Abstract
A sensitive flow injection system for l-lactate with polarographic detection is described. The system mainly utilizes saturated dissolved oxygen in samples for enzymatic reactions to convert l-lactate and oxygen to pyruvate and hydrogen peroxide; and then, any excess amount of dissolved, electroreducible oxygen which is not consumed in the enzymatic reaction can be retarded by an ODS-coIumn to separate it from the pyruvate and hydrogen peroxide. After that, both the pyruvate and hydrogen peroxide are measured by the polarographic method using a static mercury drop electrode. The enzyme, lactate oxidase, was covalently immobilized on Chitopearl® composed of chitosan, and packed into a reactor. The detection limit under this system was 9.9× 10-6 M and the relative standard deviation at 2.0×10-4 M l-lactate was 2.8%.
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Hikima, S., Hasebe, K. & Kakizaki, T. Flow Injection Method for the Polarographic Determination of L-Lactate Using an Immobilized Enzyme Reactor. ANAL. SCI. 8, 165–168 (1992). https://doi.org/10.2116/analsci.8.165
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DOI: https://doi.org/10.2116/analsci.8.165