Analytical Sciences
Online ISSN : 1348-2246
Print ISSN : 0910-6340
ISSN-L : 0910-6340
HOMOGENEOUS CHEMILUMINESCENT IMMUNOASSAY BASED ON COMPLEMENT MEDIATED HEMOLYSIS
YOSHIRO TATSUSOICHIRO YAMAMURASUSUMU YOSHIKAWA
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1991 Volume 7 Issue Supple Pages 903-904

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Abstract

Human serum albumin (HSA) was determined by the homogeneous chemiluminescent immunoassay. The assay was performed by two methods, complement fixation method and competitive direct hemolysis method. After the complement mediated hemolysis of red blood cells, the chemiluminescence of luminol catalyzed by leaked hemoglobin was measured homogeneously. The sensitivities of HSA were 0.25 μg/ml and 0.1μg/ml, respectively but the latter method was convenient for the assay.

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© The Japan Society for Analytical Chemistry
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