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A Graphene Quantum Dots-Enzyme Hybrid System for the Fluorescence Assay of Alkaline Phosphatase Activity and Inhibitor Screening

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Abstract

A graphene quantum dots (GQDs) and horse radish peroxidase (HRP) hybrid system was designed for the sensing of alkaline phosphatase (ALP) activity and inhibitor screening. We found that the photoluminescence (PL) intensity of GQDs could be quenched efficiently in the presence of phenol, H2O2 and HRP. Moreover, ALP could hydrolyze disodium phenyl phosphate (DPP) to produce phenol, and also could result in the photoluminescence quenching of GQDs. The decrease in the PL intensity was linear to the activity of ALP in the concentration range of 0.02–0.8 U/L, with a detection limit of 0.008 U/L. The proposed GQDs/HRP hybrid system was successfully applied to ALP determination in human serum samples. The inhibition study was further analyzed, and Na3VO4 (as an ALP inhibitor) showed a clear inhibition effect. The results suggest that the GQDs/HRP hybrid system has good potential applications for the assay of ALP activity and inhibitors screening in related biochemical fields.

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Acknowledgments

This work was financially supported by the National Natural Science Foundation of China (21405056, 31772058 and 21405151), the Science and Technology Development Project of Jilin province, China (20160520127JH and 20150520134JH), the China Postdoctoral Science Foundation (2016T90260 and 2013M541309), and Fundamental Research Funds for the Central Universities.

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Authors and Affiliations

  1. College of Food Science and Engineering, Jilin University, Changchun, 130025, China

    Hui Huang, Bingdi Wang, Meng Chen & Wenjing Zhang

  2. Key Laboratory of Bionic Engineering (Ministry of Education), College of Biological and Agricultural Engineering, Jilin University, Changchun, 130022, China

    Hui Huang, Bingdi Wang & Zhenning Liu

  3. State Key Laboratory of Electroanalytical Chemistry’, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun, 130022, China

    Yongxin Li

Authors
  1. Hui Huang
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  2. Bingdi Wang
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  3. Meng Chen
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  4. Wenjing Zhang
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  5. Yongxin Li
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Correspondence to Hui Huang, Yongxin Li or Zhenning Liu.

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Huang, H., Wang, B., Chen, M. et al. A Graphene Quantum Dots-Enzyme Hybrid System for the Fluorescence Assay of Alkaline Phosphatase Activity and Inhibitor Screening. ANAL. SCI. 34, 131–136 (2018). https://doi.org/10.2116/analsci.34.131

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