Indian Journal of Animal Research

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Research Article

Indian Journal of Animal Research, volume 52 issue 8 (august 2018) : 1107-1112

Association of the CSN1S1 gene polymorphism with milk composition traits in Jamunapari goat

Pallavi Singh, M.K. Singh, P.K. Rout, M.S. Dige
1Central Institute for Research on Goats, Makhdoom, Mathura-281 122, Uttar Pradesh, India
Cite article:- Singh Pallavi, Singh M.K., Rout P.K., Dige M.S. (2018). Association of the CSN1S1 gene polymorphism with milk composition traits in Jamunapari goat. Indian Journal of Animal Research. 52(8): 1107-1112. doi: 10.18805/ijar.B-3176.

ABSTRACT

The genetic polymorphism of the CSN1S1 (casein alpha-s1) locus was investigated in Jamunapari goat breed by Sodium Dodicyle Sulphate – Poly Acrylamide Gel Electrophoresis (SDS-PAGE) and Polymerase Chain Reaction – Restriction Fragment Length Polymorphism (PCR-RFLP). A total of 122 milk and blood samples were collected. The SDS-PAGE pattern revealed the presence of four variants of CSN1S1 locus namely, A, B, E and F. The A allele of a-s1 – casein was observed in the majority of goats. The genotypic frequency of the AA, BB, BE, AF and FF genotypes of CSN1S1 locus were 0.437, 0.175, 0.250, 0.125 and 0.012, respectively. The PCR-RFLP was performed, six different variant groups of F allele (224)bp, the B/E allelic pair (161+63) bp, A/0 allelic pair (150+63) bp, AD allelic pair (212+150+63) bp, AF allelic pair (224+150+63) bp and BF allelic pair (224+160+63) were obtained after restriction digestion with XmnI. The genotypic frequency of AA, AD, AF, BB, BE, BF and BC genotypes were 0.274, 0.114, 0.147, 0.098, 0.163, 0.081 and 0.163, respectively. The allelic frequency of A, B, C, D, E and F were 0.360, 0.303, 0.081, 0.057, 0.081 and 0.114, respectively. The present study showed that AA genotype had significant effect on total protein content on the milk and AD genotype had significant effect on 90 day milk yield in Jamunapari goat. Thus the CSN1S1 genotype information in Jamunapari could be utilised in selection strategies, in order to select genetic stock for the production of goat milk with higher protein content in marker assisted selection to increase the rate of genetic gain.

REFERENCES

  1. Avondo M, Pennisi P, Lanza M, Pagano R I, Valenti B, Gregorio P D, Angelis A D, Giorgio D and Trana D A. (2015). Effect of the a s1-casein genotype and its interaction with diet degradability on milk production, milk quality, metabolic and endocrinal response of Girgentana goats. Small Rum. Res. 123: 136-141.
  2. Bevilacqua C, Ferranti P, Garo G, Veltri C, Lagonigro R, Leroux C, Pietrola E, Addeo F, Pilla F, Chianese L and Martin P. (2002). Interallelic recombination is probably responsible for the occurrence of a new alpha(s1)-casein variant found in the goat species. Europ. J. Biochem. 269: 1293-1303.
  3. Boulanger A, Grosclaude F and Mahé M F. (1984). Polymorphisme des caséines As1 et As2 de la chèvre (Capra hircus). Gent. Sel. Evol. 16: 157–176.
  4. Bozkaya F, Gurler S and YerturkM. (2013). Genetic variability of CSN1S1 gene in Goat populations raised in southeastern region of Turkey. Kafkas Univ Vet Fak Derg. 19(1): 147-152.
  5. Brignon G, Mahe M F, Ribadeau Dumas B, Mercier J C and Grosclaude F. (1990). Two of the three genetic variants of goat as1-casein which are synthesized at a reduced level have an internal deletion possibly due to altered RNA splicing. Europ. J. Biochem. 193: 237-241.
  6. Caravaca F, Amills M, Jordana J, Angiolillo A, Aguera P, Aranda C, Menendez-Buxadera A, Sanchez A, Carrizosa J, Urrutia B, Sanchez A and Serradilla J M. (2008). Effect of as1-casein (CSN1S1) genotype on milk CSN1S1 content in Malaguena and Murciano-Granadina goats. J. Dairy Res. 75: 481-484.
  7. Farrell H M Jr, Jimenez-Flores R, Bleck GT, Brown E M, Butler J E, Creamer L K, Hicks C L, Hollar C M, Ng-Kwai-Hang K F and Swaisgood H E. (2004). Nomenclature of the proteins of cows’ milk – sixth revision. J. Dairy Res. 87: 1641-1674. 
  8. Feligini M, Frati S, Cubric A, Curik V, Brambilla A, Parma P, Curik I, Greppi G F and Enne G. 2005. Caprine as1-casein polymorphism: characterization of A, B, E and F variants by means of various biochemical and molecular techniques. Food Tech. Biotech. 432: 123-132.
  9. Ferretti L, Leone P and Sgaramella V. (1990). Long range restriction analysis of the bovine casein genes. Nucleic Acid Res. 18: 6829-6833.
  10. Giambra I J, Brandt H and Erhardt G. (2014). Milk protein variants are highly associated with milk performance traits in East Friesian Dairy and Lacaune sheep. Small Rum. Res. 121: 382-394.
  11. Grosclaude F and Martin P. (1997). Casein polymorphism in the goat. Pages 241-253 in: Proc. IDF Seminar, Milk Protein Polymorphism, Palmerston North (New Zealand), February 1997. IDF, Brussels,.
  12. Grosclaude F, Ricordeau G, Martin P, Remuef F, Vassal L and Bouillon J. (1994). Du gene au formagee: le polymorphismie de la caseine a-    s1 caprine, ses effets, son evolution. INRA Prod. Animales 7: 3-19.
  13. Kalamaki S M, Gelasakis A and Arsenos G. (2014). Genetic polymorphism of the CSN1S1 gene in the Greek-indigenous Skopelos goat. Proc. 4th ISOFAR Scientific Conf. ‘Building Organic Bridges’, at the Organic World Congress 2014, 13-15 Oct., Istanbul, Turkey.
  14. Kumar A. (2005). Molecular characterization of milk protein polymorphism in Indian goats. Ph.D. Thesis. Central Institute for Research on Goats, Makhdoom, Farah, Mathura, U.P. 
  15. Kumar A, Rout P K, Mandal A and Roy R. (2007). Identification of the CSN1S1 allele in Indian goats by the PCR-RFLP method. Animal. 1(8): 1099-1104.
  16. Martin P. (1993). Genetic polymorphism of the caprine lactoproteins. Milk. 735: 511-532. 
  17. Mastrangelo S, Sardina M T, Tolone M and Portolano B. (2013). Genetic Polymorphism at the CSN1S1 gene in Girgentana dairy goat breed. Animal Prod. Sci. http://dx.doi.org/10.1071/AN12242.
  18. Medrano J F and Sharrow L. (1989). Milk protein typing of bovine mammary gland tissue used to generate a complementary deoxyribonucleic acid library. J. Dairy Sci. 72: 3190-3196.
  19. Montalbano M, Tortorici L, Mastrangelo S, Sardina M T and Portolano B. (2014). Development and validation of RP-HPLC method for the quantitative estimation of ás1- genetic variants in goat milk, Food Chem. 156: 165-169.
  20. Othman O E, El-Fiky S A, Hassan N A, Mahfouz E R and Balabel E A. (2013). Genetic polymorphism detection of two a-Casein genes in three Egyptian sheep breeds. J Genetic Eng. and Biotech. 11: 129-134.
  21. Pazzola M, Dettori M L, Pira E, Paschino P and Vacca G M. (2014). Effect of polymorphisms at the casein gene cluster on milk renneting properties of the Sarda goat. Small Rum. Res. 117: 124-130.
  22. Prakash K, Rout P K, Shukla S N, Mandal A and Roy R. (2002). Genetics of milk protein variants in different Indian goats. Page 168 in Proc. Xth International Congress of Asian-Australian Association of Animal Production Societies (AAPS), 22-24 September, New Delhi, p. 168.
  23. Ramunno I, Rando A, Di Gregorio P, Massari M, Blasi M and Masina P, (1991). Genetic structre of some goat populations reared in Italy at the as1 locus. IX Congresso Nazionale ASP. 579-589
  24. Ramunno L, Cosenza G, Rando A, Pauciullo A, Illario R, Gallo D, Di Bernardino D and Masina P. (2005). Comparative analysis of gene of goat CSN1S1 F and N alleles and characterization of CSN1S1 transcript variants in mammary gland. Gene. 345: 289-299.
  25. Raymond M and Rousset F. (1995). GENEPOP (version 3.1 d): population genetics software for extract test and ecumenicism. J. Hered. 86, 248-249.
  26. Remeuf F. (1993). Influence du polymorphisme genetique de la caseine as1 caprine sur les caracteristiques physico-chimiques et technologiques. Lait. 73, 549-557.
  27. Rodrigues M T, Soares M A M, Zacaro A A, Silva M M C, Garcia O S R and Magalhaes A C M. (2015). Differences in the defective alleles E and F for the locus CSN1S1 in goats affects the profile of milk caseins. Small Rum. Res.. 123, 47-54. 
  28. Rout P K, Mandal A, Singh M K, Roy R, Sharma N and Haenlein G F W. (2004). Jamunapari: a dairy goat breed in India. Dairy Goat J. (USA) 82, 37-39. 
  29. Soares M A M, Rodrigues M T, Mognol G P, Riberio L F C, Conceicao Silva J L and Costa Brancalhao R M. (2009). Polymorphism of alpha s1 casein gene in a dairy goat herd in the southeastern region of Brazil. Revista Brasileira de Zootecnia. 38(6), 1026-1032.
  30. Thangaraj K, Joshi M B, Reddy A G, Gupta N J, Chakraborthy B and Singh L. (2002). CAG repeat expansion in androgen receptor gene is not associated with male infertility in Indian populations. J. Andrologia. 23, 815-818.
  31. Threadgill D W and Womack J E. (1990). Genomic analysis of the major bovine milk protein genes. Nucleic A. Res. 18, 6935-6942.
  32. Vassal L, Delacroix-Buchet A and Bouillon J. (1994). Influence des variants AA, EE, et FF de la caseine as1 caprine sur le rendement fromager et les caracteristiques sensorielles des fromages traditionels: Premieres observations. Lait. 74, 89-103.
  33. Weir B S. (1996). Estimating gene frequency. In genetic data analysis II (ed. BS Weir), pp. 31-49. Sinauer Associates, Inc., Sunderland.
  34. Yue X P, Zhang X M, Wang W, Ma R N, Deng C J, Lan X Y, Chen H, Li F, Xu X R, Ma Y and Lei C Z. (2011). The CSN1S1 N and F alleles identified by PCR-SSCP and their associations with milk yield and composition in Chinese dairy goats. Mole. Biol. Reprod. 38, 2821–2825. 
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