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Simultaneous Determination of Tocopherols and Tocotrienols in Hazelnuts by a Normal Phase Liquid Chromatographic Method

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Abstract

A normal-phase high-performance liquid chromatography (NP-HPLC) method for the determination of tocopherols and tocotrienols in hazelnuts is reported. Three extraction procedures (with and without saponification) were assayed; the best results were obtained with a simple solid-liquid extraction procedure. Chromatographic separation was achieved using an Inertsil 5 SI column using isocratic elution with hexane/1,4-dioxane (95.5:4.5, v/v) at a flow rate of 0.7 mL/min. The effluent was monitored by a series arrangement of a diode-array followed by a fluorescence detector. All compounds were separated in a short period of time (17 min). The method proved to be rapid, sensitive, reproducible and accurate, allowing the simultaneous determination of all vitamin E homologues.

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Authors and Affiliations

  1. REQUIMTE, Servigo de Farmacognosia, Faculdade de Farmdcia, Rua Anibal Cunha, 164, 4099-030, Porto, Portugal

    Joana S. Amaral & Rosa M. Seabra

  2. Escola Superior de Tecnologia e de Gestdo, Instituto Politecnico de Braganga, Quinta de Sta. Apolonia, Apartado 134, 5301-857, Braganga, Portugal

    Joana S. Amaral

  3. REQUIMTE, Servigo de Bromatologia, Faculdade de Farmdcia, Rua Anibal Cunha, 164, 4099-030, Porto, Portugal

    Susana Casal & Beatriz P. P. Oliveira

  4. REQUIMTE, Departamento de Engenharia Quimica, Faculdade de Engenharia, Rua Dr. Roberto Frias, s/n, 4200-465, Porto, Portugal

    Duarte Torres

Authors
  1. Joana S. Amaral
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  2. Susana Casal
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  3. Duarte Torres
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  4. Rosa M. Seabra
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  5. Beatriz P. P. Oliveira
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Correspondence to Joana S. Amaral.

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Amaral, J.S., Casal, S., Torres, D. et al. Simultaneous Determination of Tocopherols and Tocotrienols in Hazelnuts by a Normal Phase Liquid Chromatographic Method. ANAL. SCI. 21, 1545–1548 (2005). https://doi.org/10.2116/analsci.21.1545

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  • DOI: https://doi.org/10.2116/analsci.21.1545

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