National Academy of Agricultural Sciences (NAAS)
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PRINT ISSN : 2319-7692
Online ISSN : 2319-7706 Issues : 12 per year Publisher : Excellent Publishers Email : editorijcmas@gmail.com / submit@ijcmas.com Editor-in-chief: Dr.M.Prakash Index Copernicus ICV 2018: 95.39 NAAS RATING 2020: 5.38 |
Mungbean is one of the inexpensive sources of vegetable dietary protein in India. It is known to be affected by the several pest and disease. However, among those mungbean yellow mosaic virus (MYMV) is most destructive and has become a major limiting factor for the mungbean cultivation. MYMV infection produced the symptoms like yellow mosaic, complete yellowing, puckering, stunting of flower stalks with wrinkled seed shape and dull seed colour. In this present investigation MYMV disease transmission studies were carried out by three different methods viz., sap inoculation, seed transmission and insect transmission by using different insect vectors viz., whiteflies (Bemisia tabaci), aphid (Aphis craccivora) and mite (Tetranychus spp). Results of the present investigation revealed that none of the plants inoculated with sap, aphids, and mites had characteristic MYMV symptoms. The inoculated with the viruliferous whiteflies showed yellow mosaic symptoms and transmits the disease up to 80 per cent in mungbean plants. The presence or absence of the virus in sap inoculated mungbean plants and presence of virus in body of insect vector was determined by polymerase chain reaction (PCR). The amplification of virus DNA was only observed in case of viruliferous whiteflies while, amplification of virus not observed in other vectors. So that the results of this present investigation have been confirmed that MYMV is transmitted only through the whiteflies and is not transmitted by sap inoculation or any other insect vectors.
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