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A MutS Protein-immobilized Au Electrode for Detecting Single-base Mismatch of DNA

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Abstract

A novel electrochemical biosensor was developed to detect gene mutation by using a DNA-mismatch binding protein: MutS from Escherichia coli. The MutS protein was immobilized onto an Au-electrode surface via complex formation between a histidine tag of the MutS protein and a thiol-modified nitrilotriacetic acid chemically adsorbed on the Au-electrode surface. When a target double-stranded DNA having a single-base mismatch was captured by the MutS protein on the electrode, some electrostatic repulsion arose between polyanionic DNA strands and anionic redox couple ions. Consequently, their redox peak currents on a cyclic voltammogram with the Au electrode drastically decreased, depending on the concentration of the target DNA, according to the redox couple-mediated artificial ion-channel principle. By using this assay, one can detect all types of single-base mismatch and single-base deletion.

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References

  1. R. S. Lahue, K. G. Au, and P. Modrich, Science, 1989, 245, 160.

    Article  CAS  Google Scholar 

  2. K. G. Au, K. Welsh, and P. Modrich, J. Biol. Chem., 1992, 267, 12142.

    Article  CAS  Google Scholar 

  3. T. Shibata, M. Nakayama, M. Murata, Y. Katayama, and M. Maeda, Anal. Sci. (Supplement), 2001, 17, i1371.

    Google Scholar 

  4. M. Sugawara, K. Kojima, H. Sazawa, and Y. Umezawa, Anal. Chem., 1987, 59, 2842.

    Article  CAS  Google Scholar 

  5. M. Sugawara, A. Hirano, P. Bühlmann, and Y. Umezawa, Bull. Chem. Soc. Jpn., 2002, 75, 187.

    Article  CAS  Google Scholar 

  6. H. Aoki, P. Bühlmann, and Y. Umezawa, Electroanalysis, 2000, 12, 1272.

    Article  CAS  Google Scholar 

  7. H. Aoki and Y. Umezawa, Electroanalysis, 2002, 14, 1405.

    Article  CAS  Google Scholar 

  8. H. Aoki and Y. Umezawa, Analyst, 2003, 128, 681.

    Article  CAS  Google Scholar 

  9. Y. Umezawa and H. Aoki, Anal. Chem., 2004, 76, 320A.

    Article  Google Scholar 

  10. M. Murata, K. Yano, S. Kuroki, T. Suzutani, and Y. Katayama, Anal. Sci., 2003, 19, 1569.

    Article  CAS  Google Scholar 

  11. M. Murata, K. Yano, K. Fukuyama, M. Maeda, and Y. Katayama, Bull. Chem. Soc. Jpn., 2004, 77, 525.

    Article  CAS  Google Scholar 

  12. M. Murata, H. Gonda, K. Yano, S. Kuroki, T. Suzutani, and Y. Katayama, Bioorg. Med. Chem. Lett., 2004, 14, 137.

    Article  CAS  Google Scholar 

  13. T. K. Sixma, Curr. Opin. Struct. Biol., 2001, 11, 47.

    Article  CAS  Google Scholar 

  14. T. H. Wu and M. G. Marinus, J. Biol. Chem., 1999, 274, 5948.

    Article  CAS  Google Scholar 

  15. J. Schmitt, H. Hess, and H. G. Stunnenberg, Mol. Biol. Rep., 1993, 18, 223.

    Article  CAS  Google Scholar 

  16. I. Babic, S. E. Andrew, and F. R. Jirik, Mutat. Res., 1996, 372, 87.

    Article  CAS  Google Scholar 

  17. J. Brown, T. Brown, and K. R. Fox, Biochem. J., 2001, 354, 627.

    Article  CAS  Google Scholar 

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Authors and Affiliations

  1. Department of Applied Chemistry, Graduate School of Engineering, Kyushu University, 6-10-1 Hakozaki, Higashi-ku, Fukuoka, 812-8581, Japan

    Aishan Han, Taiki Shibata, Masamichi Nakayama & Mizuo Maeda

  2. Bioengineering Laboratory, RIKEN, 2-1 Hirosawa, Wako, Saitama, 351-0198, Japan

    Tohru Takarada & Mizuo Maeda

Authors
  1. Aishan Han
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  2. Tohru Takarada
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  3. Taiki Shibata
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  4. Masamichi Nakayama
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  5. Mizuo Maeda
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Correspondence to Mizuo Maeda.

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Han, A., Takarada, T., Shibata, T. et al. A MutS Protein-immobilized Au Electrode for Detecting Single-base Mismatch of DNA. ANAL. SCI. 22, 663–666 (2006). https://doi.org/10.2116/analsci.22.663

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  • DOI: https://doi.org/10.2116/analsci.22.663

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