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Aptamer-based Colorimetrie Biosensing of Ochratoxin A in Fortified White Grape Wine Sample Using Unmodified Gold Nanopartieles

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Abstract

In this study, a simple and sensitive aptamer-based colorimetric method for the detection of Ochratoxin A by using gold nanoparticles (AuNPs) has been developed. In this assay, unmodified gold nanoparticles (AuNPs) were used as probes with a 36-mer aptamer as recognition element. In the absence of ochratoxin A, free aptamer could be adsorbed onto the surface of AuNPs and protect AuNPs from aggregation even with high concentrations of salt. The salt-induced aggregation of AuNPs was caused by the specific recognition of aptamers with OTA. Under optimum conditions, calibration modeling showed that the analytical linear range covered from 32 to 1024 ng/mL and the detection limit of 20 ng/mL was realized successfully. This proposed colorimetric bio-assay also showed high selectivity over other antibody based methods. Meanwhile, this strategy was further used to determine the concentrations of ochratoxin A in white wine sample with satisfying recovery rates.

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References

  1. A. Rhouati, C. Yang, A. Hayat, and J. L. Marty, Toxins, 2013, 5, 1988.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  2. L. Yang, Z. Ying, R. Li, C. Lin, L. Guo, B. Qiu, Z. Lin, and G. Chen, Biosens. Bioelectron., 2015, 70, 268.

    Article  CAS  PubMed  Google Scholar 

  3. W. Ma, H. Yin, L. Xu, Z. Xu, H. Kuang, L. Wang, and C. Xu, Biosens. Bioelectron., 2013, 42, 545.

    Article  CAS  PubMed  Google Scholar 

  4. J. Chen, Z. Xi, S. Cai, D. Wu, C. Mei, S. Wang, and J. Zhang, Biosens. Bioelectron., 2014, 57C, 226.

    Article  Google Scholar 

  5. H. P. van Egmond, R. C. Schothorst, M. A. Jonker, and Anal. Bioanal. Chem., 2007, 389, 147.

    Article  CAS  PubMed  Google Scholar 

  6. R. Liu, Y. Huang, Y. Ma, S. Jia, M. Gao, J. Li, and H. Zhang, ACS Photonics, 2015, 7, 6982.

    CAS  Google Scholar 

  7. X. Yang, W. Kong, Y. Hu, M. Yang, L. Huang, M. Zhao, and Z. Ouyang, J. Sep. Sci., 2014, 37, 853.

    Article  CAS  PubMed  Google Scholar 

  8. Y. Zheng, Y. Wang, and X. Yang, Sens Actuators, B, 2011, 156, 95.

    Article  CAS  Google Scholar 

  9. J. Chen, Z. Li, J. Ge, R. Yang, L. Zhang, L. B. Qu, H. Q. Zhang, and L. Zhang, Talanta, 2015, 139, 226.

    Article  CAS  PubMed  Google Scholar 

  10. L. Barthelmebs, A. Hayat, A. W. Limiadi, J. L. Marty, and T. Noguer, Sens, Actuators, B, 2011, 156, 932.

    Article  CAS  Google Scholar 

  11. J. Zhang, X. Zhang, G. Yang, J. Chen, and S. Wang, Biosens. Bioelectron., 2013, 41, 704.

    Article  PubMed  Google Scholar 

  12. A. Rhouati, C. Yang, A. Hayat, and J. L. Marty, Toxins, 2013, 5, 1988.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  13. Y. Luo, J. Xu, Y. Li, H. Gao, J. Guo, F. Shen, and C. Sun, Food Control, 2015, 54, 7.

    Article  CAS  Google Scholar 

  14. C. Luo, RSC Adv., 2014, 5, 10994.

    Article  Google Scholar 

  15. M. R. Hormozi-Nezhad and S. Abbasi-Moayed, Talanta, 2014, 129, 227.

    Article  CAS  PubMed  Google Scholar 

  16. Z. Chen, Y. Hu, Q. Yang, C. Wan, Y. Tan, and H. Ma, Sens. Actuators, B, 2015, 207, 277.

    Article  CAS  Google Scholar 

  17. C. Jing, Z. Li, G. Jia, Y. Ran, Z. Lin, L. B. Qu, and L. Zhang, Talanta, 2015, 139, 226.

    Article  Google Scholar 

  18. S. Cai, L. Xin, C. Lau, and J. Lu, Analyst, 2010, 135, 615.

    Article  CAS  PubMed  Google Scholar 

  19. C. Yang, Y. Wang, J.-L. Marty, and X. Yang, Biosens. Bioelectron., 2010, 26, 2724.

    Article  PubMed  Google Scholar 

  20. D. Zhao, C. Chen, L. Lu, F. Yang, and X. Yang, Sens. Actuators, B, 2015, 215, 437.

    Article  CAS  Google Scholar 

  21. M. R. Hormozi-Nezhad and S. Abbasi-Moayed, Talanta, 2014, 129, 227.

    Article  CAS  PubMed  Google Scholar 

  22. J. Hu, P. Ni, H. Dai, Y. Sun, Y. Wang, S. Jiang, and Z. Li, Analyst, 2015, 140, 3581.

    Article  CAS  PubMed  Google Scholar 

  23. S. Wang, S. Gao, S. Sun, Y. Yang, Y. Zhang, J. Liu, and Y. Dong, RSC Adv., 2016, 6, 45651.

    Google Scholar 

  24. Y. S. Kim, J. H. Kim, I. A. Kim, S. J. Lee, J. Jurng, and M. B. Gu, Biosens. Bioelectron., 2010, 26, 1644.

    Article  CAS  PubMed  Google Scholar 

  25. C. Wang, J. Qian, K. Wang, X. Yang, Q. Liu, and N. Hao, Biosens. Bioelectron., 2016, 77, 1183.

    Article  CAS  PubMed  Google Scholar 

  26. A. T. A. Gomes, D. Paredes, and F. Valentin, Procedia Computer Science, 2011, 4, 688.

    Article  Google Scholar 

Download references

Acknowledgments

This research was supported by Beijing “Thirteen-Five” food safety research on the key technology and realization of path (Grant No. Z141108001314016) together with the Key Program of a Synchronized Detection Research and Risk Assessment for Radionuclides in Foods of Beijing Municipal Science & Technology Commission (D161100002116002).

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Author notes

  1. X. Y. and S. W. contributed equally to this work.

Authors and Affiliations

  1. Beijing Key Laboratory of Bioprocess, College of Life Science and Technology, Beijing University of Chemical Technology, Beijing, 100029, P. R. China

    Xueting Yin, Sai Wang, Xiaoyun Liu, Chenmeng He, Yali Tang, Qimeng Li, Jiahui Liu, Haijia Su, Tianwei Tan & Yiyang Dong

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  1. Xueting Yin
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  2. Sai Wang
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  3. Xiaoyun Liu
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  4. Chenmeng He
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  7. Jiahui Liu
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  9. Tianwei Tan
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Correspondence to Yiyang Dong.

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Yin, X., Wang, S., Liu, X. et al. Aptamer-based Colorimetrie Biosensing of Ochratoxin A in Fortified White Grape Wine Sample Using Unmodified Gold Nanopartieles. ANAL. SCI. 33, 659–664 (2017). https://doi.org/10.2116/analsci.33.659

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  • DOI: https://doi.org/10.2116/analsci.33.659

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