Influence of Different Land Use Types and Soil Depths on Selected Soil Properties Related to Soil Fertility in Warandhab Area, Horo Guduru Wallaga Zone, Oromiya, Ethiopia

Results: Soil physical properties, pH, SOM, total nitrogen, available P, exchangeable Mg, K, Na, CEC and micronutrients observed were significantly affected (P ≤ 0.05) by land use. All land use types were clayey but clay loam for forest land. The highest and lowest mean BD was obtained in subsurface of cultivated and surface layer of grazing land respectively. The highest soil water content at FC and AWHC and lowest was recorded in subsurface and surface of forest and cultivated land, respectively. The highest pH = 6.47 and lowest pH = 5.29, were obtained in subsurface of grass land and surface layers of cultivated land, respectively. The range of pH in surface and subsurface layers of all and use types were strongly acidic to slightly acidic. The higher (16.00, 20.04, 89.03, 2.49, 3.39) mg/kg available P, Fe, Mn, Zn, Cu, respectively and CEC (32.80 cmol(+)/kg) were recorded in surface layer of cultivated land than in subsurface. Values of exchangeable bases (Na, K, Ca and Mg) were lower on surface of cultivated land than subsurface of forest land, respectively.


Introduction
With the rapid growth of African population, soil fertility is a major concern of the world, including Ethiopia which is the second populous African country. High quality soils not only produce better food and fiber, but also help to establish natural ecosystems and enhance air and water quality [1]. Soil fertility changes and the nutrient balances are taken as key indicators of soil quality [2]. It is well known that in traditional farming systems, farmers use bush fallow, plant residues, household refuse, animal manures and other organic nutrient sources to maintain soil fertility and soil organic matter. Soil fertility varies spatially from field to larger region scale, and is influenced by both land use and soil management practices [3]. Revealing spatial variability of soil fertility and its influencing factors are important to improve sustainable land use strategies [4]. It is reported that differences in fertilization, cropping system and The physical conditions and variations in altitude have resulted in a great diversity of climate, soil and vegetation Asrat [6] which constitutes the high mountains, deep gorges, flattopped plateaus, and rolling plains of Ethiopian topographical feature. Due to such typical feature and cultivation pattern such as on steep and fragile soils with inadequate investments in soil conservation, erratic and erosive rainfall patterns, declining use of fallowing, limited recycling of dung and crop residues to the soil, limited application of external sources of plant nutrients, deforestation and overgrazing Belay [7] and Hurni [8] were the main causes of land degradation in Ethiopia. Inappropriate use of land, mainly characterized by extensive deforestation and conversion into agricultural land, is the most widespread change in land use in Ethiopia [9,10]. All these contribute to the change in chemistry, biology and hydraulics of the soil.
Smith et al. [11] reported that deforestation and cultivation of the same land as the main cause of changes in soil pH and acidifications. Although soil organic matter (SOM) are crucial in regulating the supply of plant nutrients, water flow and determines the physical properties of the soil Cotrufo et al. [12], its altered by soil managements and types, land uses systems and types and the climate [13][14][15]. As reported by Genxu et al. [16] the availability and distributions of P in soil profile is determined by the different land use types, management practices such as level of SOM, biomass production in the soil, vegetation cover and nutrient cycling in the ecosystems. In another study, in the sub-humid highlands of South-western Ethiopia, shift of land use changes from natural forest to cultivation led to depletion of P [9]. Because of the study area are facing agricultural challenges such as shortage of land for crop cultivation and livestock grazing, decline of soil fertility and rainfall variability resulting in low yield production, determining the soil property is crucial for further soil management and improvement. Therefore, this study was initiated with the objective to investigate the influence of different land use types and soil depths on selected soil physical, chemical properties and organic matter in Warandhab areas of Jimma Rare District.

Description of the Study Area
The study was conducted at Warandhab area in Jimma Rare District, Horo Guduru Wallaga Zone, Oromiya Regional State. It is about 255 km away from the capital, Addis Ababa, and located in the mid-west of Ethiopia and 10 km away from the district town, Wayu, to the west. Geographically, it is located between

Climate
The average weather data recorded at the weather station located at Wayu town near the study area from the year 2004-2010 indicates that the study area has a uni-modal rainfall pattern with mean annual rainfall of 1530.9 mm. The rainy season covers the period from mid-April to October and the maximum rain is received in the months of June, July and August

Site Selection, Soil Sampling and Preparation
Planning, surveying and appropriate sampling are important considerations when attempting to measure changes in surface soil chemical and physical properties to accommodate spatial variation. Primarily, a general visual field survey of the area

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was carried out to have a general view of the variations in the study area. Representative soil sampling site were then selected based on vegetation cover and cultivation history. Following this, three representative land uses (cultivated, forest and grass lands) were selected and Global Positioning System (GPS) and clinometers were used to identify the geographical locations and slopes of the sampling sites, respectively. Using Geographical Information System (GIS) and geographical coordinates for each sampling site, the sampling site was sketched. Composite soil samples were collected from the depths of 0-20 and 20-40 cm. Each composite soil samples was made from 5-10 subsamples collected from within the respective area delineated as a replication of such land use. Dead plants, furrow, old manures, wet spots, areas near trees and compost pits were excluded during collection of samples. This was minimizing differences, which may arise because of the dilution of SOM due to mixing through cultivation and other factors.
The soil samples collected from representative land uses with its replications were then air-dried, mixed well and passed through a 2 mm sieve for the analysis of selected soil physical and chemical properties. Separate soil core samples from the 0-20 and 20-40 cm depths were taken with a sharp-edged steel cylinder forced manually into the soil for bulk density determination. To make one composite soil sample the subsamples were mixed well and about 1 kg of the mixed subsamples was properly labeled. Finally eighteen total composite soil samples were prepared and packed in a plastic bowl, and transported to Soil Testing Centre for further analysis.

Analysis of Soil Properties
Soil texture was determined by the Bouyoucos hydrometer method after destroying organic matter and dispersing the soil by using sodium hexametaphosphate as described by [17]. Bulk density was determined from undisturbed soil samples by the core method after drying a defined volume of soil in an oven at 105 °C to constant weight [18]. It was calculated as the ratio of mass of oven dried soil to the volume of the sampling core. The soil water content at PWP and FC was determined after soils were subjected to required pressures (15 and 1/3 bars, respectively) by the pressure plate apparatus. Soil pH (H 2 O) and pH (KCl) were measured by using a pH meter in a 1:2.5 soil: water and soil: KCl ratios, respectively [19]. Soil organic carbon was estimated by the Walkley-Black wet oxidation method and converted to organic matter by multiplying the percent organic carbon content by a factor of 1.724, assuming that organic matter is composed of 58% carbon [20].
Total nitrogen was determined by the micro-Kjeldahl digestion, distillation and titration method Sahlemedhin and Taye [21] and available P was determined using the standard Olsen extraction method [22]. Total exchangeable bases were determined after leaching the soils with ammonium acetate [23]. Amounts of Ca 2+ and Mg 2+ in the leachate were analyzed by atomic absorption spectrophotometer and K + and Na + were analyzed by flame photometer. Cation exchange capacity was determined at soil pH level of 7 after displacement by using 1N ammonium acetate method in which it was estimated titrimeterically by distillation of ammonium that was displaced by sodium [24]. Percent base saturation was calculated by dividing the sum of the base forming cations (Ca, Mg, Na, and K) by the CEC of the soil and multiplying by 100. Total exchangeable acidity was determined by saturating the soil samples with potassium chloride solution and titrated with sodium hydroxide as described by [25]. Extractable micronutrients (Fe, Cu, Zn, and Mn) were extracted by diethylene triamine penta acetic acid (DTPA) as described in [21]. Finally, the amounts of all these micronutrients were measured by atomic absorption spectrophotometer at their respective wave lengths.

Statistical Analysis
The general linear model (GLM) ANOVA procedure of statistical analysis system SAS [26] was used for performing the significance of differences in soli parameters. A post hoc separation of means was done by least significant difference (LSD) test after main effects was found significant at P≤0.05. The analysis was performing for each land use types (cultivated, grass and forest lands) in six combined treatments.

Soil texture
The sand and clay fractions were significantly (P ≤ 0.01) affected by the interaction of land use and soil depth. Similarly, the silt fraction was significantly (P≤ 0.01) affected by land use and soil depth (Table 1). Considering the interaction effects of land use and soil depth, the highest (51%) sand and (31%) silt contents were recorded at the surface layer of forest land than cultivated land. In contrast, the highest (58%) clay content was recorded at the subsurface layer of the cultivated land, whereas the lowest (18.00) clay content was observed in the surface layer of the forest land ( Table 1). The current result is in agreement with the findings of Shiferaw [27] who reported an increase in clay content with depth under cultivated lands due to long period of cultivation.
In a similar way Boke [28]; Alemayehu and Sheleme [29] reported that high sand content in grass land soils in Southern Ethiopia. Buol [30] also observed that the accumulation of clay in the subsurface horizon could also be contributed by the in situ synthesis of secondary clays or the residual concentration of clays from the selective dissolution of more soluble minerals of coarser grain size in the B horizon. Agoume and Birang [31] similarly found that land-use systems and soil depths significantly affected the sand, the clay and the silt fractions of the soils size distributions in Cameroon and even by land use alone Jaiyeoba, VoundiN, kana and Tonye [32][33][34][35][36] reported that

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continuous cropping and intensive land use affected the particle size distribution and that these changes related to cultivation time, but the current finding is in contradict to the result reported by Shepherd et al. [35] who found that land use systems were no effect on soil particles. Sand and silt content decrease while clay content increases across depth from surface to subsurface soils. The increase in clay contents with depth under all land use types may be due to translocation of clay from surface to subsurface layers, which ultimately increase the proportion of sand and silt contents in the surface soil layers.

Soil water characteristics
Water retention at both FC and PWP was significantly affected by main effects (P ≤ 0.01) and their interaction (Table  1). Moreover, AWHC was significantly (P ≤ 0.01) affected by land use and soil depth but not significantly (P ˃ 0.05) affected by their interactions (Table 1). Significant difference in FC and PWP due to the interaction of land use and soil depth as observed in the study area was high at subsurface layers of the forest and cultivated lands and low at the surface layers of the cultivated and grass lands, respectively (Table 2). On the other hand, the highest (17.98%) and the lowest (9.67%) AWHC among the land use types was obtained in the forest and cultivated lands, respectively. The soil water content at FC, PWP and AWHC increased with soil depth ( Table 2). The result of this study is in agreement with Wakene [37] and Ahmed [38] who reported that soil water content at FC, PWP and AWHC were found to increase with depth for soils under different management practice. As per AWHC rating developed by Beernaert [39], the AWHC of the surface soils of the study area was in the range of low in cultivated land to medium in forest land (Table 2). Moreover, various studies also examined the effects of land use and land cover change on soil physico-chemical properties [31,32,[39][40][41]. *Main effect means within a column followed by the same letter are not significantly different from each other at p ≤ 0.05 LSD = least significant difference; SEM = standard error of the mean; BD = bulk density; FC = field capacity; PWP = permanent wilting point; AWHC = available water holding capacity.

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Bulk density value was significantly (P ≤ 0.05) affected by land use types ( Table 2). The highest (1.41 g/cm 3 ) mean value of bulk density was recorded on the cultivated land and the lowest (1.11 g/cm 3 ) mean value under the grass land (Table 2).
Compaction resulting from intensive cultivation might have caused the relatively higher bulk density values in the surface soil layers of the cultivated land than that of the respective soil depths in the grass land. Liu [5] and Celik, [36] reported that land use and soil management practices influence the soil nutrients and related soil processes, such as erosion, oxidation, mineralization and leaching etc. Moreover, in non-cultivated land, the type of vegetative cover is a factor influencing the soil organic carbon content as reported by Liu et al. [5]. Land use change also produces considerable alterations Fu et al. [42] and usually soil quality diminishes after the cultivation of previously untilled soils [38]. The reason for the relatively low soil bulk density on the grass and forest lands as well as surface soil layer could be due to the highest SOM content and low clay content, respectively. Similarly, Gol [43] also investigated the effects of land use change on soil properties and organic carbon at Dagdami river catchment in Turkey and bulk density as affected by land use in Ethiopia [40]. *Main effect means within a column followed by the same letter are not significantly different from each other at p ≤ 0.05; NS = not significant; STC = soil texture class; c = Clay; cl = Clay loam; BD = Bulk density; FC = Field capacity; PWP = Permanent wilting point; AWHC = Available water holding capacity; LSD = least significant difference; SEM = Standard error of the mean; CV = Coefficient of variation. Table 3: Interaction effects of land use and soil depth (cm) on some chemical properties of soils in Warandhab area.  Soil pH values measured in a suspension of soil to water ratio are greater than that of in soil to KCl solution ratio. The pH (H 2 O) value of the soils content was significantly (P ≤ 0.01) affected by all land use types and their interaction effects ( Table  3). The highest (6.47) and the lowest (5.29) soil pH-H 2 O values were recorded under the grass and the cultivated lands at 20-40cm and 0-20cm soil depths, respectively (Table 3). Continuous cultivation practices, excessive precipitation, and application of inorganic fertilizers could be some of the factors which are responsible for the variation in pH in the soil profiles [42][43][44][45]. In line with the findings of this study, soil pH increased with depth of soil profile and relatively high pH was observed at subsoil horizons in Alfisols of Bako area Wakene [37] and in Vertisols of the central highlands of Ethiopia [46]. Agoume and Birang [31] also reported that pH of the soil as affected by land use system of an Oxisol in the Humid Forest zone of the Southern Cameroon. In other study conducted by Nega and Heluf [47] they found that pH the soil was affected by the interactions of land use changes and the soil depths in Western Ethiopia. Alemayehu and Sheleme [29] also found an increment of soil pH at two depths (0-15 and 15-30cm) under enset cultivations and. Generally, the pH (H 2 O) values observed in the study area were within the ranges of moderately acidic to slightly acidic (5.50-6.23) and pH (KCl) values ranged from very strongly acidic to strongly acidic (4.30-5.21) soil reactions as classifications indicated by Brady and Weil [48].

Soil Chemical Properties
Soil organic matter/SOM content was significantly (P ≤ 0.01) affected by the interaction of land use type with soil depth ( Table 3). The interaction effect of land use by soil depth, on the variability of SOM was significantly higher (8.37%) at surface layer of the forest land and lower (1.83%) at subsurface layer of cultivated land ( Table 3). The reason may be due to intensive cultivation of the land and the total removal of crop residues for animal feed and source of energy. Based on the distribution of SOM ranges suggested by Berhanu [49], the soils of the study area were ranged from medium in cultivated land to very high in forest land. Urioste et al. [50] suggests thatroots of the grass and fungial hyphae are probably responsible for the high amount of total organic matter in grassland. This result is in agreement with Eylachew [51,52] and who reported that SOM content under grazing and cultivated soils were lower than those under natural vegetation's/forest. Malo et al. [53]; Nega and Heluf [47] also reported less organic carbon in the cultivated soils than grassed soils and high in the surface soils of forest land while least were from subsurface layers of the cultivated soils, respectively.

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Total nitrogen content of soils was significantly (P ≤ 0.01) affected the interaction of land use by soil depth (Table 3). On the other hand, carbon to nitrogen (C/N) ratio of the soils at the study area was significantly affected by the interaction of land use with soil depth (P ≤ 0.01). On the other hand, it was not significantly (P > 0.05) affected by soil depth ( Table 3). The effect of land use by soil depth on total N was significantly higher (0.42%) at the surface layer of the forest land than (0.09%) in the subsurface layer of the cultivated land ( Table 3). The mean total N content of the surface soils of the study area was within the range of low in soils of cultivated land to very high in soils of forest land as per total N rating suggested by Berhanu [49,47]. The very high total N content in soils of the forest land could be associated with the high available P and CEC contents of these soils. In their study,Alemayehu and Sheleme [29] also found higher total nitrogen in grassland fields followed by that of enset at 0-15 and 15-30cm soil depths. Moreover, this study was in agreement with Ukaegbu and Akamigbo [54]; Agoume and Birang [31] and Iwara et al. [55] they found that total nitrogen as affected by land use systems.
Carbon to nitrogen ratio of the subsurface layer of the cultivated land was significantly higher (11.65) than those under forest and grazing lands at 20-40cm soil depths ( Table 3). The C/N ratios were numerically high in the subsurface than surface soil layers. Our current result contradicts the finding of Nega and Heluf [47] who found that land uses did not variant in C/N ratio but these ratios varied across soil depth. Yihenew [56] indicated that the optimum range of the C/N ratio is about 10:1 to 12:1 that provides nitrogen in excess of microbial needs. Accordingly, the C/N ratio of the soil across the study area may be considered to be within the optimum range in all land use types and soil depth. *Main effect means within a column followed by the same letter are not significantly different from each other at p ≤ 0.05 LSD = least significant difference; SEM = standard error of the mean; SOM = soil organic matter; total N = total nitrogen; C/N = carbon to nitrogen ratio; AvP = available phosphorus; EA = exchangeable acidity.

Available Phosphorus
The available phosphorus (P) was significantly (P ≤ 0.01) affected by the interaction of the two factors (Table 3 and Appendix I). The content of available P in the cultivated land appeared to be significantly higher than the other two land use types. The higher in available P contents in soils of cultivated land were due to continuous application of mineral P fertilizer for few years as indicated by different farmers in the area. Van der Eijk et al. [57] have also reported that the high content of P under maize farms than of grass land soils could be due to the continuous application of phosphorus fertilizer applications. Similarly, Boke [28] also found that high availability of P under enset farms which is due to rapid mineralization and additions of manure and crop residue. Accordingly, by considering the interaction effect of land use with soil depth, the highest (16.00 mg kg -1 ) and the lowest (1.67 mg kg -1 ) available P contents were recorded at the surface soil layer of the cultivated and subsurface soil layer of the grass lands, respectively (Table 3). This result is in agreement with the findings reported by Nega and Heluf [47]; Ekukinam [58]; Alemayehu and Sheleme [29] that soil available P was significantly affected by land use types. The mean available P content of the soils of the study area was within the range of low in soils of grass land to high in soils of cultivated land as per available P rating suggested by [22].

Exchangeable Acidity and Basicity
The exchangeable acidity was not significantly (P > 0.05) affected by land use and soil depth interaction (Table 3). Considering the absolute figures, relatively higher EA was recorded in soils of the cultivated land as compared to the other land use types (Table 3). These results show that intensive cultivation and application of inorganic fertilizers leads to the higher exchangeable acidity content under the crop field than the other land uses.
The content of exchangeable calcium (Ca) was not significantly (P ˃ 0.05) affected by the interaction of land use with soil depth (Table 4). Based on the data obtained in the study area, relatively higher exchangeable Ca was recorded in subsurface soil layer of the forest land as compared to the other land use types and their depths (Table 4). According to the rating set by Landon [59], the Ca contents of soils in the study area ranged from high in surface cultivated land to very high in subsurface forest land ( Table 4). The present study was in contradicted with Agoume and Birang [31]; Iwaraet al. [55]; Gebeyaw [32] who reported exchangeable calcium (Ca) was significantly affected by land use systems. However, Alemayehu and Sheleme [29] found higher Ca exchangeable under enset field at 0-15cm soil depths.
Exchangeable K content was significantly (P ≤ 0.01) affected by the interaction of land use and soil depth (Table 4). Considering the interaction effects of land use by soil depth, the highest (2.15 cmol(+)/kg) and the lowest (0.77 cmol(+)/ kg) exchangeable K contents were recorded at the subsurface layers of the forest land and the surface layers of the cultivated land, respectively ( Table 4). The low exchangeable K contents observed under cultivated land could probably due to continuous cultivations and inorganic farming practices in the study area which is supported by previous findings that indicate intensity of weathering, cultivation and use of acid forming inorganic fertilizers affect the distribution of K in the soil system and enhance its depletion [53].
The concentration of exchangeable potassium (K) followed trend of being enset field > grass land >maize farms for the three land uses and depths (0-15 and 15-30cm) and highest cation exchange capacity (CEC) under grassland [29].With the exceptions of the surface layers of the forest land and the subsurface layer of the grass lands, the mean exchangeable K contents of the remaining treatment combinations were

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significantly different (P ≤ 0.05) from each other due to the interaction effects. The rate of mean exchangeable K values observed in this study ranged from high in cultivated land to very high in forest land Wang et, al. [60,61] (Table 4). Urioste et al. [50] reported that, the addition of organic matter increases the amount of exchangeable cations bases and the low cations bases in cultivated fields are due to the intensive cultivations and continues use of inorganic fertilizers which enhances the loss of base cations through erosion, crop harvest and leaching [62]. Table 4: Interaction effects of land use and soil depth (cm) on exchangeable basic cations and CEC of soils in Warandhab area.  The content of exchangeable Na was significantly (P ≤ 0.01) affected by the interaction of land use by soil depth ( Table 4). The effects of land use by soil depth on exchangeable Na was significantly high (0.25 cmol (+) /kg) under subsoil layer of the forest land and low (0.13 cmol (+ )/kg) under surface soil layer of the cultivated land (Table 4). According to the rating set by Landon [59], the Na contents of soils in the study area is low. Alemayehu and Sheleme [29] reported higher exchangeable Na exists in the 15-30cm depth in grassland soils under enset field.The increase in basic cations concentration as well as percent base saturation with depth may suggest the existence of downward movement of these constituents exchangeable Ca, Mg, Na and K within the profile. Generally, the lower available exchangeable Na cation under cultivated land is an indicative for the depletion of the surface soils of the study area. Moreover, Negassa [62] reported that intensive cultivation and continuous use of inorganic fertilizers in the cultivated fields that will enhance loss of base cations through leaching, erosion and crop harvest.

Cation Exchange Capacity
The CEC values of the soils in the study area were significantly (P ≤ 0.01) affected by the interaction of land use with soil depth (Table 4). Significant difference in CEC contents due to the interaction of land use and soil depth was observed in the study area as highest (39.00 cmol (+) /kg) in surface soil layer of the grass land and lowest (23.87 cmol (+) /kg) in subsurface soil layer of the cultivated land. CEC values decreased from the surface to the subsurface layer under different land use types (Table 4). Based on CEC ratings developed by Landon [59], the CEC content of soils of the study area was rated as high in their CEC. It was generally low in the cultivated land than in the other land use types ( Table 4). As indicated by Mesfin [63]; Negassa [62]; Boke [28] the depletion of exchangeable bases as the result of intensive cultivation and application of acid forming inorganic fertilizers which reduced the CEC under the cultivated land. Reid and Dirou [64] reported that oils with large amounts of clay or OM have higher exchange capacities than sandy soils, which are usually low in organic matter. Alemayehu and Sheleme [29]; Wasihun et al. [40] also reported high cation exchange capacity (CEC) values under grassland and grazing land compared to cultivated land ,respectively.
Exchangeable magnesium (Mg) was significantly (P ≤ 0.01) affected by land use, but not significantly (P ˃ 0.05) affected by soil depth and the interaction of land use with soil depth. The mean values of exchangeable magnesium (Mg) was higher (8.51 cmol (+) /kg) under the forest land and lower (3.62 cmol (+) /kg) under the cultivated land. As per exchangeable Mg rating set by Landon [59], the Mg contents of soils in the study area was in the range of high in cultivated land to very high in forest land ( Table  5). The result of study was in agreement with those reported by Wakene [41], who reported that inorganic fertilizer application is the root cause of soil acidity. In addition, Wang et, al. [60]; Iwara et al. [55] also indicated that climate and geological history are the main important factors affecting soil properties.

Extractable micronutrients (Fe, Mn, Zn and Cu)
The contents of extractable micronutrients (Zn, Mn and Cu) were significantly (P ≤ 0.01) affected by the interaction of land use by soil depth (Table 6), while Fe was significantly (P ≤ 0.01) affected by land use and soil depth, but not significantly (P ˃ 0.05) affected by the interaction of land use with soil depth ( Table 7). As to the ratings of Sims and Johnson the critical level of soil available (DTPA extractable) Fe, Cu, and Mn are 2.5-4.5, 0.1-2.5 and 1-50 mg/kg, respectively. Therefore, the soil contents of extractable micronutrients in all land use types with depth were above the critical levels indicating that there is no deficiency of these micronutrients in the study area (Table 6). According to the report of Alemayehu and Sheleme [29]; Wasihun et al. [40] in that micronutrient status was significantly influenced by different land use systems and soil depth. Accordingly, the contents of all these micronutrients were higher at the surface (0-20 cm) layer than in the subsoil layer of all land use types (Tables 6 & 7). This is due to the lower contents of exchangeable bases in the surface layer which is decreased as the result of leaching. In study conducted by Wasihun et al. [40] at Itang-Kir Area of Gambella Region, Ethiopia, higher extractable micronutrient cations (Fe, Mn, Zn and Cu) were available in grazing land use compared to cultivated land. Laiho et al. [65] also studied the variability in extractable micronutrient (Fe, Mn and Zn) within floristically defined peat land sites. Considering the main effects of land use, the highest contents of Fe (19.74mg/kg), Mn (84.04 mg/kg), Zn (1.87 mg/kg), and Cu (3.03 mg/kg) were recorded under the cultivated land, while the lowest Fe (15.53mg/kg), Mn (47.55 mg/kg), Zn (1.38 mg/kg), and Cu (1.51 mg/kg) were observed under the grass land (Table 7). *Main effect means within a column followed by the same letter are not significantly different from each other at p ≤ 0.05 LSD = least significant difference; SEM = standard error of the mean; CV = coefficient of variation.

Conclusion
The result of this finding suggests that the textural class of cultivated and grass land were clayey, whereas it was clay loam for forest land. The mean bulk density value of cultivated land was significantly greater than the value in the forest and grass land. Soil water content were highly significantly affected by land use and soil depth whereas the mean exchangeable Mg and K content of the soil were highly significantly affected by land use but not by soil depth. On the other hand, Ca was not affected by both land use and soil depth. The mean value of exchangeable Na, K, Ca and Mg were relatively lower in the surface and higher in the subsurface soil layers of cultivated and forest lands, respectively. The value of exchangeable bases relatively increases with soil depth. The mean CEC content of the soil were highly significantly affected by both land use and soil depth. However, it was greater in the surface grass land and lower in subsurface cultivated land. The values of available Fe, Zn and Cu observed were highly significantly affected by land use and soil depth while the mean value of available Mn was highly significantly affected by land use but not with soil depth.