Abstract
The maturity of the gas chromatography technique belies the continuing search for improved operating methods. Cryogenic methods, applied in many forms, have been used for many years to trap volatile compounds in chromatography. Recent work has demonstrated an alternative approach to band accumulation. A small tube incorporating an internal sleeve cooled cryogenically, placed over a gas chromatography capillary column, can be moved back-and-forward over the column to permit collection and remobilization of focused bands. The movement frequency or timing can be altered to give different operational modes and outcomes. Results demonstrate that peaks can be fully accumulated just prior to a detector then rapidly flushed into the detector, allowing considerable increase in peak height as the peak width diminishes. Applications illustrate that all or selected peaks in a chromatogram can be thus modified by the trap. Column bleed can likewise be collected and then re-eluted, leading to a novel presentation of the molecular constituents of the bleed. With multidimensional gas chromatography, heart-cuts from the first column can be trapped and focused at the head of the second column, then rapidly re-injected into the second column leading to certain advantages. Furthermore, by rapid modulation of the cold trap, a single peak can be cut into a series of separate pulses, either into a detector or into a second column. This allows unusual expression of a chromatographic peak profile, and can be incorporated into a comprehensive GC experiment.
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References
A. Hagman and S. Jacobsson, J. Chromatogr., 448, 117 (1988).
E. Baltussen, H.-G. Janssen, P. Sandra and C. A. Cramers, J. High Resolut. Chromatogr., 20, 385 (1997).
W. Jennings, “Analytical Gas Chromatography”, Academic Press, Orlando, 1987.
D. Kaiman, R. Dills, C. Perera and F. DeWalle, Anal. Chem., 52, 1993 (1980).
T. Noy, P. Fabian, R. Borchers, F. Janssen, C. Cramers and J. Rijks, J. Chromatogr., 393, 343 (1987).
B. A. Ewels and R. D. Sacks, Anal. Chem., 57, 2774 (1985).
L. A. Lanning, R. D. Sacks, R. F. Mouradian, S. P. Levine and L. A. Foulke, Anal. Chem., 60, 1994 (1988).
R. F. Mouradian, S. P. Levine, and R. D. Sacks, J. Chromatogr. Sci., 28, 643 (1990).
C. L. Rankin and R. D. Sacks, J. Chromatogr. Sci., 32, 7 (1994).
“Multidimensional Chromatography: Techniques and Applications”, ed. H. J. Cortes, Chromatographic Science Series, Vol. 50, Marcel Dekker Inc., New York, 1990.
R. M. Kinghorn, P. J. Marriott, and M. Cumbers, J. High Resolut. Chromatogr., 19, 622 (1996).
R. M. Kinghorn, T. Anastasopoulos, and P. J. Marriott, LCGC International, 11, 106 (1998).
J. de Boer, H. J. de Geus and U. A. Th. Brinkman, Environ. Sci. Technol., 31, 873 (1997).
J. Adrian, D. Braun, and H. Pasch, LCGC International, 11, 32 (1998).
H. J. Cortes and L. D. Rothman, Multidimensional High Performance Liquid Chromatography, Chap. 6 in “Multidimensional Chromatography: Techniques and Applications”, ed. H. J. Cortes, Chromatographic Science Series, Vol. 50, Marcel Dekker Inc., New York, 1990.
Z. Y. Liu, S. R. Sirimanne, D. G. Patterson, L. L. Needham and J. B. Phillips, Anal. Chem., 66, 3086 (1994).
J. B. Phillips and J. Z. Xu, J. Chromatogr. A., 703, 327 (1995).
J. B. Phillips and E. B. Ledford, Field Anal. Chem. Technol., 1, 23 (1996).
E. Ledford, J. B. Phillips, J. Z. Xu and G. Blomberg, American Laboratory, June 1996, 22.
J. C. Giddings. Use of Multiple Dimensions in Analytical Separations. Chap. 1 in “Multidimensional Chromatography, Techniques and Applications”, ed. H. J. Cortes, Chromatographic Science Series, Vol. 50, Marcel Dekker Inc., New York, 1990.
P. J. Marriott and R. M. Kinghorn, Anal. Chem., 69, 2582 (1997).
P. J. Marriott and R. M. Kinghorn, J. High Resolut. Chromatogr., 19, 403 (1996).
R. M. Kinghorn and P. J. Marriott, J. High Resolut. Chromatogr., 21, 32 (1998).
Z. Y. Liu and J. B. Phillips, J. Microcol. Sep., 6, 229 (1994).
R. M. Kinghorn, P. J. Marriott, and P. A. Dawes, J. Microcol. Sep., in press.
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Marriott, P.J., Kinghorn, R.M. Modulation and Manipulation of Gas Chromatographic Bands by Using Novel Thermal Means. ANAL. SCI. 14, 651–659 (1998). https://doi.org/10.2116/analsci.14.651
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DOI: https://doi.org/10.2116/analsci.14.651