Abstract
In this study, amphiphilic diblock copolymers were designed and synthesized via the incorporation of reversible additionfragmentation chain transfer radical polymerization (RAFT) and a subsequent grafting technique. Subsequently, Hg2+- sensitive water-soluble fluorescent polymeric micelles (FNs) were prepared by a reprecipitation strategy. The spectroscopic characteristics demonstrate that the fluorescein isothiocyanate (FITC) was successfully linked into the polymer. Due to the promoted reaction of desulfurization cyclization by Hg2+, the fluorescence of fluorescein in FNs was obviously quenched. The as-prepared FNs showed admirable Hg2+-sensitivity (detection limit: 54 nM), excellent water-solubility and high selectivity. In addition, FNs were successfully used to determine Hg2+ in blood serum. We expected that the as-prepared FNs could perform potential applications in imaging, sensing, and bioanalytic chemistry.
Similar content being viewed by others
References
X. Zuo, H. Zhang, Q. Zhu, W. Wang, J. Feng, and X. Chen, Biosens. Bioelectron., 2016, 85, 464.
J. Hu, T. Wu, G. Zhang, and S. Liu, Macromolecules, 2012, 45, 3939.
N. Duan, B. Xu, S. Wu, and Z. Wang, Anal. Sci., 2016, 32, 431.
G. Feng, Y. Ding, Z. Gong, Y. Dai, and Q. Fei, Anal. Sci., 2013, 29, 735.
N. C. Gong, Y. L. Li, X. Jiang, X. F. Zheng, Y. Y. Wang, and S. Y. Huan, Anal. Sci., 2016, 32, 951.
M. Yuan, Y. Li, J. Li, C. Li, X. Liu, J. Lv, J. Xu, H. Liu, S. Wang, and D. Zhu, Org. Lett., 2007, 9, 2313.
Z. Zhang, D. Wu, X. Guo, X. Qian, Z. Lu, Q. Xu, Y. Yang, L. Duan, Y. He, and Z. Feng, Chem. Res. Toxicol., 2005, 18, 1814.
L. D. Hylander and M. E. Goodsite, Sci. Total Environ., 2006, 368, 352.
A. Renzoni, F. Zino, and E. Franchi, Environ. Res., 1998, 77, 68.
H. H. Harris, I. J. Pickering, and G. N. George, Science, 2003, 301, 1203.
D. W. Boening, Chemosphere, 2000, 40, 1335.
L. Cui, J. Wu, and H. Ju, ACS Appl. Mater. Interfaces, 2014, 6, 16210.
W. Xu, C. Ren, C. L. Teoh, J. Peng, S. H. Gadre, H. W. Rhee, C. Lee, and Y. T. Chang, Anal. Chem., 2014, 86, 8763.
Y. Gao, S. De Galan, A. De Brauwere, W. Baeyens, and M. Leermakers, Talanta, 2010, 82, 1919.
N. Bloom and W. F. Fitzgerald, Anal. Chim. Acta, 1988, 208, 151.
W. F. Fitzgerald and G. A. Gill, Anal. Chem., 1979, 51, 1714.
G. Pezzatinia and F. Pergola, J. Macromol. Sci. A, 1986, 19, 89.
F. Ma, M. Sun, K. Zhang, and S. Wang, Sens. Actuators, B, 2015, 209, 377.
X. Dai, Z.-Y. Wang, Z.-F. Du, J.-Y. Miao, and B.-X. Zhao, Sens. Actuators, B, 2016, 232, 369.
J. Chen, Y. Li, K. Lv, W. Zhong, H. Wang, Z. Wu, P. Yi, and J. Jiang, Sens. Actuators, B, 2016, 224, 298.
P. Zhang, J. Li, B. Li, J. Xu, F. Zeng, J. Lv, and S. Wu, Chem. Commun., 2015, 51, 4414.
Y. Liu, M. Liao, X. He, X. Liu, X. Kou, and D. Xiao, Anal. Sci., 2015, 31, 971.
Y. Wang, C. Dai, and X. P. Yan, Chem. Commun., 2014, 50, 14341.
C. Li, Y. Zhou, Y. Li, C. Zou, and X. Kong, Anal. Sci., 2013, 29, 899.
X. Guan, H. Fan, Y. Zhang, D. Zhang, T. Jia, S. Lai, and Z. Lei, Anal. Sci., 2016, 32, 161.
H. Mi, M. Guan, H. Shan, Q. Fei, Y. Huan, Z. Zhang, and G. Feng, Anal. Sci., 2016, 32, 1039.
J. Liu, Z. Xu, L. Xu, Z. Bian, G. Sang, and B. Zhu, Anal. Sci., 2016, 32, 361.
B. Ma, F. Zeng, F. Zheng, and S. Wu, Chem.-Eur J., 2011, 17, 14844.
L. Mujawar, A. A. Felemban, and M. S. El-Shahawi, Anal. Sci., 2016, 32, 491.
H. Zheng, Z. H. Qian, L. Xu, F. F. Yuan, L. D. Lan, and J. G. Xu, Org. Lett., 2006, 8, 859.
J. Chen, Y. Li, W. Zhong, H. Wang, P. Zhang, and J. Jiang, Anal. Methods, 2016, 8, 1964.
H. Zhou, W. Tian, M. Jiang, P. Li, S. Zeng, W. Chen, and A. Ma, Anal. Sci., 2015, 31, 1285.
E. M. Nolan and S. J. Lippard, Chem. Rev., 2008, 108, 3443.
P. Zhang, J. Chen, F. Huang, Z. Zeng, J. Hu, P. Yi, F. Zeng, and S. Wu, Polym. Chem., 2013, 4, 2325.
C. Ma, F. Zeng, L. F. Huang, and S. Wu, J. Phys. Chem. B, 2011, 115, 874.
J. Hu, L. Dai, and S. Liu, Macromolecules, 2011, 44, 4699.
Z. Wang, Z. K. Yang, T. Gao, J. W. He, L. J. Gong, Y. B. Lu, Y. Q. Xiong, and W. J. Xu, Anal. Methods, 2015, 7, 2738.
F. J. Orriach-Fernández, A. Medina-Castillo, J. F. Fernández-Sánchez, A. Muñoz de la Peña, and A. Fernández-Gutiérrez, Anal. Methods, 2013, 5, 6642.
J. Chen, W. B. Zhong, Y. Tang, Z. Wu, Y. Li, P. G. Yi, and J. H. Jiang, Macromolecules, 2015, 48, 3500.
D. Wu, W. Huang, Z. Lin, C. Duan, C. He, S. Wu, and D. Wang, Inorg. Chem., 2008, 47, 7190.
G. Aragay, J. Pons, and A. Merkoci, Chem. Rev., 2011, 111, 3433.
B. Liu and H. Tian, Chem. Commun., 2005, 3156.
R. A. Batey and D. A. Powell, Org. Lett., 2000, 20, 3237.
C. Wang, D. Zhang, X. Huang, P. Ding, Z. Wang, Y. Zhao, and Y. Ye, Sens. Actuators, B, 2014, 198, 33.
J. Manimala and E. Anslyn, Eur. J. Org. Chem., 2002, 3909.
K. S. Kim and L. Qian, Tetrahedron Lett., 1993, 34, 7677.
D. Boeglin, S. Cantel, A. Heitz, J. Martinez, and J. A. Fehrentz, Org. Lett., 2003, 5, 4465.
S. Dahmen and S. Bräse, Org. Lett., 2000, 2, 3563.
S. Cunha, M. S. Costa, H. B. Napolitano, C. Lariucci, and I. Vencato, Tetrahedron, 2001, 57, 1671.
S. J. Wu, I. C. Hwang, and K. S. Kim, Org. Lett., 2007, 9, 907.
M. H. Lee, S. W. Lee, S. H. Kim, C. Kang, and J. S. Kim, Org. Lett., 2009, 11, 2101.
Acknowledgments
This work was supported by NSFC (Project No. 51373002, 51603067), Scientific Research Foundation for the Returned Overseas Chinese Scholars, Project funded by China Postdoctoral Science Foundation (Project No. 2014M550418, 2015T80867), Open Project Program of State Key Laboratory of Chemo/Biosensing and Chemometrics (Project No. 2013008) and Open Project Program of Key Laboratory for High Performance and Functional Polymer Materials of Guangdong Province (South China University of Technology) (Project No. 20160005).
Author information
Authors and Affiliations
Corresponding authors
Electronic supplementary material
Rights and permissions
About this article
Cite this article
Wang, H., Chen, J., Hong, Y. et al. Fabrication of Water-soluble Fluorescent Polymeric Micelles for Selective Detection of Hg2+ in Blood Serum. ANAL. SCI. 33, 591–598 (2017). https://doi.org/10.2116/analsci.33.591
Received:
Accepted:
Published:
Issue Date:
DOI: https://doi.org/10.2116/analsci.33.591