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Rapid Enzyme-linked Immunosorbent Assays for Diagnosis of Diabetes in a Compact Disc-shaped Microfluidic Device

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Abstract

We have developed a compact disc (CD)-shaped microfluidic device for multiple, rapid enzyme-linked immunosorbent assays (ELISA). The device has a versatile design that can be adapted for the detection of various proteins by selecting the push-in-type reaction parts and appropriate reagents for each target. In this paper, we report the rapid quantification of insulin, adiponectin, and leptin, which can be used for the early diagnosis of diabetes, in human serum in only 16 min with our device.

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Acknowledgements

This work was financially supported by a grant from the Saitama Leading-edge Industry Design Project.

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Authors and Affiliations

  1. Biomedical Research Institute, National Institute of Advanced Industrial Science and Technology (AIST), 1-8-31 Midorigaoka, Ikeda, Osaka, 563-8577, Japan

    Shunsuke Furutani, Keiko Nishio, Nahoko Naruish, Yoko Akazawa-Ogawa, Yoshihisa Hagihara & Hidenori Nagai

  2. Advanced Photonics and Biosensing Open Innovation Laboratory (PhotoBIO-OIL), National Institute of AIST, 2-1 Yamadaoka, Suita, Osaka, 565-0871, Japan

    Shunsuke Furutani & Hidenori Nagai

  3. Health Research Institute, National Institute of AIST, 2217-14 Hayashi, Takamatsu, Kagawa, 761-0395, Japan

    Yasukazu Yoshida

Authors
  1. Shunsuke Furutani
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  2. Keiko Nishio
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  3. Nahoko Naruish
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  4. Yoko Akazawa-Ogawa
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  5. Yoshihisa Hagihara
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  6. Yasukazu Yoshida
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  7. Hidenori Nagai
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Correspondence to Hidenori Nagai.

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Furutani, S., Nishio, K., Naruish, N. et al. Rapid Enzyme-linked Immunosorbent Assays for Diagnosis of Diabetes in a Compact Disc-shaped Microfluidic Device. ANAL. SCI. 34, 379–382 (2018). https://doi.org/10.2116/analsci.34.379

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  • DOI: https://doi.org/10.2116/analsci.34.379

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