Authenticity of Aloe vera and Acacia Honey on Wound and their Comparative Wound Healing Efficiency on Lacerated Wound in Rabbit

.


MATERIALS AND METHODS
Experimental animals 30 locally bred, clinically healthy male adult rabbits, weighing between 2 -2.5 kg were purchased from local market of D.I. Khan.Animals were kept in Rabbit Research House, PARC, Arid Zone Research Centre, D.I. Khan.Animals were randomly divided into 3 groups (A, B and C).

Clinical examination
All animals were kept on uniform feeding regimen and manage mental conditions in the PARC, AZRC, Rabbit Research House, DI Khan.Clinical and laboratory examination was carried out of each animal and animals showing any signs of disease were replaced with healthy ones.An acclimatization period of at least two weeks was provided to the rabbits during which 2 doses of ivermectin (@400microgram/kg; S/C) were provided to each rabbit.Moreover, to avoid the chances of pasturellosis, amoxicillin (@15mg/kg S/C) was injected to all the rabbits for three consecutive days.

Premedication and anesthesia
Each rabbit from all groups was pre-medicated by administering atropine sulphate @0.035mg/kg body weight through s/c route half an hour prior to surgical intervention.Animals were anesthetized by total parenteral (intramuscular) anesthesia using a mixture of ketamine (35mg/kg) and xylazine (5mg/kg) (Razaini et al., 2004).
Contraction % = 100 -(Area of wound on that day) x 100 Area of wound at day 0

Figure A :
Figure A: Lacerated wounds induced by sharp blunt scissor in rabbit

Fig: 2 Fig: 3
Fig: 2 Graphical comparison of Acacia honey and Aloe vera gel and pyodine on healing time

Fig: 4
Fig: 4 Graphical comparison of Acacia honey, Aloe vera gel and pyodine on wound index

Figure B :
Figure B: Lacerated wound after Acacia honey and Aloe vera application at day 10 It is reсоmmended thаt yоu test а smаll аreа first fоr аllergiс resроnses.It саn be used if there аre nо аllergiс resроnses.