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Batch-Type Detection Cell Using a Peroxyoxalate Chemiluminescence System for Capillary Electrophoresis

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Abstract

A batch-type chemiluminescence detection cell using a peroxyoxalate system was easily combined with capillary elec-trophoresis equipment; the device was operated without any tedious procedures. Peroxyoxalate reagents and hydrogen peroxide were examined in detail concerning their usage and concentration with the cell. Fluorescence-labeled a -amino acids showed the detection limits of 10–100 nM order (S/N=3), which were approximately 2 or 3 orders of magnitude lower than those obtained with the absorbance. A mixture sample of the labeled a -amino acids was successfully separated and detected.

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Authors and Affiliations

  1. Department of Chemical Engineering and Materials Science, Faculty of Engineering, Doshisha University, Kyotanabe, Kyoto, 610-0321, Japan

    Kazuhiko Tsukagoshi, Miwa Otsuka, Masahiko Hashimoto, Riichiro Nakajima & Kazuo Kondo

Authors
  1. Kazuhiko Tsukagoshi
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  2. Miwa Otsuka
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  3. Masahiko Hashimoto
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  4. Riichiro Nakajima
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  5. Kazuo Kondo
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Tsukagoshi, K., Otsuka, M., Hashimoto, M. et al. Batch-Type Detection Cell Using a Peroxyoxalate Chemiluminescence System for Capillary Electrophoresis. ANAL. SCI. 15, 1257–1260 (1999). https://doi.org/10.2116/analsci.15.1257

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  • DOI: https://doi.org/10.2116/analsci.15.1257

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