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Determination of Atmospheric Nitrobenzanthrones by High-Performance Liquid Chromatography with Chemiluminescence Detection

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Abstract

A method using high-performance liquid chromatography with chemiluminescence detection was developed for analyzing mutagenic nitrobenzanthrone (NBA) isomers in airborne particulates. The method was a modification of our previously described method for analyzing nitropolycyclic aromatic hydrocarbons (NPAHs). The pretreatment and reducing conditions for 1-, 2-, 3- and 10-NBAs were the same as those for NPAHs. In order to separate these NBA isomers, we used a polymeric-type ODS column (Cosmosil 5C-18MS); a mixture of 40% acetonitrile and 60% 10 mM imidazole-HClO4 buffer was employed as the mobile phase at a flow rate of 1 mL/min. The isomers of 1-, 2-, 3- and 10-NBA were determined in chemiluminescence with linear calibration graphs from 0.1 to 4 pmol, from 200 to 4000 pmol, from 1 to 50 pmol and from 10 to 400 pmol, respectively. The detection limits (S/N = 3) of 1-, 2-, 3- and 10-NBA isomers were 0.02 pmol, 35 pmol, 0.3 pmol and 3 pmol, respectively. The method was used to analyze airborne particulates at a heavy traffic site in Kanazawa. 2- and 3-NBAs were detected in the extracts of the particulates, while 1-NBA and 10-NBA were not detected. The atmospheric concentrations of 2- and 3-NBAs were 1.83 pmol/m3 and 24.7 fmol/m3, respectively.

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Authors and Affiliations

  1. Graduate School of Natural Science and Technology, Kanazawa University, 13-1, Takara-machi, Kanazawa, 920-0934, Japan

    Ning Tang, Rina Taga, Ryoichi Kizu & Kazuichi Hayakawa

  2. Faculty of Pharmaceutical Sciences, Kanazawa University, 13-1, Takara-machi, Kanazawa, 920-0934, Japan

    Tetsuyuki Hattori, Akira Toroba, Ryoichi Kizu & Kazuichi Hayakawa

  3. National Institute for Environmental Studies, 16-2, Onogawa, Tsukuba, 305-8506, Japan

    Kenji Tamura

Authors
  1. Ning Tang
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  2. Rina Taga
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  3. Tetsuyuki Hattori
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  4. Kenji Tamura
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  5. Akira Toroba
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  6. Ryoichi Kizu
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  7. Kazuichi Hayakawa
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Correspondence to Kazuichi Hayakawa.

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Tang, N., Taga, R., Hattori, T. et al. Determination of Atmospheric Nitrobenzanthrones by High-Performance Liquid Chromatography with Chemiluminescence Detection. ANAL. SCI. 20, 119–123 (2004). https://doi.org/10.2116/analsci.20.119

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  • DOI: https://doi.org/10.2116/analsci.20.119

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