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A Label-free and Turn-on Fluorescence Strategy for Kanamycin Detection Based on the NMM/G-quadruplex Structure

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Abstract

In the present work, a simple design of a “label-free” fluorescence method for kanamycin was developed and explored based on a specific DNA aptamer as a recognizer and a NMM/G-quadruplex DNA system as a reporter. The titration experiment showed a linear detection range of 0.5 to 100 nM with a low detection limit of 0.5 nM. It also showed excellent selectivity in a selectivity experiment. It was then successfully employed to detect kanamycin in milk with a excellent reliability.

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Acknowledgments

The research was supported by the National Natural Science Foundation of China (31601536), the Long-term Development Plan UHK, and the Yangtze Fund for Youth Teams of Science and Technology Innovation.

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Authors and Affiliations

  1. Jingchu Food Research and Development Center, College of Life Science, Yangtze University, Jingzhou, Hubei, 434025, China

    Hualin Yang, Dongxiao Su, Yun Wang, Li Li & Xingping Zhang

  2. Department of Chemistry, Faculty of Science, University of Hradec Kralove, Hradec Kralove, Czech Republic

    Qinghua Wu

Authors
  1. Hualin Yang
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  2. Qinghua Wu
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  3. Dongxiao Su
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  4. Yun Wang
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  5. Li Li
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  6. Xingping Zhang
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Correspondence to Xingping Zhang.

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Yang, H., Wu, Q., Su, D. et al. A Label-free and Turn-on Fluorescence Strategy for Kanamycin Detection Based on the NMM/G-quadruplex Structure. ANAL. SCI. 33, 133–135 (2017). https://doi.org/10.2116/analsci.33.133

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  • DOI: https://doi.org/10.2116/analsci.33.133

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