and treatment was the major source for this variation (Table S3). Leaf tissues of all plants have reacted in a similar fashion with a signi cant accumulation of K + in response to salt stress, but the extent of change was different: K + levels increased by 1.25 and 1.35-fold for Blank and HumFer roots respectively while the increase was 1.94-fold for SucPho plants (Fig. 7a).
In general, pvSPS4 knock-down has caused an imbalance in Ca +2 and Mg +2 contents in the tissues. Salt treated SucPho plants presented a signi cant decrease in Ca +2 content in both root and leaf tissues but there was no signi cant difference between the control and the treatment conditions in Blank and HumFer tissues ( Fig. 6b and Fig. 7b). Salt treatment has resulted in a 1.97-fold decrease in SucPho roots while there was no signi cant difference in Blank and Humfer roots. In control conditions, both Blank and HumFer roots displayed signi cantly lower levels of Ca +2 . In treatment conditions, on the other hand, only Blank roots maintained signi cantly higher levels of Ca +2 (1.59-fold) than SucPho roots (Fig. 6b). In the leaf tissues of SucPho plants, Ca +2 levels were much higher than Blank and Humfer plants (1.81 and 2.16-fold respectively) in control conditions. Salinity elicited a steep decrease that has brought SucPho leaf Ca +2 to similar levels of HumFer, yet Blank leaves had signi cantly higher Ca +2 content in treatment conditions compared to others (Fig. 7b). For both tissues, interaction of salt treatment and knock-down was the major source of the variation observed (Table S3).
Blank and HumFer roots did not show a signi cant change in Mg +2 levels under saline conditions, but SucPho roots presented a signi cant decline (2.77-fold). Anova results displayed knock-down as the main source of variation here (Table S3). And, even though control Mg +2 levels were similar for roots of all composite lines, Blank roots have accumulated signi cantly more Mg +2 compared to both HumFer and SucPho roots (Fig. 6c). Leaf Mg +2 contents displayed a similar response as Ca +2 : Blank and HumFer leaf Mg +2 levels did not show a signi cant change upon salt stress. Although SucPho leaf Mg +2 content in control was lower than both Blank and Humfer to begin with, when exposed to stress conditions, it displayed a steep 1.69-fold increase. The SucPho leaf Mg +2 control level was signi cantly lower compared to Blank and HumFer leaves while it was signi cantly higher in treatment conditions (Fig. 7c). In contrast to roots, interaction of knock-down with salt treatment was the main source of variation for leaf Mg +2 levels (Table S3).
All plants presented a majorly signi cant Na + upsurge in the case of salt treatment and there was no signi cant difference between the control or treatment levels of tissues ( Fig. 6d and Fig. 7d). On the other hand, roots displayed a meaningful difference for salt-related Na + uptake regulation in the case of pvSPS4 knock-down: SucPho roots accumulated signi cantly higher Na + compared to Blank and HumFer roots under saline conditions. Interestingly HumFer roots accumulated even lower amount of Na + than Blank roots (Fig. 6d).

Osmoprotection and antioxidant capacity have dropped for SucPho plants
To interpret the physiological consequences of root pvSPS4 knock-down on the tissues, we examined the changes in osmoprotection and antioxidant capacities of the composite lines (Fig. 8). Both systems are known to be highly salt stressresponsive, and a decrease in capacity signi es the increase in sensitivity to stress (reviewed in Parida and Das 2005). Content analysis of proline, a key osmoprotectant (Hayat et al. 2012), in leaves and roots helped us infer the osmoprotection capability of the composite lines in response to salt stress. Curiously, leaf tissues of all lines accumulated signi cantly different levels of proline from each other in control conditions. Upon salt stress, all composite lines managed to signi cantly increase their proline content, but to different extents. Blank leaf proline levels have increased by 17.95-fold, while HumFer leaf proline levels have displayed a 3.51-fold upsurge; for SucPho leaves the increase was only 1.63-fold (Fig. 8a).
For root tissues in control conditions, Blank roots accumulated a signi cantly higher amount of proline compared to HumFer and SucPho roots. Nonetheless, the root proline levels of Blank and HumFer plants displayed a moderate but signi cant increase (1.91 and 2.01-fold respectively) as a salt stress response, but such increase was absent in SucPho roots (Fig. 8b).
A perturbed antioxidant removal capacity for leaves of the SucPho plants was observed under salt stress (Fig. 8c, d). In control conditions, pvSPS4 knock-down did not cause a signi cant change in leaf APX activity but SucPho leaves could not manage to increase APX activity under stress conditions as well. On the other hand, APX activity of Blank and HumFer leaves increased by 4.77 and 7.95-fold respectively (Fig. 8c). Similarly, SucPho leaves did not demonstrate any escalation in CAT activity in saline conditions in contrast to Blank leaves which presented more than a 1.5-fold increase, but such response was also absent in HumFer plants as well.
The correlation of responses was higher for Blank and HumFer than SucPho Although most of the physiological and molecular responses of Blank and HumFer plants to salt stress were similar to each other and deviated from the responses of SucPho plants, there were noteworthy differences between them in control and treatment conditions when compared separately. The adopted methodology introduced another variable, separate from the salt stress application, with the activation of RNAi machinery. Thus, we mapped all the results for the salt stress response of the lines together in the log 2 scale to visualize the variation among them (Fig. 9a). Responses of the Blank (black) and HumFer (yellow) plants presented a highly overlapping pattern compared to SucPho (red) in the map. Moreover, we performed a correlation analysis to measure the resemblance of the response patterns of the lines (Fig. 9b). Responses of Blank and HumFer plants displayed very high correlation with Spearman r-value of 0.95. On the other hand, root pvSPS4 knock-down caused a major decrease in correlation level. These results demonstrated the extent of the knock-down of the gene over the in uence of RNAi machinery on the stress response.

Discussion
Soil salinity has become a global crisis that causes many adverse effects on crop development and productivity. Salinity generates intricate problems that disturb nutrient uptake, and general metabolism that result in decreased growth rate. These Sucrose resynthesis in the roots by the activity of sucrose metabolism enzymes such as SPS is deemed to be essential for its storage or intercellular transport. Sink SPS metabolism has been linked with protein storage (Weber et al. 1997), starch accumulation (Cheng and Chourey 1999), cellulose production (Babb and Haigler 2001), and it can be crucial for proper regulation of carbohydrate partitioning (Cheng and Chourey 1999). However, molecular evidence about sucrose resynthesis and the role of SPS activity in sinks, particularly under stress conditions, is still limited. To address this issue, in this study, we have investigated the effect of the knock-down of root-speci c, salt-responsive common bean SPS homolog, pvSPS4, under salt stress.
We have utilized A.rhizogenes mediated hairy root induction to generate composite plants with pvSPS4 knock-down through RNAi. Two experimental control lines were constructed to compare the effects of pvSPS4 knock-down: Blank line with no vector insertion to use as the main background, and HumFer line with RNAi triggered to function as a background purely to evaluate the outcomes of RNAi activity. RNAi off-target effects can arise when short interfering RNAs (siRNAs) alter the expression of genes different from their target (Birmingham et al. 2006;Jackson 2006). There is cumulating evidence that some of these off-target effects are result of siRNAs operating as microRNAs (miRNAs), targeting mRNAs that include partial sequence matches to the siRNAs (Saxena et al. 2003;Birmingham et al. 2006;Aleman et al. 2007). In this regard, the observed phenotype dissimilarities between HumFer and Blank lines are reasonable since in HumFer plants, the RNAi machinery is active. Nonetheless, SucPho line was demonstrated to have signi cant complications on the salt tolerance mechanism, compared to both HumFer and Blank, by correlation analysis (Fig. 9). pvSPS4 may regulate ionic balance through the adjustment of sugar balance in the roots Though pvSPS4 was upregulated under stress conditions for Blank and HumFer roots, the protein levels were stable. But SucPho roots displayed a nearly 40% reduction in pvSPS4 protein content under salt treatment implying a high turnover rate for this enzyme in such conditions. pvSPS4 knock-down resulted in pvSPS3 downregulation under treatment conditions in the roots and increase in pvSPS1 protein content in both conditions. Although we did not observe signi cant decrease in transcription or protein content of pvSPS4 in control conditions, we might conclude that knock-down caused a problem in SPS enzyme balance in the root tissues which might be the main source of differences observed in SucPho plants in control conditions. pvSPS4 knock-down caused an overall 1.92-fold higher glucose accumulation in control conditions in the roots, yet the sucrose levels did not change. On the other hand, there was no signi cant difference in glucose or sucrose levels among the lines in treatment conditions. Nonetheless, the differences between the contents have re ected on the glucose/sucrose ratio of the lines.
The imbalance was apparent in SucPho lines with signi cant disparity in control condition (Fig. 3b). The change in this ratio for SucPho roots was overall 2.71 times higher than the roots of other lines upon salt treatment. Interestingly, in our previous study, a similar difference was observed between the roots of the salt-tolerant Ispir and salt-susceptible TR43477 genotypes, with the latter displaying a 2.35 times greater decrease under salt-stress (Niron et al. 2020). The similarity of this variation suggests that it can be a key subject for the salt tolerance response. Since these sugars have major and independent roles in regulatory In turn, these sugar-sensing pathways can affect the sucrose metabolism (Koch et al. 1992;Roitsch et al. 1995;Stitt and Hurry 2002), creating a feedback loop. Therefore, it is reasonable to deduce that balance adjustment of these sugars is crucial for a decent stress response.
The pvSPS4 knock-down also resulted in an ionic imbalance in leaf and root tissues under control conditions, which was intensi ed under salt stress when compared to the other two lines. The imbalance was apparent for K + in SucPho roots (Fig. 6a), there was a steep contrast in Ca +2 content for both conditions in both tissues ( Fig. 6b and Fig. 7b) and SucPho plants could not manage to balance Mg +2 levels in the leaves (Fig. 7c). However, this imbalance did not yield a major difference in Na + accumulation for SucPho plants. Although SucPho roots accumulated signi cantly higher Na + , this did not affect the leaf Na + levels ( Fig. 6d and Fig. 7d).
There are several routes that a glucose/sucrose imbalance can be linked to ionic imbalance: Both sucrose and glucose function as osmolytes and maintain homeostasis in the cell (Gupta and Kaur 2005), yet sucrose is much more common and crucial in osmoregulation than glucose (Slama et al. 2015). Furthermore, both sugars were demonstrated to regulate membrane potential in different extents (Franz and Tattar 1981), and both K + and Ca +2 uptake are strongly related to membrane polarization status of the cell (Geiger et al. 1998;Miedema et al. 2001Miedema et al. , 2008. Notably, K + ions have to move against their concentration gradient to enter the root. To make this movement energetically favorable, the activity of H + -ATPases, which are displayed to be induced by sucrose (Mito et al. 1996) are required (Nieves-Cordones et al. 2014). Thus, a lack of balance between glucose and sucrose in the root tissues might have disrupted the maintenance of cellular osmolarity, membrane potential, and H + gradient, especially in the case of salt stress, and resulted in dysregulation of ion uptake mechanisms in common bean.
Another potential route for glucose/sucrose imbalance to cause the ionic disorder is the disruption of the expressional regulation of circadian clock genes. Studies have displayed the connections between the circadian clock, immunity, and the sugars in plants (Roden and Ingle 2009; Bolouri Moghaddam and Van den Ende 2013). The clock genes were found to be in strong interplay with sucrose levels and invertases -enzymes that catalyze sucrose to hexose -which indicates that the ne regulation of hexose/sucrose ratio is vital for an appropriate stress-response (Haydon et al. 2013). Furthermore, a properly functioning clock is necessary for the regulation of cellular auxin levels and response to it in the root tissues (Voß et al. 2015). Auxin is a potent inducer of K + uptake through the regulation of H + pumps (Marten et al. 1991;Lohse and Hedrich 1992) and it possesses a crucial role in the intercellular Ca +2 and K + transport and Ca +2 signaling through cation channel activation (Philippar et al. 1999;Shishova and Lindberg 2004;Kirpichnikova et al. 2014). Therefore, the disproportion in SucPho root K + and Ca +2 contents in both control and salt treatment conditions can be due to disturbance of auxin regulation through the circadian clock. Moreover, Ca +2 content and signaling were shown to be vital in modifying the selectivity and absorption of other ions, including magnesium (Tang and Luan 2017), for proper salt tolerance in various plants (Epstein 1961;Rains and Epstein 1967;Zhu 2002) which might explain the signi cant changes in Mg +2 levels for SucPho plants upon treatment.
Transmission and effect of ionic imbalance to the leaves The plant circadian clock of different organs has been demonstrated to be dependent (James et al. 2008) so, any disruption of the clock through the glucose/sucrose imbalance in one part of the plant can be expected to affect other parts as well. Moreover, any interruption in Ca +2 signaling holds potential to have major negative effects on K + levels and transport (Ivashikina et al. 2005;Cheong et al. 2007;Lan et al. 2011;Ren et al. 2013) which is tightly coupled with the regulation of source to sink nutrient transportation including sucrose (Geiger et al. 1998). Hence, a disturbance in the root circadian clock together with Ca +2 signaling may have been the main reason for the transmission of resulting complications to the upper parts of the plant.
The disruption of ionic regulation and possibly shoot-to-root transport had resulted in an impaired salt stress response for the leaf tissues in SucPho plants. Leaves displayed reduced chlorophyll content (Fig. 4) and compromised physiology (Fig. 5). As our previous study implied, preservation of chlorophyll levels is crucial for an effective salt tolerance response (Niron et al. 2020), so understanding the mechanism that caused chlorophyll decrease in SucPho leaves upon salt stress is critical to interpret other physiological consequences. Leaf Ca +2 and Mg +2 levels presented both conditional and directional contrasts for SucPho plants ( Fig. 7b and c). Mg +2 is a major element in chlorophyll metabolism (Masuda 2008) and takes role in Rubisco activation (Portis and Jr. 2003). An uneven rise in the Mg +2 content might have been responsible for the disruptions in chlorophyll synthesis in SucPho leaves. Furthermore, it might have had negative effects on the TCA cycle and amino acid synthesis as well (Kwon et al. 2019). Ca +2 also holds key roles in photosynthesis (Wang et al. 2019), energy production (Stael et al. 2012) and regulation of salt stress responses in plants (Manishankar et al. 2018). Therefore, the combined effect of Mg +2 and Ca +2 imbalance in the leaves has potential to cause the growth reduction as implied by leaf area measurements (Fig. 5a, b, and c) and interrupt a proper salt stress response in SucPho plants.
Moreover, leaf and root tissues of SucPho plants, in contrast to Blank and HumFer plants, could not manage to accumulate the major osmoprotectant proline (Hayat et al. 2012) in response to salt stress (Fig. 8a, b) which suggested a turmoil in osmoregulation capacity for SucPho. Indeed, the leaves of SucPho plants lost signi cantly more water compared to other lines (Fig. 5d). This problem might be caused by the above-mentioned disruptions in photosynthesis and energy metabolism together with the effect of Mg +2 accumulation on amino-acid synthesis. Another suspect for the inadequacy in proline production can be the disruption of Ca +2 signaling which is known to play a major role in the regulation of proline accumulation upon ionic stress conditions (Parre et al. 2007).
Salt stress, in addition to osmotic and ionic factors, induces oxidative stress with a rise in reactive oxygen species (ROS) (de Azevedo Neto et al. 2006). A decline in water content leads to stomatal closure and a related drop in CO 2 content, a vital electron acceptor, which then results in the over-production of ROS (Noctor 2002;Laxa et al. 2019). As SucPho leaves experienced a higher rate of RWC loss in stress conditions compared to Blank and HumFer lines (Fig. 5d), it is safe to presume that they also produced more ROS as well. Yet, the oxidative stress did not evoke a strong APX (Fig. 8c)  In conclusion, pvSPS4 knock-down in the roots of common bean has disrupted the glucose/sucrose balance in the root tissues which in turn obstructed the constitution of ionic balance under salt stress. K + and Ca +2 imbalances in the root system possibly had negative outcomes in the root-to-shoot nutrient transport system and invoked a Ca +2 and Mg +2 imbalance in the leaf tissues.
The adverse effects of ionic disproportion together with declined water availability in the leaves, distorted the photosynthesis and possibly energy production metabolism; reduced the growth, and resulted in increased cellular damage due to interruption of osmoprotection and antioxidant removal machinery. Considering the extent of salt susceptibility which the knock-down of the pvSPS4 caused, it is safe to say that this sugar metabolism gene holds a critical role in salt-tolerance pathways and deserves attention in the future development of salt-tolerant varieties.

Declarations
Author Contribution HN and MT conceived and designed the research. HN conducted the experiments and data collection (except for ICP-OES measurements). ICP-OES measurements were conducted by Yildiz Technical University Merklab. HN performed the statistical analysis of the data. HN and MT wrote the manuscript.  Tables S1-s3   Table S1. Anova summary statistics for molecular measurements.   Evaluation of K + (a), Ca +2 (b), Mg +2 (c), and Na + (d) content changes in the leaf tissues of plants. All plants accumulated K + under treatment yet SucPho control and treatment levels were signi cantly different than others (b). A major imbalance was observed for Ca +2 (b) and Mg +2 contents (c) for SucPho leaves. Na + accumulation did not show a signi cant difference among plants (g) (n = 5). Values represent the mean ± SEM. (ppt -parts per thousand) Figure 8 Examination of osmoprotection -proline content (a, b)-and antioxidant removal -APX (c) and CAT (d) activity -capabilities of plants. All plants accumulated proline in leaves but in signi cantly different levels (a). In the roots, Blank and HumFer managed to signi cantly increase its proline content upon salt stress (b). SucPho leaves did not present any escalation in APX (c) or CAT (d) activity upon treatment, increase in CAT activity was absent in HumFer as well (n = 5). Values represent the mean ± SEM Figure 9 Evaluation and comparison of the responses Blank, HumFer, and SucPho gave to salt-stress. Comparison of the log 2 foldchanges (a) displayed the disparity of the reactions SucPho (red) gave relative to Blank (black) and HumFer (yellow). Spearmanr correlation of log 2 fold-changes (b) has shown the distinctions of the plants

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