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Determination of Some Metal-EDTA Complexes by Capillary Type Isotachophoresis

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Abstract

To establish the optimum conditions for the simultaneous determination of EDTA, Mg(II)-EDTA and Ca(II)-EDTA complexes by capillary type isotachophoresis, effects of the length of the precolumn and the main column, kinds of viscous agents, counter ions and terminating electrolytes, pH of leading electrolyte and the amount of sample injected into the analyzer were studied. The isotachophoretic separation was performed with analyzer equipped with a potential gradient detector. The migration current was initially set at 200 μA and then changed to 100 μA. The best result was obtained with a main column (40 cm in length and 0.5 mm in inner diameter) connected to a precolumn (4 cm in length and 1.0 mm in inner diameter) using a leading electrolyte containing 0.01 M hydrochloric acid and 0.5% methyl cellulose buffered at pH 8.5 with tris(hydroxymethyl)aminomethane, and a terminating electrolyte containing 0.01 M hexanoic acid. Under these conditions, Cu(II)-EDTA and Mn(II)-EDTA were also determined successfully. The error in the determination of the mixtures of these EDTA complexes was less than ±14%.

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Authors and Affiliations

  1. Kobe University of Mercantile Marine, Fukae, Higashinada-ku, Kobe, 658, Japan

    Keiichi Fukushi

  2. Government Industrial Research Institute, Osaka, Midorigaoka, Ikeda, Osaka, 563, Japan

    Kazuo Hiiro

Authors
  1. Keiichi Fukushi
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  2. Kazuo Hiiro
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Fukushi, K., Hiiro, K. Determination of Some Metal-EDTA Complexes by Capillary Type Isotachophoresis. ANAL. SCI. 1, 345–347 (1985). https://doi.org/10.2116/analsci.1.345

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  • DOI: https://doi.org/10.2116/analsci.1.345

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