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Electroanalysis and Simultaneous Determination of 6-Thioguanine in the Presence of Uric Acid and Folic Acid Using a Modified Carbon Nanotube Paste Electrode

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Abstract

The present work describes the preparation and characterization of a carbon nanotube paste electrode modified with 2,7-bis(ferrocenyl ethyl)fluoren-9-one (2,7-BF). This electrode showed an efficient catalytic activity for the electro-oxidation of 6-thioguanine (6-TG), which leads to lowering 6-TG overpotential by more than 610 mV. Also, the values of catalytic rate constant (k = 2.7 x 103 mol-1 L s-1), and diffusion coefficient (D = 2.7 x 10-5 cm2 s) were calculated. In 0.1 M phosphate buffer solution of pH 7.0, the oxidation current increased linearly with two concentration intervals of 6-TG, one is 0.06 to 10.0 µmol L-1 and the other is 10.0 to 160.0 µmol L-1. The detection limit (3σ) obtained by differential pulse voltammetry (DPV) was 22.0 nmol L-1. DPV was used for simultaneous determination of 6-TG, uric acid (UA) and folic acid (FA) at the modified electrode, and for quantification of 6-TG, UA and FA in some real samples by the standard addition method.

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Authors and Affiliations

  1. Environment Department, Research Institute of Environmental Sciences, International Center for Science, High Technology and Environmental Sciences, Kerman, Iran

    Hadi Beitollahi

  2. Electroanalytical Chemistry Research Laboratory, Department of Analytical Chemistry, Faculty of Chemistry, University of Mazandaran, Babolsar, Iran

    Jahan-Bakhsh Raoof

  3. Department of Organic Chemistry, Faculty of Chemistry, University of Mazandaran, Babolsar, Iran

    Rahman Hosseinzadeh

Authors
  1. Hadi Beitollahi
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  2. Jahan-Bakhsh Raoof
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  3. Rahman Hosseinzadeh
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Correspondence to Hadi Beitollahi.

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Beitollahi, H., Raoof, JB. & Hosseinzadeh, R. Electroanalysis and Simultaneous Determination of 6-Thioguanine in the Presence of Uric Acid and Folic Acid Using a Modified Carbon Nanotube Paste Electrode. ANAL. SCI. 27, 991–997 (2011). https://doi.org/10.2116/analsci.27.991

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  • DOI: https://doi.org/10.2116/analsci.27.991

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