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Research Article

Agricultural Science Digest, volume 41 issue 2 (june 2021) : 307-312

Comparative Analysis of In vitro Antimicrobial and Antioxidant Potential of Cinnamomum tamala Extract and their Essential Oils of Two Different Chemotypes

Gaurav Naik, Syed Zafar Haider, Ujjwal Bhandari, Hema Lohani, Nirpendra Chauhan
1Centre for Aromatic Plants (CAP), Industrial Estate, Selaqui-248 011, Dehradun, Uttarakhand, India.
Cite article:- Naik Gaurav, Haider Zafar Syed, Bhandari Ujjwal, Lohani Hema, Chauhan Nirpendra (2021). Comparative Analysis of In vitro Antimicrobial and Antioxidant Potential of Cinnamomum tamala Extract and their Essential Oils of Two Different Chemotypes. Agricultural Science Digest. 41(2): 307-312. doi: 10.18805/ag.D-5187.

ABSTRACT

Background: The essential oils of aromatic plants have wide range of biological applications. Natural food preservatives have been always a demanding for food industries in both developed and developing countries to prevent bacterial growth in food stuffs. Therefore, focused on Cinnamon leaves essential oils components against food pathogens have been investigated to confirm its potential use in food products. 
Methods: The antimicrobial activity of two Cinnamon leaves oils and extracts (T-2 and T-19) were examined by disc diffusion assay and the minimum inhibitory concentration by two-fold serial dilution method against foodborn pathogenic microorganisms i.e. E.coli (MTCC 723), B. Cereus (MTCC 430), S. aureus (MTCC 3381), S. typhi (MTCC 734) and C. perfringens (MTCC 1349). The antioxidant activity of both essential oils and extract was determined by DPPH assay. The chemical profiling of Cinnamon essential oils were determined by gas chromatography (GC) and gas chromatography-mass spectrometry (GC-MS). 
Result: The antimicrobial activity of both Cinnamon leaves oils and extract were evaluated by disc diffusion assay and it showed that in essential oils and extracts exhibited the highest zone of inhibition (ZOI) against S. aureus and E.coli. Minimum inhibitory concentration (MIC) of both oils and extracts ranged from 0.156 mg/ml to 5mg/ml and the antioxidant properties of oils and extract of cinnamaldehyde type Cinnamon possessed the highest antioxidant activity than linalool type. The chemical constituent of Cinnamon oil was analyzed by gas chromatography (GC) and gas chromatography-mass spectrometry (GC-MS) revealed that T-2 contains cinnamaldehyde (75%) and T-19 contains Linalool (63.77%) were found as major constituents. Therefore the results shows that essential oil of cinnamaldehyde type Cinnamon could be a potential rich source of natural antioxidants and also more effective against food borne pathogens than linalool type and could be used as natural antibacterial agents in food preservation.

REFERENCES

  1. Adams, R.P. (2007). Identification of Essential Oil Components by Gas Chromatograph/Mass Spectrometry. Allured Publishing Corp. Carol Stream. IL. USA.
  2. Ait-Ouazzou, A., Cherrat, L., Espina, L., Loran, S., Rota, C. and Pagan, R. (2011). The antimicrobial activity of hydrophobic essential oil constituents acting alone or in combined processes of food preservation. Innovative Food Science and Emerging Technologies. 12: 320-329. 
  3. Al-Jiffri, O., El-Sayed, Z. and Al-Sharif, F. (2011). Urinary tract infection with E. coli and antibacterial activity of some plant extract. International Journal of Microbiology Research. 2: 1-7.
  4. Anderson, R.A., Broadhurst, C.L., Polansky, M.M., Schmidt, W.F., Khan, A., Flanagan, V.P., Schoene, N.W. and Graves, D.J. (2004). Isolation and characterization of polyphenol type-A polymers from Cinnamon with insulin-like biological activity. Journal of Agricultural and Food Chemistry. 52(1): 65-70.
  5. Avies, N.W. (1990). Gas Chromatography Retention Indies of Monoterpenes and Sesquiterpenes on Methyl Silicone and Carbowax 20M Phases. Journal of Chromatography A. 503: 1-24.
  6. Brand-Williams W., Cuvelier, M.E. and Berset, C. (1995). Use of free radical method to evaluate antioxidant activity. Lebensmittel Wissenschaft and Technologie. 28: 25-30.
  7. Brierley, S.M. and Klber, O. (2011). Use of natural products in gastrointestinal therapies. Current Opinion in Pharmacology. 11: 604-11.
  8. Dobre, A.A., Gagiu, V. and Petru, N. (2011). Antimicrobial activity of essential oils against food-borne bacteria evaluated by two preliminary methods. Romanian Biotechnological Letters. 16(6): 119-125.
  9. Dormans, H.J.D. and Deans, S.G. (2000). Antimicrobial agents from plants: Antibacterial activity of plant volatile oils. Journal of Applied Microbiology. 88: 308-316. 
  10. Elaissi, A., Salah, K.H., Mabrouk, S., Larbi, K.M., Chemli, R. and Harzallah-Skhir, F. (2011). Antibacterial activity and chemical composition of 20 Eucalyptus species’ essential oils. Food Chemistry. 129: 1427-1434.
  11. Griffin, G.S., Wyllie, G.S., Markham, L.J. and Leach, D.N. (1999). The role of structure and molecular properties of terpenoids in determining their antimicrobial activity. Flavour Fragrance Journal. 14: 322–332.
  12. Huang, T.C., Fu, H.Y., Ho, C.T., Tan, D., Huang, Y.T. and Pan, M.H. (2007). Induction of apoptosis by cinnamaldehyde from indigenous Cinnamon Cinnamomumosmophloeum Kaneh through reactive oxygen species production, glutathione depletion and caspase activation in human leukemia K562 cells. Food Chemistry. 103: 434-443.
  13. Jo, Y.J., Chun, J.Y., Kwon, Y.J., Min, S.G., Hong, G.P.P. and Choi, M.J. (2015). Physical and antimicrobial properties of trans- cinnamaldehyde nano emulsions in water melon juice, LWT - Food Science and Technology. 60(1): 444-451.
  14. Kallel, I., Hadrich, B., Gargouri, B., Chaabane, A., Lassoued, S., Gdoura, R., Bayoudh, A. and Massaoud, B. E. (2019). Optimization of Cinnamon (Cinnamomum zeylanicum Blume) Essential oil Extraction: Evaluation of Antioxidant and Antiproliferative effects. doi.org/10.1155/2019/6498347.
  15. Kapoor, I.P.S., Singh, S. Singh, G., Isidorov, V. and Szczepaniak, L. (2009). Chemistry, antimicrobial and antioxidant potentials of Cinnamomum tamala Nees et Eberm. (Tejpat) essential oil and oleoresins, Natural Product Rediance. 8:106-116. 
  16. Lopes, F.A., Soares, N.F.F., Pires, L.C.C., Silva, W.A., Junior, J.C.B. and Medeiros, E.A.A. (2014). Conservation of bakery products through cinnamaldehyde antimicrobial films. Packaging Technology and Science. 27(4): 293-302. 
  17. Marongiu, B., Piras, A., Porcedda, S., Tuveri, E., Sanjust, E., Meli, M., Sollai, F., Zucca, P. and Rescigno, A. (2007). Supercritical CO2 extract of Cinnamomum zeylanicum: chemical characterization and antityrosinase activity. Journal of Agricultural and Food Chemistry. 55(24): 10022-10027. 
  18. Miladi, H., Zmantar, T., Chaabouni, Y., Fedhila, K., Bakhrouf, A., Mandouani, K. and Chaieb, K. (2016). Antibacterial and efflux pump inhibitors of thymol and carvacrol against food-borne pathogens. Microbial Pathogenesis. 99: 95-100. 
  19. Mishra, A.K., Singh, B.K. and Pandey, A.K. (2010). In vitro-antibacterial activity and phytochemical profiles of Cinnamomum tamala (Tejpat) leaf extracts and oil. Review in Infection. 1(3): 134-139.
  20. Mith, H., Dure, R., Delcenserie, V., Zhiri, A., Daube, G.and Clinquar, A. (2014). Antimicrobial activities of commercial essential oils and their components against foodborne pathogens and food spoilage bacteria. Food Science and Nutrition. 2(4): 403-416.
  21. Moarefian, M., Barzegar, M., Sattari, M. (2013). Cinnamomum zeylanicum essential oil as a natural antioxidant and antibacterial in cooked sausage. Journal of Food Biochemistry. (37): 62-69. 
  22. Mohamed, M.I.H., Mona, Y.O., Madeha, O.G. and Wafaa, A.H. (2015). Some Microbiological Studies on Aqueous and Methanolic Extracts of Cinnamon (C. zeylanicum). Indian Journal of Research. 4(8): 388-391.
  23. Nimje, P.D., Garg, H., Gupta, A., Srivastava, N., Katiyar, M. and Ramalingam, C. (2013). Comparison of antimicrobial activity of Cinnamomum zeylanicum and Cinnamomum cassia on food spoilage bacteria and water borne bacteria. Der Pharmacia Lettre. 5(1): 53-59.
  24. Oliveira, M.M., Brugnera, D.F., Nascimento, J.A., Batista, N.N and Piccoli, R.H. (2012). Cinnamon essential oil and cinnamaldehyde in the control of bacterial biofilms formed on stainless steel surfaces. European Food Research and Technology. 234: 821-832.
  25. Padalia, R.C., Verma, R.S., Sah, A., Karki, Chauhan, N.A., Sakia, D. and Krishna, B. (2012). Study on Chemotypic Variations in Essential Oil of Cinnamomumtamala (Buch.-Ham.) Nees et Eberm. and their Antibacterial and Antioxidant Potential. Journal of Essential Oil Bearing Plants. 15 (5): 800-808. 
  26. Pandey, A.K. and Mishra, A.K. (2012). Antifungal and antioxidative potential of oil and extracts derived from leaves of Indian spice plant Cinnamomum tamala. Cell Molecular Biology. 58(1): 142-147.
  27. Peng, X., Cheng, K.W., Ma, J., Chen, B., Ho, C.T., Lo, C., Chen, F. and Wang, M. 2008. Cinnamon bark proanthocyanidins as reactive carbonyl scavengers to prevent the formation of advanced glycation end products. Journal of Agricultural and Food Chemistry. 56(6): 1907-1911.
  28. Sachetti, G., Maietti, S., Muzzoli, M., Scaglianti, M., Manfredini, S., Radice, M. and Bruni, R. (2005). Comparative evaluation of 11 essential oils of different origin as functional antioxidants, antiradicals and antimicrobials in foods. Food Chemistry. 91: 621-632. 
  29. Sathish, K.M., Sneha, K., Won, S.W., Cho, C.W., Kim, S. and Yun, Y.S. (2009). Cinnamon zeylanicumbark extract and powder mediated green synthesis of nano-crystalline silver particles and its bactericidal activity. Colloid Surfaces. B73: 332-338.
  30. Sharma, A., Verma, R. and Ramteke, P. (2009). Antibacterial activity of some medicinal plants used by tribals against UTI causing pathogens. World Applied Sciences Journal. 7 (3): 332-339.
  31. Silva, F. and Domingues, F.C. (2017). Antimicrobial activity of Coriander oil and its effectiveness as food preservative. Critical Reviews in Food Science and Nutrition. 57: 35-47.
  32. Silva, N., Santos, T., Marinho, C., Sousa, M., Teixeira, J., Gonzalez, D., Correia, E., Igrejas, G. and Poeta, P. (2016). Effect of Mediterranean Oils and Spices against food Related Pathogenic Bacteria. Journal of food and Dairy Technology. 4(3): 52-59.
  33. Sudan, R., Bhagat, M., Gupta, S., Chitrarakha. and Devi, T. (2013). Comparative analysis of cytotoxic and antioxidant potential of edible Cinnamomum verum (bark) and Cinnamomum tamala (Indian bay leaf) Free Radicals and Antioxidants. 3: 70-73.
  34. Tajkarimi, M.M., Ibrahim, S.A. and Cliver, D.O. (2010). Antimicrobial herb and spice compounds in food. Food Control. 21: 1199-1218.
  35. Tiziana, B.M., Bamien, D.H.J., Deans, G., Cristina, F.A., Barroso, G. and Ruberto, G. (1998). Antimicrobial and antioxidant properties of some commercial essential oils. Flavour Fragrance Journal. 13: 235-244.
  36. Tyagi, A.K. and Malik, A. (2011). Antimicrobial potential and chemical composition of Eucalyptus globulus oil in liquid and vapour phase against food spoilage microorganisms. Food Chemistry. 126: 228-235.
  37. Yeh, H.F., Luo, C.Y., Lin, C.Y., Cheng, S.S., Hsu, Y.R. and Chang, S.T. (2013). Methods for thermal stability enhancement of leaf essential oils and their main Constituents from Indigenous Cinnamon (Cinnamomum osmophloeum). Journal of Agricultural and Food Chemistry. 61(26): 6293-6298.
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