Preprint Review Version 1 Preserved in Portico This version is not peer-reviewed

Phylogenomic Inference, Divergence-Time Calibration, and Methods for Characterizing Reticulate Evolution

Version 1 : Received: 13 September 2023 / Approved: 13 September 2023 / Online: 14 September 2023 (04:50:27 CEST)

How to cite: Bjornson, S.; Upham, N.; Verbruggen, H.; Steenwyk, J. Phylogenomic Inference, Divergence-Time Calibration, and Methods for Characterizing Reticulate Evolution. Preprints 2023, 2023090905. https://doi.org/10.20944/preprints202309.0905.v1 Bjornson, S.; Upham, N.; Verbruggen, H.; Steenwyk, J. Phylogenomic Inference, Divergence-Time Calibration, and Methods for Characterizing Reticulate Evolution. Preprints 2023, 2023090905. https://doi.org/10.20944/preprints202309.0905.v1

Abstract

Phylogenomics has enriched our understanding of the Tree of Life. Non-vertical modes of evolution—such as hybridization/introgression and horizontal gene transfer—deviate from a strictly bifurcating tree model, mirroring a network-like or reticulate structure. Here, we present an overview of a phylogenomic workflow for inferring organismal histories, calibrating those histories to evolutionary time, and detecting reticulate evolution. Mitigating analytical sources of error facilitates accurate reconstructions of evolutionary history and, in turn, characterization of non-vertical modes of evolution. Workflows and methods discussed herein may aid in the rigorous inference of organismal histories in geologic time and reticulation, providing a clearer understanding of the evolutionary process.

Keywords

phylogenetics; hybridization; introgression; horizontal gene transfer; lateral gene transfer; phylogenetic incongruence; gene-tree-species-tree discordance

Subject

Biology and Life Sciences, Ecology, Evolution, Behavior and Systematics

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