The objective of this study is to establish a cryopreservation protocol for shoot apices of hybrid aspen (Populus tremula Å~ P. alba). Cryopreservation was carried out using a vitrification method on shoot apices excised from in vitro cultures. Excised shoot apices were precultured at 23oC for 2 days on hormone-free 1/2 MS medium with 0.4 M sucrose, then treated with liquid 1/2 MS medium supplemented with 0.4 M sucrose and 2.0 M glycerol (a loading solution) at 23oC for 30 min. Then shoot tips were treated with Plant Vitrification Solution 2 (PVS2) or PVS3 at 4oC for 90 min and plunged into liquid nitrogen for 1 hour. Following cryopreservation, they were rewarmed at 42oC for 2 min and washed in liquid 1/2 MS medium supplemented with 1.2 M sucrose at 23oC for 30 min. Survival rate was about 80% when shoot tips were treated with PVS2 for 90 min, however, regrowth rate after 3 weeks was about 20%.