Skip to main content
Log in

Promotion and Suppression Effects of Cationic Polymer ε-Poly-L-lysine on the Glucose Oxidase Reaction with Ferrocene Derivatives as Oxidants with Different Charges

  • Published:
Analytical Sciences Aims and scope Submit manuscript

Abstract

The effects of the cationic polymer ε-poly-L-lysine (εPL) on the enzymatic reaction rate of glucose oxidase (GOD) with ferrocene derivatives having different ionic charges have been investigated by measuring the ferrocene derivative-mediated catalytic current of oxidation by GOD. When negatively charged ferrocenes were used, the bioelectrocatalytic current, which is related to the enzymatic reaction rate, was increased by the addition of εPL. On the other hand, the reaction rates with positively charged ferrocenes were decreased by εPL. These promotion and suppression effects of εPL were remarkable at a certain pH range, where εPL and GOD were charged positively and negatively, respectively. Within this range, the polycationic εPL would be adsorbed onto the GOD surface to enhance the electrostatic interactions of the enzyme with negatively charged substrates, and repulsion with positively charged ones. These findings should be important for practical applications of enzymes.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  1. R. Wilson and A. P. F. Turner, Biosens. Bioelectron., 1992, 7, 165.

    Article  CAS  Google Scholar 

  2. E. Liaudet, F. Battaglini, and E. J. Calvo, J. Electroanal. Chem., 1990, 293, 55.

    Article  CAS  Google Scholar 

  3. D. M. Fraser, S. M. Zakeeruddin, and M. Gratzel, J. Electroanal. Chem., 1993, 359, 125.

    Article  CAS  Google Scholar 

  4. S. Ikeda, T. Yoshioka, and S. Nankai, Electrochemistry, 1995, 12, 1145.

    Google Scholar 

  5. A. E. G. Cass, G. Davis, G. D. Francis, H. A. O. Hill, W. J. Aston, I. J. Higgins, E. V. Plotkin, L. D. L. Scott, and A. P. F. Turner, Anal. Chem., 1984, 56, 667.

    Article  CAS  PubMed  Google Scholar 

  6. J. J. Kulys and N. K. Cenas, Biochim. Biophys. Acta, 1983, 744, 57.

    Article  CAS  Google Scholar 

  7. K. Uematsu, M. Yamasaki, T. Hibi, and H. Katano, Anal. Sci., 2012, 28, 657.

    Article  CAS  PubMed  Google Scholar 

  8. H. Katano, K. Uematsu, C. Maruyama, and Y. Hamano, Anal. Sci., 2014, 30, 17.

    Article  CAS  PubMed  Google Scholar 

  9. S. Shima and H. Sakai, Agric. Biol. Chem., 1981, 45, 2503.

    CAS  Google Scholar 

  10. H. Katano, T. Yoneoka, N. Kito, C. Maruyama, and Y. Hamano, Anal. Sci., 2012, 28, 1153.

    Article  CAS  PubMed  Google Scholar 

  11. H. Katano, Y. Sugimoto, K. Uematsu, and T. Hibi, Anal. Sci., 2011, 27, 979.

    Article  CAS  PubMed  Google Scholar 

  12. T. Ohgaru, H. Tatsumi, K. Kano, and T. Ikeda, J. Electroanal. Chem., 2001, 496, 37.

    Article  CAS  Google Scholar 

  13. R. Matsumoto, K. Kano, and T. Ikeda, J. Electroanal. Chem., 2002, 535, 37.

    Article  CAS  Google Scholar 

  14. H. Katano, H. Tatsumi, T. Hibi, T. Ikeda, and T. Tsukatani, Anal. Sci., 2008, 24, 1421.

    Article  CAS  PubMed  Google Scholar 

  15. H. Katano, K. Uematsu, T. Hibi, T. Ikeda, and T. Tsukatani, Anal. Sci., 2009, 25, 1077.

    Article  CAS  PubMed  Google Scholar 

  16. T. Ikeda, K. Uematsu, H. Ma, H. Katano, and T. Hibi, Anal. Sci., 2009, 25, 1283.

    Article  CAS  PubMed  Google Scholar 

  17. K. Uematsu and H. Katano, Anal. Sci., 2013, 29, 25.

    Article  CAS  PubMed  Google Scholar 

  18. Biochemical Data Book, vol. 1 (in Japanese)”, ed. Japan Biochemical Society, 1979, Tokyo Kagaku Dojin, Tokyo.

  19. B. E. P. Swoboda and V. Massay, J. Biol. Chem., 1965, 240, 2209.

    Article  CAS  PubMed  Google Scholar 

  20. Y. Ogino, K. Takagi, K. Kano, and T. Ikeda, J. Electroanal. Chem., 1995, 396, 517.

    Article  Google Scholar 

  21. H. Katano, H. Tatsumi, T. Hibi, T. Ikeda, and T. Tsukatani, Anal. Sci., 2008, 24, 1415.

    Article  CAS  PubMed  Google Scholar 

Download references

Author information

Authors and Affiliations

Authors

Corresponding author

Correspondence to Kohei Uematsu.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Uematsu, K., Minami, Y., Taira, S. et al. Promotion and Suppression Effects of Cationic Polymer ε-Poly-L-lysine on the Glucose Oxidase Reaction with Ferrocene Derivatives as Oxidants with Different Charges. ANAL. SCI. 30, 299–303 (2014). https://doi.org/10.2116/analsci.30.299

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.2116/analsci.30.299

Keywords

Navigation