AN EPISTEMOLOGICAL BACKGROUND ON PARADIGM FORMATION OF LIPIDOMICS OF DAIRY INDUSTRY

The article considers post-genomic view on formation of scientific ideas about one of the major components of raw milk and products produced from it (for example, cheese). It is a lipid complex (milk fat) of the brand Lipidomics. The cluster structure of milk fat, its components and derivatives are described. The author shows the dynamics of milk fat transformation in the process of cheese production in logistic link to lipolytic activity of bacterial starters and enzyme preparations. The characteristic of lipid complex of milk whey is given. Information allows us to formulate Lipidomics positions of dairy products. The aim of the article is to attract researchers’ attention to the object, and practitioners to a rational and careful use of milk fat in food products.


INTRODUCTION
Formulation of the problem. According to the principles of Lactoomics [1] (science about milk) and logistics of dairy industry [2], at the present level of knowledge [3,4] Lipidomics of milk and dairy products is reduced to milk fat -monitoring of composition and properties, cooking and use. The perfect model is the production of butter [5] and spreads [6]. This subject is fully and systematically covered by works of Franz Adamovich Vyshemirsky, Doctor of Technical Sciences, Professor, Laureate of the State Prize of the Russian Federation, by his students and followers. There are more than 1.000 publications on this topic which give all the necessary information about the subject -milk fat, butter-making, dairies and a wonderful indispensable productbutter [7] -which is the indicator of dignity and wellbeing of human civilization.
Based on post-genomic biotechnology view on concepts of objects and phenomena knowledge, taking into account the experience of Glikoomics formation [8], it is useful to formulate epistemologically and as a paradigm our vision of one of the major components of milk -the fat phase -Lipidomics. The starting material and the base model of the article are numerous researches of Professor M.S. Umansky [9]. This article is dedicated to his blessed memory.

OBJECTS AND METHODS OF STUDY
The object under review is the unified scheme of milk lipids classification by M.S. Umansky, which is shown in Fig. 1.
The basic problem-target structure of research of raw milk lipids by M.S. Umansky with the interpretation of the whole range of milk and milk products, at the example of cheese-making is shown in Fig. 2.
Taking into account the lack or absence of identification procedures for simulation of milk lipids and its products, as well as bacterial cultures and enzymes, M.S. Umansky developed and modified 17 original methods of studying the objects of knowledge. A list of some of the information is provided: -preparative TLC analysis of N-lipid; -micro TLC analysis of N-lipid; -preparative TLC analysis of P-lipid; -Micro-TLC analysis of P-lipid; -GLC analysis of fatty acid composition; -GLC analysis of free fatty acids; -GLC analysis of volatile fatty acids; -esterase activity of mesophilic and thermophilic lactic acid bacteria; -esterase activity of propionic acid bacteria. Fig. 3 shows the logistic scheme of the system of complex analysis of milk lipids and milk products according to M.S. Umansky As a result of the above system of cognition research object, at the example of an idealized model of the most sophisticated biotechnological objectcheesemaking, we can formulate principles of Lipidomics of dairy industry: lipid complex and lipase of raw milk, analytics of lipids and lipase, enzymesbuilders, biogenic enzymes of bacterial cultures, lipolysis in the cheese during its production, storage and maturation. A single technique aimed to establish laws governing the presence of somatic cells in raw milk and active lipoprotein (which is not considered in industry) needs a separate independent study and practical implementation. Then the problem is in a logical sequence.

RESULTS AND DISCUSSION
Lipids (Greek Lipos -fat) are hydrophobic (insoluble in water but soluble in organic solvents) chemical compounds with different composition and structure [10]. In everyday practice we know animal fats, such as milk fat, vegetable oil and microbial oil (there are six product groups with more than 20 items). Among lipids (fats) of animal origin, and in particular in vegetable oils, a unique place is taken by the subject of this article -milk fat, the main indicator of the value of raw milk in Russia. The uniqueness of this bioenergy power given by nature at the disposal of "homo-sapies" can not be overestimated, and to understand it is very simple. Just have a portion of the natural (preferably fresh) and skim milk. Everyone will understand the difference! Milk fat generates a dairy product -its taste, aftertaste, color and even "childhood memories" about your nurse-mother, "fairy tales and songs" (Alexander Pushkin).
This systematic file represents identified and isolated milk lipids according to A. Tepel [3] as neutral fat (acyl glycerol), lipoids (fat-like compounds), isoprenoid lipids and substances related to lipids (Fig. 4).
The main component of the lipid complex of milkmilk fat (glycerides) -occupy more than 98%. The remaining 2% of the lipids (related substances) are phospholipids, glycolipids, sterols, vitamins, pigments; they are usually a part of the lipoprotein membranes of the fat globules -formalized milk fat. Milk fat is a complex hierarchical system which according to Mikhail Umansky includes all partially lipid compounds. It is shown in Fig. 5.    Fig. 1 and 2 is unique and is reduced in practice to milk fat cluster structures -globules. Biotechnology fat-containing milk products (cream, sour cream, and of course, butter) is connected with the fat globules. They also play a significant role in the formation of the entire range of protein-fat products -drinks, canned milk, condensed and dried milk, cottage cheese, all kinds of cheeses, etc. A common scientific base of the whole range of milk fat modification and possible derivatives is Lipidomics (science about lipid complex -milk fat). Its principles are formed for all assortment groups and individual milk and milk products. Lipidomics of cheese in complete technological cycle -cheese and whey is considered as a basic example (analog).
The general structural formula of milk fat triglycerides is shown below (R, R1 and R2 are hydrocarbon radicals of higher carboxylic acids).
Schematically fat globules of cow's milk at the present level according to A.Tepel [3] are shown in Fig. 6.
The starting components of the milk fat-structural elements of acyl glycerol -are fatty acids, characteristic of which is shown in Table 1.
The complexity of the structure of one of the groups of milk lipids is shown in Fig. 7.     It should be noted that based on the VFA of E.I. Melnikova, Professor of VGUIT [14] a new perspective is formed on the original component of milk whey -osmophoric compounds that deserve special consideration.
Milk fat has only about 5% in dry milk whey, but it plays an important role in industrial processes, even indirectly -phospholipids, lipoproteins and even fat sugar. Lipid complex is certainly separated from milk whey in the identified complex, separate fractions or together with other components. It should be noted that the component of milk fat globular membrane whey cream (MFGM), shown in Fig. 8 (on the left), attracted the attention of physicians. It is proved by the special report on the Sixth International Conference on milk whey (US, 2011) [15].
A special place in M.S. Umansky's researches [9] is given to lipolytic enzymes of raw milk, which classification is shown in Table 2.     (Table 4 second heat.  uidelines of li ky have no a low (Fig. 12

CONCLUSION
In general, a brief analysis of M.S. Umansky's contribution to the development of selective lipolysis in cheese in the author's edition "lipidology of cheese" underlines the greatness of his work and the possibility of forming Lipodimics of dairy industry. Moreover, it allows us to formulate the following conclusions.
(1) An independent scientific direction -lipidology of cheeses (initiators and leaders are Professor H.H. Dilanyan and L.A. Ostroumov) began to form in biotechnological research in milk production and dairy products in 70 years of the last century. Its development was promoted by the appearance of such research methods as gas-liquid and thin-layer chromatography, spectrophotometry, alkalimetry and others.
(2) Methodological framework for the implementation of the management ideas of lipolysis in cheese is created. It includes the development and modification of 17 original analytical methods of milk research, culture media, bacterial whey and starter cultures at different stages of cheese production. The main processes of lipolytic in milk are studied. It is shown that lipolysis scale depends on the presence of lipoprotein lipase in the milk and other lipolytic enzyme, which is the main producer of psychrophilic microorganisms. Lipid and fatty acid composition of different kinds of cheese is investigated. It is found that each of them is characterized by a certain content of ester compounds and their hydrolysis products. To assess the level of enzymatic catalysis of lipid fractions an integral indicator -ratio of fatty acid specificity -is proposed.
(3) We proved experimentally the ability of lactobacilli to produce enzymes of lipolytic orientation that hydrolyze triacylglycerols of milk fat forming diacylglycerols, monoacylglycerol and fatty acids. The degree of lipase and phospholipase activity of Lactococcus and Lactobacillus has interspecific and intraspecific differences. (4) We prove the existence of lipase and phospholipase activity in the majority of the representatives of propionic acid bacteria involved in the maturation of cheeses with a high temperature of second heating. The intensity of these symptoms at propionic acid bacteria is higher than at lactic acid bacteria. (5) The hypothesis is put forward that there is a connection between specificity and degree of lipolytic activity of lactic and propionic acid bacteria used as starter cultures, and the intensity and direction of enzymatic hydrolysis of lipids in ripening cheeses. Selection of microflora in such a way is an effective quality management tool and the length of cheese ripening. (6) The dependence of the formation processes of cheeses with a low temperature of second heating on the level of lipolytic activity of the lactic microflora starter cultures is set. (7) We determined lipolytic activity of 12 different kinds of milk-products and proposed the criterion system of evaluation on this indicator of their suitability in cheese making. (8) Original solution for the management process of cheese lipolysis is created, the novelty of them is protected by 15 copyright certificates for inventions (patents).
All above mentioned allows us to start the formation of Lipidomics postulates system form within Lactomics of dairy industry, starting with raw milk and its products in dairy products with a mandatory full and rational use of byproduct of raw milk-skim milk, milk whey and buttermilk. The legality of the implementation of systematic research is convincingly proved by Professor M.S. Umansky. The field of activity here is huge -all product groups and separate types of dairy products.