Abstract
The simultaneous determination of (R)- and (S)-penbutolols in plasma by high-performance liquid chromatography (HPLC) with fluorescence detection is described. The Penbutolol enantiomers were derivatized with a chiral axis reagent, (aS)-2′-methoxy-1,1′-binaphthalene-2-carbonyl cyanide, and the diastereomeric esters formed were resolved by HPLC on a normal-phase column employing 0.005% triethylamine in hexane/ethyl acetate (6:1, v/v) as a mobile phase. The combined use of a Sep-pak C18 cartridge and an ion-exchange gel, carboxymethyl Sephadex LH-20, was effective for efficient cleaning of Penbutolol in plasma. The enantiomeric drugs spiked to the plasma were recovered at a rate of over 96% with satisfactory reproducibility. The quantitation limit of each enantiomer was 500 pg/ ml. The plasma-level profile of Penbutolol enantiomers in a dog administered with the racemate was investigated by the newly developed method.
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Goto, J., Shao, G., Ito, M. et al. High-Performance Liquid Chromatographic Determination of Penbutolol Enantiomers in Plasma with Fluorescence Detection. ANAL. SCI. 7, 723–726 (1991). https://doi.org/10.2116/analsci.7.723
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DOI: https://doi.org/10.2116/analsci.7.723