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A Portable Liquid Chromatograph with a Battery-operated Compact Electroosmotic Pump and a Microfluidic Chip Device with a Reversed Phase Packed Column

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Abstract

A compact and lightweight liquid chromatography system is presented with overall dimensions of 26 cm width × 18 cm length × 21 cm height and weight of 2 kg. This system comprises a battery-operated compact electroosmotic pump, a manual injector, a microfluidic chip device containing a packed column and an electrochemical detector, and a USB bus-powered potentiostat. The pumping system was designed for microfluidic-based reversed-phase liquid chromatography in which an electroosmotically generated water stream pushes the mobile phase via a diaphragm for the output. The flow rate ranged from 0 to 10 μL/min and had a high degree of precision. The pumping system operated continuously for over 24 h with dry batteries. The column formed in the microfluidic device was packed with 3-μm ODS particles with a length of 30 mm and a diameter of 0.8 mm. The results presented herein demonstrate the performance of the pumping system and the column using alkylphenols, catecholamine, catechin, and amino acids.

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Author information

Author notes

  1. Mitsutaka Fujii

    Present address: Quality Assurance Department, Takeda Giken Service, Ltd.,, 4720 Takeda, 743-8502, Mitsui, Hikari, Yamaguchi, Japan

  2. Ichiro Yanagisawa

    Present address: Science Solutions International Laboratory, Inc., 2-21-7 Naka-cho, 153-0065, Meguro, Tokyo, Japan

Authors and Affiliations

  1. Division of Applied Chemistry, Faculty of Engineering, Hokkaido University, Kita 13 Nishi 8, 060-8628, Sapporo, Japan

    Akihiko Ishida, Takehiro Fujimoto, Shunsuke Sasaki, Hirofumi Tani & Manabu Tokeshi

  2. Nano Fusion Technologies, Inc., c/o B-M202 Collaborative Research (CCR) Bldg., Institute of Industrial Science, The University of Tokyo, 4-6-1 Komaba, 153-8505, Meguro, Tokyo, Japan

    Mitsutaka Fujii & Ichiro Yanagisawa

Authors
  1. Akihiko Ishida
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  2. Mitsutaka Fujii
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  3. Takehiro Fujimoto
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  4. Shunsuke Sasaki
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  6. Hirofumi Tani
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  7. Manabu Tokeshi
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Correspondence to Akihiko Ishida.

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Ishida, A., Fujii, M., Fujimoto, T. et al. A Portable Liquid Chromatograph with a Battery-operated Compact Electroosmotic Pump and a Microfluidic Chip Device with a Reversed Phase Packed Column. ANAL. SCI. 31, 1163–1169 (2015). https://doi.org/10.2116/analsci.31.1163

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  • DOI: https://doi.org/10.2116/analsci.31.1163

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