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Determination of DNA with Cetyltrimethylammonium Bromide by the Measurement of Resonance Light Scattering

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Abstract

A simple assay of DNA was developed based on the measurements of enhanced signals of Resonance Light Scattering (RLS) of cetyltrimethylammonium bromide (CTMAB) by DNA. The enhanced RLS signals, measured by simultaneously scanning the excitation and emission monochromators of a common spectrofluorometer with λex = λem, was optimized for the DNA assay with CTMAB. On the conditions of pH 2.21 and ionic strength 0.002, the enhanced RLS intensity at 470.0 nm, AI, was found to be proportional to the concentration of DNA in the range 0–2.5 μg/ml if 1.5 × 10–5 M CTMAB was used. Limits of determination for calf thymus DNA and fish sperm DNA were 4.9 ng/ml and 9.2 ng/ml, respectively. Synthetic samples were determined with the recovery ratio ranging from 93.2% to 105.1%, and the RSD is lower than 2.7%.

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References

  1. R. Marquet and C. Houssier, Anal. Chem., 1989, 176, 265.

    CAS  Google Scholar 

  2. G. J. Gendimenico, P. L. Bouquin, and K. M. Tramposch, Anal. Biochem., 1988, 173, 45.

    Article  CAS  Google Scholar 

  3. P. C. Pandey and H. H. Weetall, Anal. Chem., 1995, 67, 787.

    Article  CAS  Google Scholar 

  4. P. A. E. Piuno and U. J. Scrull, Anal. Chem., 1995, 67, 2635.

    Article  Google Scholar 

  5. A. N. Glazer and H. S. Rye, Nature, 1992, 359, 589.

    Article  Google Scholar 

  6. Y. X. Ci, Y. Z. Li, and X. J. Liu, Anal. Chem., 1995, 67, 1785.

    Article  CAS  Google Scholar 

  7. C. Z. Huang, Y. F. Li, K. A. Li, and S. Y. Tong, Anal. Lett., 1997, 30, 1305.

    Article  CAS  Google Scholar 

  8. G. M. Perez-Howard, P. A. Weil, and J. M. Beenchem, Biochemistry, 1995, 34, 8005.

    Article  CAS  Google Scholar 

  9. D. Y. Chen and N. J. Dovichi, Anal. Chem., 1996, 68, 690.

    Article  CAS  Google Scholar 

  10. M. Hall, I. Kazakov, and Y. M. Yao, Anal. Biochem., 1999, 272, 165.

    Article  CAS  Google Scholar 

  11. R. J. Fulton, R. L. McDade, P. L. Smith, L. J. Kienker, and J. R. Kettman Jr., Clin. Chem., 1997, 43, 1749.

    Article  CAS  Google Scholar 

  12. R. F. Pasternack, C. Bustamante, P. J. Collings, A. Giannetteo, and E. J. Gibbs, J. Am. Chem. Soc., 1993, 115, 5393.

    Article  CAS  Google Scholar 

  13. R. F. Pasternack and P. J. Collings, Science [Washington, D.C.], 1995, 269, 935.

    Article  CAS  Google Scholar 

  14. C. Z. Huang, K. A. Li, and S. Y. Tong, Anal. Chem., 1996, 68, 2059.

    Google Scholar 

  15. C. Z. Huang, K. A. Li, and S. Y. Tong, Anal. Chem., 1997, 69, 514.

    Article  CAS  Google Scholar 

  16. H. Morimoto, P. A. Ferchmin, and E. L. Bennett, Anal. Biochem., 1974, 62, 436.

    Article  CAS  Google Scholar 

  17. Z. Chen, J. Liu, and D. Luo, “Biochemistry Experiments”, 1994, Chinese University of Sciences and Technology Press, Hefei, 111.

    Google Scholar 

  18. K. B. Jacobson and J. E. Turner, Toxicology, 1980, 16, 1.

    Article  CAS  Google Scholar 

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Authors and Affiliations

  1. Institute of Environmental Chemistry, Southwest Normal University, Chongqing, 400715, P. R. China

    Yuan Fang Li, Ping Feng, Cheng Zhi Huang & Ming Li

  2. Department of Environmental Health, The Third Military Medical University, Chongqing, 400038, P. R. China

    Wei Qun Shu

Authors
  1. Yuan Fang Li
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  2. Wei Qun Shu
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  3. Ping Feng
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  4. Cheng Zhi Huang
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  5. Ming Li
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Li, Y.F., Shu, W.Q., Feng, P. et al. Determination of DNA with Cetyltrimethylammonium Bromide by the Measurement of Resonance Light Scattering. ANAL. SCI. 17, 693–696 (2001). https://doi.org/10.2116/analsci.17.693

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  • DOI: https://doi.org/10.2116/analsci.17.693

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