Efficacy of Cigu Xiaozhi pill（慈菇消脂丸） on non-alcoholic steatohepatitis-associated lipoapoptosis through stress-activated c-Jun N-terminal kinase signalling pathwayEnglish Full Text

MA Yanhua;HAN Tao;YANG Shaojun;YU Chengzu;SHI Xia;WU Yan;College of Clinical Medicine, Gansu University of Chinese Medicine;College of Pharmacy, Gansu University of Chinese Medicine;Guangxi Beihai Hospital of Traditional Chinese Medicine;Center of Teaching experiment, Gansu University of Chinese Medicine;

Abstract: OBJECTIVE: To investigate the efficacy of Cigu Xiaozhi pill（慈菇消脂丸, CGXZ） on non-alcoholic steatohepatitis（NASH）-associated lipoapoptosis through the stress-activated c-Jun N-terminal kinase（JNK）/stress-activated protein kinase signalling pathway.METHODS: Sixty male Sprague-Dawley rats were randomly divided into the following groups（10 rats each）: blank control, model, low-dose CGXZ,medium-dose CGXZ, high-dose CGXZ, and positive control（treated with SP600125, a JNK inhibitor）.The NASH model was established and the histomor-phological characteristics of haematoxylin and eosin-stained liver tissues were examined under a light microscope. Cell apoptosis in liver tissues was assessed via terminal deoxynucleotidyl transferase d UTP nick-end labelling assay. In addition, the m RNA and protein expression levels of p-JNK,p-c-Jun, caspase-8, Fas, and Fas-L were determined via fluorescence-based quantitative real-time PCR,immunohistochemical and Western blot assays.RESULTS: Histopathological examination of the liver showed that the model rats had moderate-to-severe steatosis with infiltration of inflammatory cells as well as significantly higher expression levels of the p-JNK, p-c-Jun, caspase-8, Fas, and Fas-L proteins, compared with those in the blank control group（P ＜ 0.01）. Hepatic lobules of the rats in the treatment groups showed significantly reduced vacuolar degeneration and steatosis as well as alleviated inflammatory cell infiltration. The high and medium-dose CGXZ groups exhibited significantly lower m RNA and protein expression levels of p-JNK, p-c-Jun, caspase-8, Fas, and Fas-L, compared with those in the model group（P ＜ 0.05 or P ＜0.01）.CONCLUSION: CGXZ pill inhibited the onset of hepatocyte apoptosis by regulating the expression of p-JNK, p-c-Jun, caspase-8, Fas, and Fas-L, thereby exerting therapeutic effects against NASH.

Keywords：

Non-alcoholic fatty liver disease; JNK mitogen-activated protein kinases; apoptosis; stress-activated signaling pathway; Cigu Xiaozhi pill;

DOI：
10.19852/j.cnki.jtcm.2021.01.010
Series：
(E) Medicine ＆ Public Health
Subject：
Traditional Chinese Medicinal Herbs
Classification Code：
R285.5

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Efficacy of Cigu Xiaozhi pill（慈菇消脂丸） on non-alcoholic steatohepatitis-associated lipoapoptosis through stress-activated c-Jun N-terminal kinase signalling pathwayEnglish Full Text

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