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Structural Stability of Light-harvesting Protein LH2 Adsorbed on Mesoporous Silica Supports

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Abstract

In the present study, we examined the reversible thermal deformation of the membrane protein light-harvesting complex LH2 adsorbed on mesoporous silica (MPS) supports. The LH2 complex from Thermochromatium tepidum cells was conjugated to MPS supports with a series of pore diameter (2.4 to 10.6 nm), and absorption spectra of the resulting LH2/MPS conjugates were observed over a temperature range of 273 – 313 K in order to examine the structure of the LH2 adsorbed on the MPS support. The experimental results confirmed that a slight ellipsoidal deformation of LH2 was induced by adsorption on the MPS supports. On the other hand, the structural stability of LH2 was not perturbed by the adsorption. Since the pore diameter of MPS support did not influence the structural stability of LH2, it could be considered that the spatial confinement of LH2 in size-matches pore did not improve the structural stability of LH2.

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Authors and Affiliations

  1. College of Science, Ibaraki University, 2-1-1 Bunkyo, 310-8512, Mito, Ibaraki, Japan

    Yuuta Shibuya & Akira Yamaguchi

  2. Research Institute for Chemical Process Technology, National Institute of Advanced Industrial Science and Technology (AIST), 4-2-1 Nigatake, 983-8551, Miyagino, Sendai, Japan

    Tetsuji Itoh & Shun-ichi Matsuura

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  1. Yuuta Shibuya
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  2. Tetsuji Itoh
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  3. Shun-ichi Matsuura
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  4. Akira Yamaguchi
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Correspondence to Akira Yamaguchi.

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Shibuya, Y., Itoh, T., Matsuura, Si. et al. Structural Stability of Light-harvesting Protein LH2 Adsorbed on Mesoporous Silica Supports. ANAL. SCI. 31, 1069–1074 (2015). https://doi.org/10.2116/analsci.31.1069

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  • DOI: https://doi.org/10.2116/analsci.31.1069

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