Indian Journal of Animal Research

  • Chief EditorK.M.L. Pathak

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Indian Journal of Animal Research, volume 53 issue 2 (february 2019) : 222-226

mRNA expression of ANT genes, productive performance and nitrogen excretion of laying hen by feeding low protein diets and amino acid supplementation

W. Loongyai, S. Suptavitanakit, C. Rakangthong, C. Bunchasak
1Department of Animal Science, Faculty of Agriculture at Kasetsart University, Bangkok 10900, Thailand.
Cite article:- Loongyai W., Suptavitanakit S., Rakangthong C., Bunchasak C. (2019). mRNA expression of ANT genes, productive performance and nitrogen excretion of laying hen by feeding low protein diets and amino acid supplementation. Indian Journal of Animal Research. 53(2): 222-226. doi: 10.18805/ijar.B-1000.
The study investigated the effects of low protein diet and amino acid supplementation on production performance of laying hens from 49–57 weeks. A total of 135 chickens were divided into 3 groups: 17%CP, 15%CP and 15%CP supplemented with amino acid (AA). The results showed that the 15% CP group consumed less feed (P<0.01). Adding AA to 15%CP improved feed intake, Met intake, BWG, egg mass (P<0.01) and egg production (P<0.05), while FCR and egg quality were not affected. The percentage of abdominal fat and ANT gene expression were highest in the 15%CP + AA group (P<0.05). Nitrogen retention in manure of 15% CP + AA hens was lower than in 17%CP (P<0.01). These results indicated that the reduced-CP diets supplemented with AA improved production performances and decreased nitrogen excretion. 
  1. Abd El-Maksoud, A., El-Sheikh, S.E.M., Salama A.A. and Khidr, R.E. (2011). Performance of local laying hens as affected by low protein diets and amino acids supplementation. Egypt. Poult.Sci. 31: 249-258.
  2. Akbarian, A., Golian, A., Kermanshahi, H., De Smet, S. and Michiels, J. (2014). Antioxidant enzyme activities, plasma hormone levels and serum metabolites of finishing broiler chickens reared under high ambient temperature and fed lemon and orange peel extracts and Curcuma xanthorrhiza essential oil. J.Anim.Physiol. Anim. Nutr. (Berl). 99(1): 150-162.
  3. Bottje, W. and Carstens, G.E. (2009). Association of mitochondrial function and feed effciency in poultry and livestock species. J.Anim.Sci. 87: 48-63.
  4. Conde-Aguilera, J.A., Cobo-Ortega, C., Tesseraud, S., Lessire, M., Mercier, Y. and Milgen, J. (2013). Changes in body composition in broilers by a sulfur amino acid deficiency during growth. Poult.Sci. 92: 1266-1275.
  5. Del Vesco, A.P., Eliane, G., Neto, A.R.O., Rossi, R.M., Soares, M.A.M. and Silva, S.C.C. (2013). Effect of methionine supplementation on mitochondrial genes expression in the breast muscle and liver of broilers. Livestock Sci. 151: 284-291.
  6. Gasparino, E., Del Vesco, A.P., Voltolini, D.M., Do Nascimento, C.S., Batista, E., et al., (2014). The effect of heat stress on GHR, IGF-    I, ANT, UCP and COXIII mRNA expression in the liver and muscle of high and low feed efficiency female quail. Br.Poult.Sci. 55: 466-473.
  7. Griffith, O.W. and Meister, A. (1985). Origin and turnover of mitochondrial glutathione. 82(14): 4668-4672.
  8. Iyayi, E.A., Aderemi, F.A., Ladele, O.O. and Popoola, A.S. (2014). Effects of low protein diets supplemented with high amino acids (methionine or lysine) on performance of broilers. USA. J.Agri. 4: 525-531.
  9. Kamran, Z., Sarwar, M., Nisa, M., Nadeem, M.A., Mahmood, S., Babar, M.E. and Ahmed, S. (2008). Effect of low protein dietshaving constant energy-to-protein ratio on performance and carcass characteristics of broiler chickens from one to thirty five days of age. Poult.Sci. 87: 468-474.
  10. Keshavarz, K. and Austic, R.E. (2004). The use of low-protein, low-phosphorus, amino-acid and phytase supplemented diets on laying hen performance and nitrogen and phosphorus excretion. Poult.Sci. 83: 75-83.
  11. Latshaw, J.D. and Zhao, L. (2011). Dietary protein effects on hen performance and nitrogen excretion. Poult.Sci. 90: 99-106.
  12. Mahimairaja, S., Bolan, N., Hedley, M. J. and Macgregor, A.N. (1990). Evaluation of methods of measurement of nitrogen in poultry and animal manures. Nutr. Cycl.Agroecosys. 24(3):141-148.
  13. Meluzzi, A., Sirri, F., Tallarico, N. and Franchini, A. (2001). Nitrogen retention and performance of brown laying hens on diets with different protein content and constant concentration of amino acids and energy. Br. Poult. Sci. 42: 213-217.
  14. Naga Raja Kumari, K., Ravinder Reddy, V., Chinni Preetham, V., Srinivas Kumar, D., Sen, A.R and Rama Rao S.V. (2017) Effect of feeding different levels of lysine and protein on the performance of WLH layers. Indian J. Anim. Res. 51 (5): 901-905. 
  15. National Research Council. (1994). Nutrition Requirement of Poultry. 9 th ed. National Academy of science. Washington, D.C.
  16. Neto, M.G., Pesti, G. and Bakalli, R. (2000). Influence of dietary protein level on the broiler chicken’s response to methionine and betaine supplements. Poult.Sci. 79:1478-1484.
  17. Novak, C., Yakout, H. and Scheideler, S. (2006). The effect of dietary protein level and total sulfur amino acid: lysine ratio on egg production parameters and egg yield in Hy-Line W-98 hens. Poult.Sci. 85: 2195-2206.
  18. Ojano-Dirain, C., Toyomizu, M., Wing, T., Cooper, M. and Bottje, W.G. (2007). Gene expression in breast muscle and duodenum from low and high feed efficient broilers. Poult.Sci. 86: 372-381.
  19. Prakash, B., Rama Rao, S.V., Raju, M.V.L.N. and Verma, S.K. (2018) Effect of different levels of Amino acids in low protein diets on egg production, anti-oxidant response and immune parameters in Dahlem Red Layers. Indian J. Anim. Res. 1-4.
  20. Raju, M.V.L.N., Chakrabarti, P.P., Rama Rao, S.V., Rao, B.V.S.K., Panda, A.K., Sujatha, V. (2017). Effects of dietary inclusion of lysolecithin from rice bran oil on performance, serum biochemical profile, organ weights, immune response and nutrient digestibility in broiler chicken. Indian J. Anim. Res. 51(4): 700-705.
  21. Rama Rao, S.V., Ravindran, V., Srilatha, T., Panda, A.K. and Raju, M.V.L.N. (2011). Effect of dietary concentrations of energy, crude protein, lysine and methionine on the performance of White Leghorn layers in the tropics. J.Applied Poult. Res. 20: 528-541.
  22. Saki, A.A., Naseri, H.R., Tabatabaei, M.M., Zamani, P., Haghight, M. (2012). Estimates of methionine and sulfur amino acid requirements for laying hens using different models. Rev.Bras.Cienc.Avic. 14(3): 159-232.
  23. SAS. (2004). SAS Procedures Guides, Release 9 Edition. SAS Institute Inc., Cary, North Carolina.
  24. Schutte, J.B., de Jong, J. and Bertram, H.L. (1994). Requirement of the laying hen for sulfur amino acids. Poult. Sci. 73: 274-280.
  25. Shao, D., Yiru S., Xu Z., Qiang W., Yan H., Shourong, S. and Haibing, T. (2017). Low-protein diets with balanced amino acids reduce nitrogen excretion and foot pad dermatitis without affecting the growth performance and meat quality of free-range yellow broilers. Ital.J.Anim.Sci. 17(3): 698-705.
  26. Shafey, T.M. (1996). The relationship between age and egg production, egg components and lipoprotein, lipids and fatty acids of the plasma and eggs of laying hens. J.Appl. Anim.Res. 10: 155-162.
  27. Tenesa, M., Loh, T.C., Foo, H.L., Samsudin, A.A., Mohamad, R. and Raha, A.R. (2016). Effects of feeding different levels of low crude protein diets with different levels of amino acids supplementation on layer hen performance. Pertanika J.Trop.Agric.Sci. 39(4): 543-555.
  28. Yakout, H.M. (2010). Effects of reducing dietary crude protein with amino acids supplementation on performance of commercial white leghorn layers during the second production period. Egypt. Poult. Sci. J. 30: 961-974.
  29. Zhang, F., Saha, S., Shabalina, S.A. and Kashina, A. (2010). Differential arginylation of actin isoforms is regulated by coding sequence-dependent degradation. Science. 329:1534-1537. 

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