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Enzyme-Linked Immunosorbent Assay for the Determination of 2,4,6-Trinitrotoluene and Related Nitroaromatic Compounds

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Abstract

A sensitive enzyme-linked immunosorbent assay (ELISA) for the determination of the explosive 2,4,6-trinitrotoluene (TNT) and other nitroaromatic compounds was developed. As tracer, a trinitrophenyl-derivative conjugated to horseradish peroxidase was used. With this competitive assay, performed in polystyrene microtitre plates, TNT can be detected within the range of 0.02 μg/1 and 20μg/l. Cross-reactivities at the center points (50% values) of several nitroaromatic compounds were determined. Additionally, the cross-reactivities at different concentrations of the analytes were stated to yield a more realistic estimation of the interferences. Finally, the applicability of the immunoassay to the determination of TNT in water samples was assessed.

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Authors and Affiliations

  1. Institute of Hydrochemistry, Technical University of Munich, Marchioninistr. 17, D-8000, München 70, Germany

    Claudia Keuchel, Ludwig Weil & Reinhard Niessnert

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  1. Claudia Keuchel
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  2. Ludwig Weil
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  3. Reinhard Niessnert
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Keuchel, C., Weil, L. & Niessnert, R. Enzyme-Linked Immunosorbent Assay for the Determination of 2,4,6-Trinitrotoluene and Related Nitroaromatic Compounds. ANAL. SCI. 8, 9–12 (1992). https://doi.org/10.2116/analsci.8.9

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  • DOI: https://doi.org/10.2116/analsci.8.9

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