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Construction of a Dimethyl Sulfoxide Sensor Based on Dimethyl Sulfoxide Reductase Immobilized on a Au Film Electrode

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Abstract

A novel dimethyl sulfoxide (DMSO) sensor using DMSO reductase and film electrodes was constructed. The Au and Ag electrodes were fabricated on slide glass by vacuum deposition and the application of a photolithographic technique. The micro-chamber (4 × 50 × 1 mm, volume 200 μl) was fabricated on a poly(dimethylsiloxane) (PDMS) polymer. The Pt electrode was implanted in a PDMS polymer. DMSO reductase was immobilized on a Au film electrode with bovine serum albumin (BSA)-glutaraldehyde. This sensor could determine DMSO in an unpurged aqueous solution with glucose oxidase (GOD) and catalase (CAT) for oxygen removal. The DMSO sensor showed a linear response within 1 mM DMSO with a correlation coefficient of 0.999. The detection limit was 200 μM (3σ), and the sensitivity was 23.8 mA M−1 cm−2. The relative standard deviations at each concentration were within 3.6%.

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Authors and Affiliations

  1. Department of Applied Biological Chemistry, Graduate School of Agricultural and Life Sciences, The University of Tokyo, Yayoi 1-1-1, Bunkyo, Tokyo, 113-8657, Japan

    Hiroki Yonehara, Kiichi Sato, Mitsuru Abo & Etsuro Yoshimura

  2. Institute for Biological Resources and Functions, National Institute of Advanced Industrial Science and Technology (AIST), Central 6, 1-1-1 Higashi, Tsukuba, Ibaraki, 305-8566, Japan

    Shin-ichiro Fujii

Authors
  1. Hiroki Yonehara
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  2. Shin-ichiro Fujii
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  3. Kiichi Sato
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  4. Mitsuru Abo
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  5. Etsuro Yoshimura
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Corresponding author

Correspondence to Mitsuru Abo.

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Yonehara, H., Fujii, Si., Sato, K. et al. Construction of a Dimethyl Sulfoxide Sensor Based on Dimethyl Sulfoxide Reductase Immobilized on a Au Film Electrode. ANAL. SCI. 23, 55–58 (2007). https://doi.org/10.2116/analsci.23.55

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  • DOI: https://doi.org/10.2116/analsci.23.55

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