Indian Journal of Animal Research

  • Chief EditorK.M.L. Pathak

  • Print ISSN 0367-6722

  • Online ISSN 0976-0555

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Herbal Mixtures based on Indian Medicinal Plants in Growing Lambs and Ruminal Environments

P.B. Razo-Ortíz, P.A. Hernández-García, G.D. Mendoza-Martínez, J.A. Martínez-García, G. Vázquez-Silva, C. Díaz-Galván, J.J. Ojeda-Carrasco, E. Espinosa-Ayala
Background: The prohibition of the use of antibiotics in lamb feeding requires the search for natural alternatives that can improve productive performance and ruminal fermentation parameters. Herbal mixtures can function as cost-effective substitutes for growth promoters since an extraction or purification process is not required.
Methods: An experiment was conducted to evaluate the effect of Chebulic myrobalan, Terminalia bellirica and Azadirachta indica on the productive performance of finishing lambs. Thirty-six Hampshire x Suffolk male lambs (initial body weight (BW) 23.52±3.67 kg), in individual crates, were used for 45 days of evaluation after 15 days adaptation and were randomly assigned to treatments which consisted of dietary inclusion of the feed plant additive at 0.000, 0.250, 0.375 and 0.500% of dry matter (DM). Rations were sampled for DM, ash and crude protein (AOAC, 2005), neutral and acid detergent fibre (according to van Soest, 1991) analysis and metabolizable energy was estimated. The animals were weighed on days 1, 15 and 45 before feeding and the average daily gain (ADG) and feed conversion rate were calculated by daily recording of the dry matter intake. Contrasts no orthogonal were used to test linear or quadratic effects of the herbal mixture with initial body weight (BW) as a covariate for ADG and final BW.
Result: There was no effect (P>0.05) of herbal mixtures on ADG, final BW, feed efficiency, DM intake and feed conversion by phytobiotic dietary inclusion. Ruminal pH (P=0.07) increased quadratically in response to the herbal mixture dietary concentration. The acetate increased and propionate reduced, both quadratically (P<0.05). The evaluated doses of the polyherbal additive did not influence growth and feed efficiency of lambs. 

  1. Association of Analytical Chemists (AOAC). (2005). Official Methods of Analysis (18th Edn.). Washington DC, USA. pp. 128.

  2. Bhatta, R., Baruah, L., Saravanan, M., Suresh, K.P. and Sampath, K.T. (2012). Effect of medicinal and aromatic plants on rumen fermentation, protozoa population and methanogenesis in vitro. Journal of Animal Physiology and Animal Nutrition. 97(3): 446-56.

  3. Budan, A., Tessier, N., Saunier, M., Gillmann, L., Hamelin, J., Chicoteau, P., Richomme, P. and Guilet, D. (2013). Effect of several saponin containing plant extracts on rumen fermentation in vitro, Tetrahymena pyriformis and sheep erythrocytes. Journal of Food, Agriculture and Environment. 11(2): 576-582.

  4. Cardinali, R., Cullere, M., Dal Bosco, A., Mugnai, C., Ruggeri, S., Mattioli, S., Castellini, C., Trabalza-Marinucci and DalleZotte, A. (2015). Oregano, rosemary and vitamin E dietary supplementation in growing rabbits: Effect on growth performance, carcass traits, bone development and meat chemical composition. Livestock Science. 175: 83-89.

  5. Cardozo, P.W., Calsamiglia, S., Ferret, A. and Kamel, C. (2006). Effects of alfalfa extract, anise, capsicum and a mixture of cinnamaldehyde and eugenol on ruminal fermentation and protein degradation in beef heifers fed a high concentrate diet. Journal of Animal Science. 84: 2801-2808.

  6. Celia, C., Cullere, M., Gerencsér, Z., Matics, Z., Tasoniero, G., Dal Bosco, A., Giaccone, V., Szendrõ, Z. and DalleZotte, A. (2016). Effect of pre-and post-weaning dietary supplementation with Digestarom® herbal formulation on rabbit carcass traits and meat quality. Meat Science. 118: 89-95. https:/ /doi.org/10.1016/j.meatsci.2016.03.022.

  7. Council for International Organizations of Medical Sciences (CIOMS). (2012). International Guiding Principles for Biomedical Research Involving Animals. Available from: http://www.cioms.  Accessed: Sep, 11, 2018. 

  8. Crosby, M., Mendoza-Martínez, G.D., Relling, A., Vázquez, V.A., Lee-Rangel, H.A., Martínez-García, J.A. and Oviedo, M. (2017). Influence of supplemental choline on milk yield, fatty acid profile and postpartum weight changes in suckling ewes. Journal of Dairy Science. 100(Suppl 2): 125.

  9. DalleZotte, A., Celia, C. and Szendrõ, Z. (2016). Herbs and spices inclusion as feedstuff or additive in growing rabbit diets and as additive in rabbit meat: A review. Livestock Science. 189: 82-90. 

  10. Erwin, E.S., Marco, G.J. and Emery, E.M. (1961). Volatile fatty acid analysis of blood and rumen fluid by gas chromatography. Journal of Dairy Science. 44: 1768-1771.

  11. Greathead, H. (2003). Plants and plant extracts for improving animal productivity. Proceedings of the Nutrition Society. 62: 279-290. 

  12. Gupta, V.P., Kamra, D.N., Agarwal, N. and Chaudhary, L.C. (2017). In vitro evaluation of calcium nitrate and a blend of plants containing secondary metabolites for their impact on methanogenesis and feed fermentation. Animal Nutrition and Feed Technology. 17(3): 399-408.

  13. Heidarian-Miri, V., Tyagi, A.K., Ebrahimi, S.H. and Mohini, M. (2013). Plant extract enhanced ruminal CLA concentration, in vitro. Journal of Animal and Feed Sciences. 22(3): 219-228.

  14. Jouany, J.P. and Morgavi, D.P. (2007). Use of ‘natural’ products as alternatives to antibiotic feed additives in ruminant production. Animal. 1(10): 1443-1466. 

  15. Khiaosa-Ard, R., Bryner, S.F., Scheeder, M.R.L., Wettstein, H.R., Leiber, F., Kreuzer, M. and Soliva, C.R. (2009). Evidence for the inhibition of the terminal step of ruminal á-linolenic acid biohydrogenation by condensed tannins. Journal of Dairy Science. 92(1): 177-188. 

  16. Lillehoj, H., Liu, Y., Calsamiglia S., Fernandez-Miyakawa, M.E., Chi, F., Cravens, R.L., Oh, S. and Gay, C.G. (2018). Phytochemicals as antibiotic alternatives to promote growth and enhance host health. Veterinary Research. 49(1): 1-18. 

  17. Martínez-Aispuro, J.A., Mendoza-Martinez, G.D., Cordero-Mora, J.L., Ayala-Monter, M.A., Sánchez-Torres, M.T., Figueroa- Velasco, J.L., Vázquez-Silva, G. and Gloria-Trujillo, A. (2019). Evaluation of an herbal choline feed plant additive in lamb feedlot rations. Revista Brasileira de Zootecnia. 48: 11.  https://doi.org/10.1590/rbz4820190020.

  18. Mejia-Delgadillo, M.A., Lee-Rangel, H.A., Hernandez-Garcia, P.A., Vazquez-Valladolid, A., Mendez-Cortes, H., Guerra-Liera, J.E. and Lopez-Inzunza, H.J. (2021). Effect of a polyherbal additive on performance and parasite infection of Hair Creole Ewes. Indian Journal of Animal Research. 1: 5. https://doi.org/10.18805/ IJAR.B-1370.

  19. Mendoza-Martínez, G.D., Oviedo, M.F., Pinos, J.M., Lee-Rangel, H.A., Vázquez, A., Flores, R. and Pérez, F. (2019). Milk production in dairy cows supplemented with herbal choline and methionine. Revista de la Facultad de Ciencias Agrarias. Universidad Nacional de Cuyo. 52(1): 332-343.

  20. Mirman, D. (2016). Growth Curve Analysis and Visualization using R. Chapman and Hall/CRC The R Series. CRC Press. Taylor and Francis Group.

  21. Misra, S.K. and Agrawala, S.K. (2005). Clinical evaluation of HerbMix in liver disorders, ketosis and other conditions. Livestock International. 11-15.

  22. National Research Council (NRC). (2007). Nutrient Requirements of Small Ruminants. Sheep, Goats, Cervids and New World Camelids. National Academy Press, Washington, DC.

  23. Ogbuewu, I.P., Odoemenam, Y.U., Obikaonu, H.O., Opara, M.N., Emenalom, O.O., Uchegbu, M.C., Esonu, B.O. and Iloeje, M.U. (2011). The growing importance of Neem (Azadirachta  indica A. Juss) in agriculture, industry, medicine and environment: A review. Research Journal of Medicinal Plant. 5: 230-245.

  24. Oh, A.N. and Hristov, J. (2016). Effects of Plant-derived Bio-active Compounds on Rumen Fermentation, Nutrient Utilization, Immune Response and Productivity of Ruminant Animals. In: Medicinal and Aromatic Crops: Production, Phytochemistry and Utilization. [Jeliazkov, V.D., Zheljazkov, C.L. (eds)]. American Chemical Society Publications, Washington DC, USA. pp. 167-186. 

  25. Ortega, C.M. and Mendoza, M.G. (2003). Starch digestion and glucose metabolism in the ruminant: A review. Interciencia.  28(7): 380-386. 

  26. Patra, A.K., Min, B.R. and Saxena, J. (2012). Dietary tannins on microbial ecology of the gastrointestinal tract in ruminants. Biotechnology Reports. 17: 126-136. 

  27. Rao, S.B., Jash, S., Dineshkumar, D., Krishnamoorthy, P., Elangovan, A.V., Sivaram, M., Parthipa, S. and Selvaraju, S. (2016). Influence of detoxified neem seed cake on diet digestibility, body weight change, hormonal profiles, immune response and testicular gene expression in male sheep. Animal Feed Science and Technology. 211: 41-49.

  28. Razo-Ortíz, P.B., Mendoza-Martínez, G.D., Silva, G.V., Osorio-Teran, A.I., González-Sánchez, J.F., Hernández-García, P.A., de la Torre-Hérnandez, M.E. and Espinosa-Ayala, E. (2020). Polyherbal feed additive for lambs: Effects on performance, blood biochemistry and biometry. Journal of Applied Animal Research. 48(1): 419-424.

  29. Sardar, P., Neelam, K. and Kaur, H. (1997). Influence of Livol supplementation on rumen fermentation in cattle. Indian Journal of Animal Nutrition. 13(3): 189-192.

  30. Scalbert, A. (1991). Antimicrobial properties of tannins. Phytochemistry. 30(12): 3875-3883. 

  31. Scarth, J.P., Clarke, A., Teale, P., Mill, A., Macarthur, R. and Kay, J. (2011). Detection of endogenous steroid abuse in cattle: Results from population studies in the UK. Food Additives and Contaminants: Part A. 28(1): 44-61. 

  32. van Soest, P.J., Robertson, J.B. and Lewis, B.A. (1991). Methods for dietary fiber, neutral detergent fiber and non starch polysaccharides in relation to animal nutrition. Journal of Dairy Science. 74(10): 3583-3597.

  33. Wanapat, M., Khejornsart, P., Pakdee, P. and Wanapat, S. (2008). Effect of supplementation of garlic powder on rumen ecology and digestibility of nutrients in ruminants. Journal of the Science of Food and Agriculture. 88(13): 2231-2237. 

  34. Wanapat, M., Kongmun, P., Poungchompu, O., Cherdthong, A., Khejornsart, P., Pilajun, R. and Kaenpakdee, S. (2012). Effects of plants containing secondary compounds and plant oils on rumen fermentation and ecology. Tropical Animal Health and Production. 44(3): 399-405.

  35. Wheller, G.E. (1994). Evaluation of a HerbMix as a feed ingredient for optimizing liver function and growth in veal calves. Indian Journal of Indigenous Medicines. 10(2): 27-30.

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