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Enzyme-Release Capillary as a Facile Enzymatic Biosensing Part for a Capillary-Assembled Microchip

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Abstract

A simple capillary enzymatic biosensor was developed. This was prepared by simply coating a dissolvable membrane containing enzyme/s on the inner wall of a square glass capillary. An easy measurement was carried out by capillary force sample introduction with concurrent enzyme release and a reaction with a certain substrate. Enzyme-release capillary (ERC) biosensors showed long-term storage stability of at least two weeks for a β-galactoside derivative and glucose. Moreover, this could be integrated on a capillary-assembled microchip (CAs-CHIP) to broaden its multiple analyte sensing potential for clinical diagnostic applications.

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Authors and Affiliations

  1. Department of Applied Chemistry, Graduate School of Engineering, Osaka Prefecture University, 1-1 Gakuen, 599-8531, Naka, Sakai, Osaka, Japan

    Terence G. Henares, Fumito Okubo, Toshio Yao & Hideaki Hisamoto

  2. Graduate School of Material Science, University of Hyogo, 3-2-1 Kouto, 678-1297, Kamigori, Ako, Hyogo, Japan

    Eiji Maekawa & Fumio Mizutani

  3. Metaboscreen Co. Ltd., 34-1-412 Terakubo, 231-0855, Naka, Yokohama, Japan

    Ryuichi Sekizawa

Authors
  1. Terence G. Henares
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  2. Eiji Maekawa
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  3. Fumito Okubo
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  4. Fumio Mizutani
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  5. Toshio Yao
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  6. Ryuichi Sekizawa
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  7. Hideaki Hisamoto
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Corresponding author

Correspondence to Hideaki Hisamoto.

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Henares, T.G., Maekawa, E., Okubo, F. et al. Enzyme-Release Capillary as a Facile Enzymatic Biosensing Part for a Capillary-Assembled Microchip. ANAL. SCI. 25, 1025–1028 (2009). https://doi.org/10.2116/analsci.25.1025

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  • DOI: https://doi.org/10.2116/analsci.25.1025

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