The effectiveness of bacteriophage KpV289 in treatment of acute pneumonia and a hip infection caused by Klebsiella pneumoniae in mice
Authors / Institutions
A.I.Borzilov /
N.V.Volozhantsev /
O.V.Korobova /
T.I.Kombarova /
V.P.Myakinina /
Valentina M. Krasilnikova /
V.V.Verevkin /
E.A.Svetoch /
I.A.Dyatlov /
The objective. To study the effectiveness of bacteriophage KpV289 on two experimental models of a lethal Klebsiella infection (acute pulmonary infection and hip infection) in outbred mice.Materials and methods. Pulmonary infection was modelled by intranasal instillation to mice of 150 semilethal doses (LD50) of a culture of Klebsiella pneumoniae KPM9 strain, inflammation of soft tissue of the hip in mice with subsequent generalisation of infection was initiated by intramuscular injection of 100 LD50 of the same strain culture.
Results. The experiments we conducted have shown a high therapeutic and preventive effectiveness of phage KpV289. A single intra-abdominal or subcutaneous injection of 100 mln of plaque-forming units of the bacteriophage 1 hour before mice infection ensured survival of not less than 80% of animals, in bacteriophage injection 3 hours after infection for 5 days 100% of the infected mice survived in both models of infection. In a later beginning of treatment of infected animals (after 24 hours) survival of mice was 90% in pulmonary infection and 10% in infection of soft tissues of the hip.
Conclusion. The results we obtained are demonstrative of a marked anti-Klebsiella effectiveness of specific bacteriophages in vivo and good prospects of their use for prevention and treatment of Klebsiella infection.
Key words: Klebsiella pneumoniae, bacteriophage, experimental infection, mouse model, phage therapy.
For citation: Borzilov A.I., Volozhantsev N.V., Korobova O.V., Kombarova T.I., Myakinina V.P., Krasilnikova V.M., Verevkin V.V., Svetoch E.A., Dyatlov I.A. The effectiveness of bacteriophage KpV289 in treatment of acute pneumonia and a hip infection caused by Klebsiella pneumoniae in mice. Infekc. bolezni (Infectious diseases). 2017; 15(3): 48–56. (In Russian).
DOI: 10.20953/1729-9225-2017-3-48-56
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