The paper presents the experimental test results of a common rail injection system operating with biodiesel and the diesel fuel. The three fuel split injection strategies were implemented to investigate the effects made by biodiesel and a fossil diesel fuel on the history of injector inlet pressure and the injection rate. In addition, the three intervals between split injections and the different injection pressures were used to obtain more information about the studied subjects. The obtained results showed that the peak mass injection rates of the main injection phase were slightly higher when using biodiesel than the respective values measured with the normal diesel fuel. Because the first injection phase activated the fuel pressure fluctuations along the high-pressure line and in front of the injector, the time-span between injections has an impact on the injector inlet pressure and thus the fuel injection rate during the second injection phase. Since the nozzle closes little later for biodiesel, the injector inlet pressure also occurred latter in the cycle.