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Optical Characterization of the Antigen-Antibody Thin Layer Using the Whispering Gallery Mode

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Abstract

We immobilized an antibody (anti-β-Galactosidase) on a polystyrene microsphere by using a covalent bond, and observed the resonance peaks in the scattered light intensity spectra related to the whispering gallery mode (WGM) excitation of the microsphere. The amount and the optical parameters, i.e., thickness and refractive index, of anti-β-Galactosidase on the sphere surface were evaluated based on an absorbance measurement and a resonance peak shift measurement, respectively. Moreover, we measured the variation of the WGM spectra depending on the concentration of the enzyme solution (β-Galactosidase), which allowed us to optically evaluate the thickness and the refractive index of the antigen-antibody layer from the shift of the WGM spectra peak.

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Authors and Affiliations

  1. Industrial Technology Center of Nagasaki, 2-1303-8 Ikeda, 856-0026, Omura, Nagasaki, Japan

    Takeshi Tajiri & Shuzo Matsumoto

  2. Department of Optical Science and Technology, Faculty of Engineering, The University of Tokushima, 2-1 Minamijosanjima, 770-8506, Tokushima, Japan

    Takeshi Tajiri, Toshihiro Okamoto & Masanobu Haraguchi

  3. Department of Applied Chemistry, Graduate School of Engineering, Kyushu University, 744 Moto-oka, 819-0395, Nishi, Fukuoka, Japan

    Toshihiko Imato

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  1. Takeshi Tajiri
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  2. Shuzo Matsumoto
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  3. Toshihiko Imato
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  4. Toshihiro Okamoto
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  5. Masanobu Haraguchi
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Correspondence to Takeshi Tajiri.

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Tajiri, T., Matsumoto, S., Imato, T. et al. Optical Characterization of the Antigen-Antibody Thin Layer Using the Whispering Gallery Mode. ANAL. SCI. 30, 799–804 (2014). https://doi.org/10.2116/analsci.30.799

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  • DOI: https://doi.org/10.2116/analsci.30.799

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