Preprint Article Version 1 Preserved in Portico This version is not peer-reviewed

Allogenic Synovia Derived Mesenchymal Stem Cells for Treat-Ment of Equine Tendinopathies and Desmopathies – The Proof-of-Concept

Inês Leal Reis
,
Bruna Lopes
ORCID logo ,
Patrícia Sousa
ORCID logo ,
Ana Catarina Sousa
ORCID logo ,
Mariana Branquinho
ORCID logo ,
Ana R. Caseiro
ORCID logo ,
Sílvia Santos Pedrosa
,
Alexandra Rêma
,
Cláudia Oliveira
ORCID logo ,
Beatriz Porto
ORCID logo ,
Luís Atayde
ORCID logo ,
Irina Amorim
,
Rui Alvites
ORCID logo ,
Jorge Miquel Silva Santos
,
Ana Colette Maurício
* ORCID logo
Version 1 : Received: 9 March 2023 / Approved: 10 March 2023 / Online: 10 March 2023 (02:53:15 CET)

A peer-reviewed article of this Preprint also exists.

Leal Reis, I.; Lopes, B.; Sousa, P.; Sousa, A.C.; Branquinho, M.; Caseiro, A.R.; Pedrosa, S.S.; Rêma, A.; Oliveira, C.; Porto, B.; Atayde, L.; Amorim, I.; Alvites, R.; Santos, J.M.; Maurício, A.C. Allogenic Synovia-Derived Mesenchymal Stem Cells for Treatment of Equine Tendinopathies and Desmopathies—Proof of Concept. Animals 2023, 13, 1312. Leal Reis, I.; Lopes, B.; Sousa, P.; Sousa, A.C.; Branquinho, M.; Caseiro, A.R.; Pedrosa, S.S.; Rêma, A.; Oliveira, C.; Porto, B.; Atayde, L.; Amorim, I.; Alvites, R.; Santos, J.M.; Maurício, A.C. Allogenic Synovia-Derived Mesenchymal Stem Cells for Treatment of Equine Tendinopathies and Desmopathies—Proof of Concept. Animals 2023, 13, 1312.

Abstract

Tendons and ligaments injuries are frequent in sport horses and humans, representing a great therapeutic challenge. Tissue regeneration and function recovery are the paramount of tendon and ligament lesions management. Nowadays, several regenerative treatments are being developed, based on the use of stem cell and stem cell-based therapies. In the present study, the preparation of equine synovial membrane mesenchymal stem cells (eSM-MSCs) for clinical use - collection, transport, isolation, differentiation, characterization, and application is described. These cells are fibroblast-like and grow in clusters. They retain an osteogenic, chondrogenic and adipogenic differentiation potential. 16 clinical cases of tendonitis and desmitis, treated with allogenic eSM-MSCs and autologous serum are also presented, including their evaluation, treatment and follow-up. The use of autologous serum as a vehicle concern to a decreased immunogenic response after the administration of this combination and to its healing properties, due to the presence of growth factors and immunoglobulins. Most of the cases (14/16), healed in 30 days and presented a good outcome. Treatment of tendon and ligament lesions with the mixture of eSM-MSCs and autologous serum appears to be a promising clinical option for this category of lesions in equine patients.

Keywords

allogenic; cell-based therapies; clinical trials; ligament; mesenchymal stem cells; sport horses; syn-ovial mesenchymal stem cell; tendon

Subject

Biology and Life Sciences, Biology and Biotechnology

Copyright: This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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