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Rapid extraction of genomic DNA and its application in detection of genetically modified soybeansChinese Full TextEnglish Full Text (MT)

CHEN Yu;WANG Xiao-fu;CHEN Xiao-yun;PENG Cheng;XU Jun-feng;LI Yue-ying;College of Life Science, Shenyang Normal University;State Key Laboratory for Managing Biotic and Chemical Threats to the Quality and Safety of Agro-products, Zhejiang Academy of Agricultural Sciences;

Abstract: To explore an on-site extraction method for plant genomic DNA during detection of genetically modified(GMO)soybeans, a rapid extraction of plant DNA was developed by applying silicon membrane to absorb DNA, together with filter membrane and syringe. The method was used to extract DNA from leaves and seeds of 5 major crops, including soybean, cotton, rapeseed, corn, and rice. The conventional PCR and basic RPA(recombinase polymerase amplification)are simultaneously used to amplify endogenous genes from the DNA extracted by the rapid extraction and QIAGEN kit methods. Results showed that the rapid extraction method had higher DNA yield than the QIAGEN method, while both of the extraction met the needs of conventional PCR and basic RPA amplification. By detecting the genetically modified soybean SHZD32-1, the DNA rapid extraction method combined with conventional PCR, fluorescence RPA and fluorescence quantitative PCR showed consistent and accurately results. For this developed rapid DNA extraction method does not require centrifuge and other equipment, and could be completed in 3 minutes on site, it was considered to be successfully meeting the requirements on site.
  • DOI:

    10.19802/j.issn.1007-9084.2020301

  • Series:

    (D) Agriculture

  • Subject:

    Crop

  • Classification Code:

    S565.1

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